anti-All Species C-MYC antibody for Flow Cytometry

Recommended C-MYC Antibody

Antigen
V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
  • MRTL
  • MYCC
  • bHLHe39
  • c-Myc
  • CMYC
  • c-myc
  • C-MYC
  • LOC100136746
  • RNCMYC
  • mMyc
  • AU016757
  • Myc2
  • Niard
  • Nird
  • MYC
  • cmyc
  • zc-myc
  • c-myc II
  • myc-B
  • myc2
  • CMyc
  • CG10798
  • D-Myc
  • DM
  • DMYc
  • Dm
  • Dmel\CG10798
  • Dmyc
  • EG:BACN5I9.1
  • anon-WO03040301.171
  • bHLHe57
  • c-MYC
  • d-myc
  • dMYC
  • dMyc
  • dMyc1
  • da
  • dm
  • dm/dMyc
  • dm/myc
  • dmyc
  • dmyc1
  • l(1)G0139
  • l(1)G0354
  • l(1)G0359
  • myc
  • MYC proto-oncogene, bHLH transcription factor
  • v-myc myelocytomatosis viral oncogene homolog
  • myelocytomatosis oncogene
  • MYC proto-oncogene, bHLH transcription factor a
  • MYC proto-oncogene, bHLH transcription factor L homeolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
  • CG10798 gene product from transcript CG10798-RB
  • MYC
  • LOC100136746
  • Myc
  • myca
  • myc.L
Epitope
C-Term
149
94
52
50
42
42
24
21
17
16
15
15
14
11
9
9
5
5
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, All Species
662
160
124
48
48
18
16
14
12
12
9
7
6
5
5
4
4
4
3
2
2
2
1
1
1
1
1
1
Host
Mouse
387
272
16
14
7
1
Clonality
Monoclonal
Conjugate
This C-MYC antibody is un-conjugated
40
40
34
17
17
15
9
7
7
7
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
423
199
179
171
157
109
106
63
53
37
19
15
15
14
14
10
3
2
1
Options

Available images

Catalog No. ABIN302092
$ 377.37
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
30.826962 ABIN302017 FACS IHC (p) IP WB FITC Mouse IgG1 C-Term 9E10 15
22.501062 ABIN302064 FACS IHC (p) IP WB HRP Mouse IgG1 C-Term 9E10 15
22.501062 ABIN2749043 FACS IP IHC (p) WB Biotin Mouse IgG1 C-Term 9E10 15
10.257528 ABIN4285695 ELISA FACS ICC IF IP WB Mouse IgG1 Myc-A7 0
-1.7424722 ABIN4285877 ELISA FACS ICC IF WB Alexa Fluor 488 Mouse IgG1 Myc-A7 0
-13.068371 ABIN4285876 ELISA FACS ICC IF WB Alexa Fluor 405 Mouse IgG1 Myc-A7 0
-13.068371 ABIN4285878 ELISA FACS ICC IF WB Alexa Fluor 647 Mouse IgG1 Myc-A7 0
-13.068371 ABIN4285879 ELISA FACS ICC IF WB Alexa Fluor 700 Mouse IgG1 Myc-A7 0
-13.068371 ABIN4285881 ELISA FACS ICC IF WB DyLight 680 Mouse IgG1 Myc-A7 0
-13.068371 ABIN4285882 ELISA FACS ICC IF WB DyLight 488 Mouse IgG1 Myc-A7 0
-13.068371 ABIN4285883 ELISA FACS ICC IF WB DyLight 755 Mouse IgG1 Myc-A7 0
-13.068371 ABIN4285884 ELISA FACS ICC IF WB DyLight 550 Mouse IgG1 Myc-A7 0
-13.068371 ABIN4285885 ELISA FACS ICC IF WB DyLight 350 Mouse IgG1 Myc-A7 0
-13.068371 ABIN4285886 ELISA FACS ICC IF WB DyLight 405 Mouse IgG1 Myc-A7 0
-13.068371 ABIN4257092 FACS APC Mouse IgG1 Myc-A7 0
-13.068371 ABIN4257094 ELISA FACS ICC IF FITC Mouse IgG1 Myc-A7 0
-13.068371 ABIN4257097 FACS PE Mouse IgG1 Myc-A7 0

General

Antigen V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
Epitope C-Term
(149), (94), (52), (50), (42), (42), (24), (21), (17), (16), (15), (15), (14), (11), (9), (9), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, All Species
(662), (160), (124), (48), (48), (18), (16), (14), (12), (12), (9), (7), (6), (5), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(387), (272), (16), (14), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C-MYC antibody is un-conjugated
(40), (40), (34), (17), (17), (15), (9), (7), (7), (7), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(423), (199), (179), (171), (157), (109), (106), (63), (53), (37), (19), (15), (15), (14), (14), (10), (3), (2), (1)
Pubmed 14 references available
Supplier

Product Details anti-C-MYC Antibody

Target Details C-MYC Application Details Handling References for anti-C-MYC antibody (ABIN302092) Images
Specificity The antibody 9E10 can be used to detect the c-Myc tag.
Cross-Reactivity (Details) Human, Recognizes fusion proteins in all species
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Immunogen Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
Clone 9E10
Isotype IgG1
Plasmids, Primers & others

Target Details C-MYC

Product Details anti-C-MYC Antibody Application Details Handling References for anti-C-MYC antibody (ABIN302092) Images back to top
Antigen
Alternative Name c-Myc (MYC Antibody Abstract)
Background The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
Pathways p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-C-MYC Antibody Target Details C-MYC Handling References for anti-C-MYC antibody (ABIN302092) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details References for anti-C-MYC antibody (ABIN302092) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-C-MYC antibody (ABIN302092)

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling Images back to top
Product cited in:

Jelínková, Šafaříková, Vondálová Blanářová, Skender, Hofmanová, Sova, Moyer, Kozubík, Kolář, Ehrmann, Hyršlová Vaculová: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." in: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, 2014

Veracini, Simon, Richard, Schraven, Horejsi, Roche, Benistant: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, 2008

Wang, Campoli, Ko, Luo, Ferrone: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, 2004

Fujiwara, Poikonen, Aleman, Valtavaara, Saksela, Mayer: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, 2002

Baggio, Burgstaller, Hale, Putney, Lane, Lipovsek, Wright, Roberts, Liu, Szostak, Wagner: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, 2002

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, 2002

Hilpert, Hansen, Wessner, Küttner, Welfle, Seifert, Höhne: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, 2001

Siegel, Brandner, Hess: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." in: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, 1999

Background publications

Hoffman, Amanullah, Shafarenko, Liebermann: "The proto-oncogene c-myc in hematopoietic development and leukemogenesis." in: Oncogene, Vol. 21, Issue 21, pp. 3414-21, 2002

Boxer, Dang: "Translocations involving c-myc and c-myc function." in: Oncogene, Vol. 20, Issue 40, pp. 5595-610, 2001

Prendergast: "Mechanisms of apoptosis by c-Myc." in: Oncogene, Vol. 18, Issue 19, pp. 2967-87, 1999

Dang, Resar, Emison, Kim, Li, Prescott, Wonsey, Zeller: "Function of the c-Myc oncogenic transcription factor." in: Experimental cell research, Vol. 253, Issue 1, pp. 63-77, 1999

Spandidos, Pintzas, Kakkanas, Yiagnisis, Mahera, Patra, Agnantis: "Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue." in: Anticancer research, Vol. 7, Issue 6, pp. 1299-304, 1988

Evan, Lewis, Ramsay, Bishop: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986

Images

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling References for anti-C-MYC antibody (ABIN302092) back to top
Supplier Images
 image for anti-V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (C-Term) antibody (ABIN302092) Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Flow Cytometry (FACS) image for anti-V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (C-Term) antibody (ABIN302092) Detection of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfec...