anti-Human C-MYC antibody for Flow Cytometry

Recommended C-MYC Antibody (supplied by: Log in to see )

Antigen
V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
  • MRTL
  • MYCC
  • bHLHe39
  • c-Myc
  • CMYC
  • c-myc
  • C-MYC
  • LOC100136746
  • RNCMYC
  • mMyc
  • AU016757
  • Myc2
  • Niard
  • Nird
  • MYC
  • cmyc
  • zc-myc
  • c-myc II
  • myc-B
  • myc2
  • CMyc
  • CG10798
  • D-Myc
  • DM
  • DMYc
  • Dm
  • Dmel\CG10798
  • Dmyc
  • EG:BACN5I9.1
  • anon-WO03040301.171
  • bHLHe57
  • c-MYC
  • d-myc
  • dMYC
  • dMyc
  • dMyc1
  • da
  • dm
  • dm/dMyc
  • dm/myc
  • dmyc
  • dmyc1
  • l(1)G0139
  • l(1)G0354
  • l(1)G0359
  • myc
  • MYC proto-oncogene, bHLH transcription factor
  • v-myc myelocytomatosis viral oncogene homolog
  • myelocytomatosis oncogene
  • MYC proto-oncogene, bHLH transcription factor a
  • MYC proto-oncogene, bHLH transcription factor L homeolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
  • CG10798 gene product from transcript CG10798-RB
  • MYC
  • LOC100136746
  • Myc
  • myca
  • myc.L
Epitope
C-Term
237
173
74
39
37
36
36
25
21
16
15
15
14
10
9
9
8
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, All Species
815
224
130
48
48
37
35
34
20
19
18
15
14
14
13
9
9
8
6
6
5
4
3
3
2
1
1
1
1
1
Host
Mouse
441
389
30
17
7
1
Clonality
Monoclonal
Conjugate
This C-MYC antibody is conjugated to FITC
48
46
43
26
23
17
16
16
14
12
11
11
11
11
11
11
10
10
9
9
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
513
327
308
222
215
210
137
123
89
63
53
19
16
14
14
10
5
3
3
2
2
1
Options
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Catalog No. ABIN302017
$ 506.03
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.252602 ABIN302092 FACS IHC (p) IP WB Mouse IgG1 C-Term Log in to see 9E10 14
22.252602 ABIN2749043 FACS IP IHC (p) WB Biotin Mouse IgG1 C-Term Log in to see 9E10 15
19 ABIN1497011 FACS IF IHC IHC (p) WB Mouse IgG2b Log in to see 1A6 0
15.559835 ABIN152253 ChIP CyTOF ELISA FACS IHC IHC (fro) IHC (p) IP WB Mouse IgG2a kappa Log in to see 9E11 11
13.252602 ABIN288206 ICC FACS IHC FITC Goat IgG AA 410-419 Log in to see Polyclonal 0
10 ABIN462593 ELISA FACS IF IHC IHC (p) IP WB Mouse IgG1 AA 408-439 Log in to see 9E10 0
10 ABIN2972869 FACS IF/ICC IHC WB Rabbit N-Term Log in to see Polyclonal 0
8.5 ABIN2477752 IHC (fro) FACS IHC (p) ELISA WB Mouse IgG1 Log in to see 9E10 4
5.5 ABIN2477759 FACS FITC Mouse IgG1 Log in to see 9E10 4
5.5 ABIN2477758 FACS IF DyLight 549 Mouse IgG1 Log in to see 9E10 4
5.5 ABIN4369572 FACS IHC (p) IP WB FITC Mouse IgG1 Log in to see 9-00E-10 3
5.5 ABIN536092 FACS IHC (p) IP WB HRP Mouse IgG1 Log in to see 9-00E-10 3
4 ABIN1981415 FACS WB Rabbit IgG AA 40-69 Log in to see Polyclonal 0
4 ABIN2666319 FACS Mouse IgG1, IgG1 kappa Log in to see 9E10 13
4 ABIN6175806 FACS IF IHC (f) APC Mouse IgG1, kappa Log in to see MYC275 0
4 ABIN6175813 FACS IF IHC (f) PerCP Mouse IgG1, kappa Log in to see MYC275 0
4 ABIN6175814 FACS IF IHC (f) PE Mouse IgG1, kappa Log in to see MYC275 0
4 ABIN6175882 FACS IF IHC (f) APC Mouse IgG1, kappa Log in to see MYC909 0
4 ABIN6175890 FACS IF IHC (f) PE Mouse IgG1, kappa Log in to see MYC909 0
4 ABIN6175889 FACS IF IHC (f) PerCP Mouse IgG1, kappa Log in to see MYC909 0

General

Antigen V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
Epitope C-Term
(237), (173), (74), (39), (37), (36), (36), (25), (21), (16), (15), (15), (14), (10), (9), (9), (8), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, All Species
(815), (224), (130), (48), (48), (37), (35), (34), (20), (19), (18), (15), (14), (14), (13), (9), (9), (8), (6), (6), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(441), (389), (30), (17), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C-MYC antibody is conjugated to FITC
(48), (46), (43), (26), (23), (17), (16), (16), (14), (12), (11), (11), (11), (11), (11), (11), (10), (10), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(513), (327), (308), (222), (215), (210), (137), (123), (89), (63), (53), (19), (16), (14), (14), (10), (5), (3), (3), (2), (2), (1)
Pubmed 15 references available
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Product Details anti-C-MYC Antibody

Target Details C-MYC Application Details Handling References for anti-C-MYC antibody (ABIN302017) Images
Specificity The antibody 9E10 may be used to detect the c-Myc tag.
The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
Characteristics The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Immunogen Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
Clone 9E10
Isotype IgG1
Plasmids, Primers & others

Target Details C-MYC

Product Details anti-C-MYC Antibody Application Details Handling References for anti-C-MYC antibody (ABIN302017) Images back to top
Antigen
Alternative Name c-Myc (MYC Antibody Abstract)
Pathways p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-C-MYC Antibody Target Details C-MYC Handling References for anti-C-MYC antibody (ABIN302017) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis.Suggested working dilution is 1:200. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator. Application note: Membrane permeabilization is required.
Restrictions For Research Use only

Handling

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details References for anti-C-MYC antibody (ABIN302017) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-C-MYC antibody (ABIN302017)

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling Images back to top
Product cited in:

Jelínková, Šafaříková, Vondálová Blanářová, Skender, Hofmanová, Sova, Moyer, Kozubík, Kolář, Ehrmann, Hyršlová Vaculová: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." in: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, 2014

Veracini, Simon, Richard, Schraven, Horejsi, Roche, Benistant: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, 2008

Wang, Campoli, Ko, Luo, Ferrone: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, 2004

Fujiwara, Poikonen, Aleman, Valtavaara, Saksela, Mayer: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, 2002

Baggio, Burgstaller, Hale, Putney, Lane, Lipovsek, Wright, Roberts, Liu, Szostak, Wagner: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, 2002

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, 2002

Hilpert, Hansen, Wessner, Küttner, Welfle, Seifert, Höhne: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, 2001

Siegel, Brandner, Hess: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." in: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, 1999

Background publications

Hoffman, Amanullah, Shafarenko, Liebermann: "The proto-oncogene c-myc in hematopoietic development and leukemogenesis." in: Oncogene, Vol. 21, Issue 21, pp. 3414-21, 2002

Boxer, Dang: "Translocations involving c-myc and c-myc function." in: Oncogene, Vol. 20, Issue 40, pp. 5595-610, 2001

Prendergast: "Mechanisms of apoptosis by c-Myc." in: Oncogene, Vol. 18, Issue 19, pp. 2967-87, 1999

Dang, Resar, Emison, Kim, Li, Prescott, Wonsey, Zeller: "Function of the c-Myc oncogenic transcription factor." in: Experimental cell research, Vol. 253, Issue 1, pp. 63-77, 1999

Spandidos, Pintzas, Kakkanas, Yiagnisis, Mahera, Patra, Agnantis: "Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue." in: Anticancer research, Vol. 7, Issue 6, pp. 1299-304, 1988

Evan, Lewis, Ramsay, Bishop: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986

Persson, Hennighausen, Taub, DeGrado, Leder: "Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation." in: Science (New York, N.Y.), Vol. 225, Issue 4663, pp. 687-93, 1984

Images

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling References for anti-C-MYC antibody (ABIN302017) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (C-Term) antibody (FITC) (ABIN302017) Detection of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfec...