anti-Cow (Bovine) BAI1 antibody for Immunohistochemistry

Recommended BAI1 Antibody (supplied by: Log in to see )

Antigen
Brain-Specific Angiogenesis Inhibitor 1 (BAI1) Antibodies
  • BAI1
  • GDAIF
  • B830018M07Rik
  • R75078
  • mKIAA4089
  • brain-specific angiogenesis inhibitor 1
  • adhesion G protein-coupled receptor B1
  • BAI1
  • ADGRB1
  • LOC100228084
  • Adgrb1
Epitope
C-Term
45
12
11
4
3
2
2
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
103
60
25
10
4
3
1
1
1
1
Host
Rabbit
103
1
Clonality
Polyclonal
Conjugate
This BAI1 antibody is un-conjugated
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
55
50
40
32
21
13
12
5
3
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN1048337
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.404629 ABIN213519 ELISA IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0
8.404629 ABIN2469439 IHC ELISA Rabbit N-Term Log in to see Polyclonal 0

General

Antigen Brain-Specific Angiogenesis Inhibitor 1 (BAI1) Antibodies
Epitope C-Term
(45), (12), (11), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
(103), (60), (25), (10), (4), (3), (1), (1), (1), (1)
Host Rabbit
(103), (1)
Clonality
Polyclonal
Conjugate This BAI1 antibody is un-conjugated
(4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(55), (50), (40), (32), (21), (13), (12), (5), (3), (2), (1), (1)
Supplier Log in to see

Product Details anti-BAI1 Antibody

Target Details BAI1 Application Details Handling Images
Brand IHC-plus™
Specificity Human BAI1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Bovine, Opossum, Chicken, Platypus (100%) Xenopus, Zebrafish (94%) Stickleback, Pufferfish (88%).
Purification Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from C-terminus of human BAI1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Bovine, Opossum, Chicken, Platypus (100%), Xenopus, Zebrafish (94%), Stickleback, Pufferfish (88%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details BAI1

Product Details anti-BAI1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ADGRB1 / BAI1 (BAI1 Antibody Abstract)
Background Name/Gene ID: ADGRB1
Subfamily: Orphan-B
Family: GPCR

Synonyms: ADGRB1, BAI1, GDAIF
Gene ID 575
Pathways p53 Signaling

Application Details

Product Details anti-BAI1 Antibody Target Details BAI1 Handling Images back to top
Application Notes Approved: IHC, IHC-P (14 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-BAI1 Antibody Target Details BAI1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-BAI1 Antibody Target Details BAI1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Brain-Specific Angiogenesis Inhibitor 1 (BAI1) (C-Term) antibody (ABIN1048337) Anti-BAI1 antibody ABIN1048337 IHC staining of human brain, neurons. Immunohistochem...
Immunohistochemistry (IHC) image for anti-Brain-Specific Angiogenesis Inhibitor 1 (BAI1) (C-Term) antibody (ABIN1048337) Anti-BAI1 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formali...