anti-Monkey BAX antibody for Flow Cytometry

Recommended BAX Antibody

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • BAX-ALPHA
  • bax-A
  • xBax
  • xlbax
  • BAX
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • BCL2L4
  • BCL2 associated X, apoptosis regulator
  • BCL2-associated X protein L homeolog
  • BCL2-associated X protein
  • bcl2-associated X protein, a
  • BAX
  • bax.L
  • bax
  • baxa
  • Bax
Epitope
AA 3-16
84
42
31
21
21
18
15
12
11
10
9
9
8
8
7
7
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey
499
230
208
89
42
29
27
20
10
6
5
4
4
3
3
2
2
1
1
1
1
1
1
1
Host
Mouse
341
186
5
2
Clonality
Monoclonal
Conjugate
This BAX antibody is un-conjugated
17
13
12
11
10
10
6
6
6
6
6
6
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
404
201
185
146
98
89
84
42
39
22
20
13
9
5
5
5
2
1
1
1
1
1
1
Options
Catalog No. ABIN6935091
$ 242.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
13.447434 ABIN6935092 FACS IF IHC StM WB Mouse IgG1, kappa AA 3-16 SPM336 0
13.447434 ABIN6935093 FACS IHC StM Mouse IgG1, kappa BAX-962 0
13.447434 ABIN6936465 FACS IF IHC StM WB Mouse IgG1, kappa AA 3-16 2D2 0
13.447434 ABIN6936466 FACS IF IHC StM WB Mouse IgG1, kappa AA 3-16 SPM336 0
13.447434 ABIN6936467 FACS IHC StM Mouse IgG1, kappa BAX-962 0
7 ABIN6175357 FACS IF IHC (f) WB PerCP Mouse IgG1 BAX-962 0
7 ABIN6175358 FACS IF IHC (f) WB PE Mouse IgG1 BAX-962 0
7 ABIN6175350 FACS IF IHC (f) WB APC Mouse IgG1 BAX-962 0
7 ABIN6175361 FACS IF IHC (f) WB Mouse IgG1 BAX-962 0
7 ABIN3025797 FACS IF IHC (p) WB Mouse IgG1 BAX-962 0
4.9261656 ABIN451743 ELISA FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 kappa N-Term 6A7 8
4 ABIN6175319 FACS IF IHC (f) WB PerCP Mouse IgG1 AA 3-16 2D2 0
4 ABIN6175320 FACS IF IHC (f) WB PE Mouse IgG1 AA 3-16 2D2 0
4 ABIN6175312 FACS IF IHC (f) WB APC Mouse IgG1 AA 3-16 2D2 0
4 ABIN6175323 FACS IF IHC (f) WB Mouse IgG1 AA 3-16 2D2 0
4 ABIN2666260 FACS Mouse IgG1 kappa 6A7 6
4 ABIN3026586 ELISA FACS IF IHC (p) IP WB Mouse IgG1 2D2 0
4 ABIN3025800 FACS IF IHC (p) WB Mouse IgG1 AA 3-16 SPM336 0
4 ABIN3026588 ELISA FACS IF IHC (p) IP WB Mouse IgG1 2D2 0
4 ABIN3025801 FACS IF IHC (p) WB Mouse IgG1 AA 3-16 SPM336 0

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Epitope AA 3-16
(84), (42), (31), (21), (21), (18), (15), (12), (11), (10), (9), (9), (8), (8), (7), (7), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey
(499), (230), (208), (89), (42), (29), (27), (20), (10), (6), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(341), (186), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BAX antibody is un-conjugated
(17), (13), (12), (11), (10), (10), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
(404), (201), (185), (146), (98), (89), (84), (42), (39), (22), (20), (13), (9), (5), (5), (5), (2), (1), (1), (1), (1), (1), (1)
Supplier

Product Details anti-BAX Antibody

Target Details BAX Application Details Handling Images
Specificity Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified by Protein A/G
Immunogen A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
Clone 2D2
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling Images back to top
Antigen
Alternative Name BAX (BAX Antibody Abstract)
Molecular Weight 21kDa
Gene ID 581
UniProt Q07812
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling Images back to top
Application Notes

Positive Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details Images back to top
Concentration 200 μg/mL
Buffer 10 mM PBS with 0.05 % BSA & 0.05 % azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-80 °C
Storage Comment Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Expiry Date 24 months