anti-Rat (Rattus) ARFGAP1 antibody for Western Blotting

Recommended ARFGAP1 Antibody (supplied by: Log in to see )

Antigen
ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) Antibodies
  • ARF1GAP
  • HRIHFB2281
  • AI115377
  • Arf1gap
  • Arf GAP1
  • arf1gap
  • atfb1
  • atfb3
  • jlns1
  • k(v)7.1
  • kcna8
  • kcna9
  • kv1.9
  • kv7.1
  • kvlqt1
  • lqt
  • lqt1
  • rws
  • sqt2
  • wrs
  • ARFGAP3
  • ADP ribosylation factor GTPase activating protein 1
  • ADP-ribosylation factor GTPase activating protein 1
  • ADP ribosylation factor GTPase activating protein 1 S homeolog
  • putative ADP-ribosylation factor GTPase activating protein 1
  • ATP-dependent RNA helicase DBP9
  • ARFGAP1
  • Arfgap1
  • arfgap1.S
  • Tb11.01.6060
  • arfgap1
  • LMJF_32_1230
  • MGYG_02419
Epitope
AA 377-406, C-Term
17
13
4
4
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human, Rat (Rattus)
108
33
29
19
8
2
1
1
1
Host
Rabbit
73
35
1
Clonality
Polyclonal
Conjugate
This ARFGAP1 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
108
55
46
45
33
13
9
5
3
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Pubmed
3 references available

Available images

Western Blotting (WB) image for anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) (AA 377-406), (C-Term) antibody (ABIN388996)
Immunohistochemistry (IHC) image for anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) (AA 377-406), (C-Term) antibody (ABIN388996)
Western Blotting (WB) image for anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) (AA 377-406), (C-Term) antibody (ABIN388996)
Catalog No. ABIN388996
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.815655 ABIN2561195 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
12.579504 ABIN3183347 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
10.815655 ABIN3197892 IC IF IHC WB Rabbit Log in to see Polyclonal 0
10.815655 ABIN2970468 IF/ICC IHC WB Rabbit IgG Log in to see Polyclonal 0
10.815655 ABIN6043190 IF/ICC IHC WB Rabbit IgG full length Log in to see Polyclonal 0
7.815655 ABIN2787084 WB Rabbit Middle Region Log in to see Polyclonal 0
7.815655 ABIN1534608 IF ELISA WB Rabbit IgG AA 171-220 Log in to see Polyclonal 1
7.815655 ABIN6257929 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
7.815655 ABIN5965201 IHC WB Rabbit IgG Log in to see Polyclonal 0
7.815655 ABIN3001502 IHC WB Rabbit IgG Log in to see Polyclonal 0
7.815655 ABIN2422639 ELISA WB Rabbit IgG Log in to see Polyclonal 0
7.815655 ABIN2429235 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN357645 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 1
4.815655 ABIN4886474 WB Rabbit IgG AA 1-183 Log in to see Polyclonal 0
4 ABIN960082 WB Rabbit AA 123-139 Log in to see Polyclonal 0
4 ABIN1574785 IF ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN2883485 ELISA IF WB Rabbit IgG AA 171-220 Log in to see Polyclonal 0
1 ABIN2882987 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1841436 IHC IHC (p) WB Rabbit AA 377-406 Log in to see Polyclonal 0
1 ABIN5700220 ELISA IHC IP WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) Antibodies
Epitope AA 377-406, C-Term
(17), (13), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(108), (33), (29), (19), (8), (2), (1), (1), (1)
Host Rabbit
(73), (35), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This ARFGAP1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(108), (55), (46), (45), (33), (13), (9), (5), (3), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-ARFGAP1 Antibody

Target Details ARFGAP1 Application Details Handling References for anti-ARFGAP1 antibody (ABIN388996) Images
Specificity This ARFGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377~406 amino acids from the C-terminal region of human ARFGAP1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This ARFGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 AA from the C-terminal region of human ARFGAP1.
Clone RB4855
Isotype Ig Fraction
Plasmids, Primers & others

Target Details ARFGAP1

Product Details anti-ARFGAP1 Antibody Application Details Handling References for anti-ARFGAP1 antibody (ABIN388996) Images back to top
Antigen
Alternative Name ARFGAP1 (ARFGAP1 Antibody Abstract)
Background ARFGAP1 is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine.
Synonyms: ADP-ribosylation factor GTPase-activating protein 1, ADP-ribosylation factor 1 GTPase-activating protein, ARF1 GAP, ARF1-directed GTPase-activating protein, GAP protein, ARF1GAP
Molecular Weight 44668 DA
Gene ID 55738
UniProt Q8N6T3
Pathways p53 Signaling, ER-Nucleus Signaling, Unfolded protein response

Application Details

Product Details anti-ARFGAP1 Antibody Target Details ARFGAP1 Handling References for anti-ARFGAP1 antibody (ABIN388996) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-ARFGAP1 Antibody Target Details ARFGAP1 Application Details References for anti-ARFGAP1 antibody (ABIN388996) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-ARFGAP1 antibody (ABIN388996)

Product Details anti-ARFGAP1 Antibody Target Details ARFGAP1 Application Details Handling Images back to top
Product cited in:

Nishimura, Uchida, Yachi, Kudlyk, Lupashin, Inoue, Taguchi, Arai: "Oxysterol-binding protein (OSBP) is required for the perinuclear localization of intra-Golgi v-SNAREs." in: Molecular biology of the cell, Vol. 24, Issue 22, pp. 3534-44, 2013 Method employed by authors: Immunofluorescence (IF)

Zhang, Major, Takanashi, Camp, Nishiya, Peters, Ginsberg, Jian, Randazzo, Schultz, Moon, Ding: "Small-molecule synergist of the Wnt/beta-catenin signaling pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 18, pp. 7444-8, 2007 Method employed by authors: Western Blotting (WB)

Background publications

Huber, Rotman, Pick, Makler, Rothem, Cukierman, Cassel: "Expression, purification, and properties of ADP-ribosylation factor (ARF) GTPase activating protein-1." in: Methods in enzymology, Vol. 329, pp. 307-16, 2001

Images

Product Details anti-ARFGAP1 Antibody Target Details ARFGAP1 Application Details Handling References for anti-ARFGAP1 antibody (ABIN388996) back to top
Supplier Images
Western Blotting (WB) image for anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) (AA 377-406), (C-Term) antibody (ABIN388996) Western blot analysis of ARFGAP1 Antibody (C-term) (ABIN388996) against rat recombina...
Immunohistochemistry (IHC) image for anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) (AA 377-406), (C-Term) antibody (ABIN388996) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) (AA 377-406), (C-Term) antibody (ABIN388996) All lanes : Anti-ARFGAP1 Antibody (C-term) at 1:8000 dilution Lane 1: HepG2 whole cel...