anti-Monkey BAX antibody for Immunohistochemistry

Recommended BAX Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • BAX-ALPHA
  • bax-A
  • xBax
  • xlbax
  • BAX
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • BCL2L4
  • BCL2 associated X, apoptosis regulator
  • BCL2-associated X protein L homeolog
  • BCL2-associated X protein
  • bcl2-associated X protein, a
  • BAX
  • bax.L
  • bax
  • baxa
  • Bax
Reactivity
Human, Monkey
500
219
200
88
47
35
30
28
22
9
8
8
5
3
3
2
1
1
1
1
Host
Mouse
324
206
4
Clonality
Monoclonal
Conjugate
This BAX antibody is un-conjugated
19
14
13
11
10
8
7
6
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
393
215
142
141
120
117
100
46
40
37
13
9
5
5
4
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN3026586
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.044337 ABIN2470569 IP IHC WB Mouse IgG1 Log in to see 6A7 0
9.044337 ABIN3025800 FACS IF IHC WB Mouse IgG1 AA 3-16 Log in to see SPM336 0
1 ABIN4283106 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa AA 3-16 Log in to see 2D2 1
1 ABIN451743 ELISA FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 kappa N-Term Log in to see 6A7 3
1 ABIN4283105 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa AA 3-16 Log in to see 2D2 0
1 ABIN4283104 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa AA 3-16 Log in to see 2D2 0
1 ABIN4283123 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa AA 3-16 Log in to see 2D2 0
1 ABIN4283116 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB APC Mouse IgG1 kappa AA 3-16 Log in to see 2D2 0
1 ABIN3026587 IHC Mouse IgG1 Log in to see 2D2 0
1 ABIN4283124 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB PE Mouse IgG1 kappa AA 3-16 Log in to see 2D2 0
1 ABIN3026588 FACS IF IP IHC ELISA WB Mouse IgG1 Log in to see 2D2 0
1 ABIN3025798 IHC Mouse IgG1 Log in to see BAX-962 0
1 ABIN3025801 FACS IF IHC WB Mouse IgG1 AA 3-16 Log in to see SPM336 0

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Reactivity Human, Monkey
(500), (219), (200), (88), (47), (35), (30), (28), (22), (9), (8), (8), (5), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(324), (206), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BAX antibody is un-conjugated
(19), (14), (13), (11), (10), (8), (7), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(393), (215), (142), (141), (120), (117), (100), (46), (40), (37), (13), (9), (5), (5), (4), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-BAX Antibody

Target Details BAX Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics This antibody recognizes a protein of 21 kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2.
Purification Protein G affinity chromatography
Immunogen Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody.
Clone 2D2
Isotype IgG1

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bax (BAX Antibody Abstract)
Background This antibody recognizes a protein of 21 kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2.
Gene ID 581
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bax antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: order BSA-free format for coating,FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Bax antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-BAX Antibody Target Details BAX Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BCL2-Associated X Protein (BAX) antibody (ABIN3026586) IHC testing of Hodgkin's lymphoma stained with Bax antibody (Clone 2D2).