anti-Human BAX antibody for Enzyme Immunoassay

Recommended BAX Antibody

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • BAX-ALPHA
  • bax-A
  • xBax
  • xlbax
  • BAX
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • BCL2L4
  • BCL2 associated X, apoptosis regulator
  • BCL2-associated X protein L homeolog
  • BCL2-associated X protein
  • bcl2-associated X protein, a
  • BAX
  • bax.L
  • bax
  • baxa
  • Bax
Epitope
N-Term
84
39
31
21
21
18
15
14
11
10
10
9
8
8
7
7
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
496
229
208
84
42
29
27
20
10
6
5
4
4
3
3
2
2
1
1
1
1
1
1
1
Host
Rabbit
342
182
5
2
Clonality
Polyclonal
Conjugate
This BAX antibody is un-conjugated
17
13
12
11
10
10
6
6
6
6
6
6
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
403
201
181
143
94
89
83
42
39
22
20
13
9
5
4
2
1
1
1
1
1
1
Options

Available images

Catalog No. ABIN356811
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
7 ABIN5611595 EIA IF IHC (p) Rabbit IgG AA 145-157, Internal Region Polyclonal 0
7 ABIN265325 EIA IHC (p) WB Rabbit Polyclonal 0
5.5 ABIN2716521 EIA IHC (p) WB Rabbit IgG Polyclonal 2
1 ABIN5554592 EIA IF WB Rabbit IgG N-Term Polyclonal 0

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Epitope N-Term
(84), (39), (31), (21), (21), (18), (15), (14), (11), (10), (10), (9), (8), (8), (7), (7), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(496), (229), (208), (84), (42), (29), (27), (20), (10), (6), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(342), (182), (5), (2)
Clonality
Polyclonal
Conjugate This BAX antibody is un-conjugated
(17), (13), (12), (11), (10), (10), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(403), (201), (181), (143), (94), (89), (83), (42), (39), (22), (20), (13), (9), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
Supplier

Product Details anti-BAX Antibody

Target Details BAX Application Details Handling References for anti-BAX antibody (ABIN356811) Images
Specificity This antibody is specific to Bax (BH3 Domain).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody was developed against a synthetic peptide selected from the N-terminal region (BH3 domain) of human Bax.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling References for anti-BAX antibody (ABIN356811) Images back to top
Antigen
Alternative Name Bax (BAX Antibody Abstract)
Background Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of the Bax gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.Synonyms: Apoptosis regulator BAX, BCL2-associated X protein, BCL2L4, Bcl-2-like protein 4, Bcl2-L-4
Gene ID 581, 9606
UniProt Q07812
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling References for anti-BAX antibody (ABIN356811) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details References for anti-BAX antibody (ABIN356811) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-BAX antibody (ABIN356811)

Product Details anti-BAX Antibody Target Details BAX Application Details Handling Images back to top
Background publications

Liu, Newland, Jia: "Bax conformational change is a crucial step for PUMA-mediated apoptosis in human leukemia." in: Biochemical and biophysical research communications, Vol. 310, Issue 3, pp. 956-62, 2003

Roucou, Guo, Zhang, Goodyer, LeBlanc: "Cytosolic prion protein is not toxic and protects against Bax-mediated cell death in human primary neurons." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 40877-81, 2003

Cao, Deng, May: "Cleavage of Bax to p18 Bax accelerates stress-induced apoptosis, and a cathepsin-like protease may rapidly degrade p18 Bax." in: Blood, Vol. 102, Issue 7, pp. 2605-14, 2003

McJilton, Van Sikes, Wescott, Wu, Foreman, Gregory, Weidner, Harris Ford, Morgan Lasater, Mohler, Terrian: "Protein kinase Cepsilon interacts with Bax and promotes survival of human prostate cancer cells." in: Oncogene, Vol. 22, Issue 39, pp. 7958-68, 2003

Bidère, Lorenzo, Carmona, Laforge, Harper, Dumont, Senik: "Cathepsin D triggers Bax activation, resulting in selective apoptosis-inducing factor (AIF) relocation in T lymphocytes entering the early commitment phase to apoptosis." in: The Journal of biological chemistry, Vol. 278, Issue 33, pp. 31401-11, 2003

Images

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Supplier Images
 image for anti-BCL2-Associated X Protein (BAX) (N-Term) antibody (ABIN356811) anti-BCL2-Associated X Protein (BAX) (N-Term) antibody
 image for anti-BCL2-Associated X Protein (BAX) (N-Term) antibody (ABIN356811) anti-BCL2-Associated X Protein (BAX) (N-Term) antibody (Image 2)