anti-Human BAX antibody for Immunocytochemistry

Recommended BAX Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • BAX-ALPHA
  • bax-A
  • xBax
  • xlbax
  • BAX
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • BCL2L4
  • BCL2 associated X, apoptosis regulator
  • BCL2-associated X protein L homeolog
  • BCL2-associated X protein
  • bcl2-associated X protein, a
  • BAX
  • bax.L
  • bax
  • baxa
  • Bax
Epitope
AA 3-16
93
76
31
22
21
17
15
13
12
12
10
10
10
10
10
7
7
5
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey
556
240
218
122
42
31
26
23
21
9
6
5
5
3
3
2
1
1
1
1
Host
Mouse
352
240
4
Clonality
Monoclonal
Conjugate
This BAX antibody is un-conjugated
19
15
15
13
12
9
8
6
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
454
233
194
157
143
142
122
44
42
40
33
26
13
5
5
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN4283106
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN451743 ELISA FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 kappa N-Term Log in to see 6A7 3
1 ABIN213905 ICC IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN1001866 ICC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 4
1 ABIN1978903 ELISA ICC IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 0
1 ABIN1866865 ICC IHC WB Rabbit IgG AA 1-192 Log in to see Polyclonal 0
1 ABIN1732229 ICC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN4283109 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN4283139 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN4283144 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN4283137 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN4283131 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN4283132 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN4283136 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN4283146 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN4283130 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN1031261 ICC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN5949411 FACS ICC IF IHC IHC (fro) IHC (p) Janelia Fluor® 549 Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN5949412 FACS ICC IF IHC IHC (fro) IHC (p) Janelia Fluor® 646 Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN2589882 ELISA ICC IF IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN2617494 ICC IF IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Epitope AA 3-16
(93), (76), (31), (22), (21), (17), (15), (13), (12), (12), (10), (10), (10), (10), (10), (7), (7), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey
(556), (240), (218), (122), (42), (31), (26), (23), (21), (9), (6), (5), (5), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(352), (240), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BAX antibody is un-conjugated
(19), (15), (15), (13), (12), (9), (8), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(454), (233), (194), (157), (143), (142), (122), (44), (42), (40), (33), (26), (13), (5), (5), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BAX Antibody

Target Details BAX Application Details Handling References for anti-BAX antibody (ABIN4283106) Images
Specificity Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Protein G purified
Immunogen A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
Clone 2D2
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling References for anti-BAX antibody (ABIN4283106) Images back to top
Antigen
Alternative Name Bax (BAX Antibody Abstract)
Background Gene Symbol: BAX
Molecular Weight Theoretical MW: 21 kDa
Gene ID 581
UniProt Q07812
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling References for anti-BAX antibody (ABIN4283106) Images back to top
Application Notes Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, Immunohistochemistry 0.5-1 μg/mL, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mLHuman Chromosome Location: 19q13.33 Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details References for anti-BAX antibody (ABIN4283106) Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.

References for anti-BAX antibody (ABIN4283106)

Product Details anti-BAX Antibody Target Details BAX Application Details Handling Images back to top
Product cited in:

Ozer, Yenicesu, Arici, Cetin, Tuncer, Cetin et al.: "Immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in benign, borderline, and malignant serous and mucinous ..." in: Diagnostic pathology, Vol. 7, pp. 124, 2012

Images

Product Details anti-BAX Antibody Target Details BAX Application Details Handling References for anti-BAX antibody (ABIN4283106) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2-Associated X Protein (BAX) (AA 3-16) antibody (ABIN4283106) Immunohistochemistry-Paraffin: Bax Antibody (2D2) [NBP2-29468] - Formalin-fixed, para...