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|Antigen||BCL2 Binding Component 3 (BBC3) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BBC3 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-BBC3 AntibodyTarget Details BBC3 Application Details Handling Images
|Specificity||This antibody detects a 23 kDa protein corresponding to the molecular mass of PUMA on SDS PAGE immunoblots. 16 kDa band may be seen in some instances, possibly corresponding to PUMAβ.|
|Immunogen||C-terminal amino acids of Human PUMA|
Target Details BBC3Product Details anti-BBC3 Antibody Application Details Handling Images back to top
|Alternative Name||PUMA (BBC3 Antibody Abstract)|
|Background||Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis|
|Research Area||Apoptosis/Necrosis, Cancer, Metabolism|
|Pathways||p53 Signaling, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-BBC3 Antibody Target Details BBC3 Handling Images back to top
Western blot: 1: 1000. Immunoflourescence: 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BBC3 Antibody Target Details BBC3 Application Details Images back to top
|Buffer||PBS, 0.02 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|