anti-Human C-MYC antibody for Simple Western

Recommended C-MYC Antibody (supplied by: Log in to see )

Antigen
V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
  • MRTL
  • MYCC
  • bHLHe39
  • c-Myc
  • CMYC
  • c-myc
  • C-MYC
  • LOC100136746
  • RNCMYC
  • mMyc
  • AU016757
  • Myc2
  • Niard
  • Nird
  • MYC
  • cmyc
  • zc-myc
  • c-myc II
  • myc-B
  • myc2
  • CMyc
  • CG10798
  • D-Myc
  • DM
  • DMYc
  • Dm
  • Dmel\CG10798
  • Dmyc
  • EG:BACN5I9.1
  • anon-WO03040301.171
  • bHLHe57
  • c-MYC
  • d-myc
  • dMYC
  • dMyc
  • dMyc1
  • da
  • dm
  • dm/dMyc
  • dm/myc
  • dmyc
  • dmyc1
  • l(1)G0139
  • l(1)G0354
  • l(1)G0359
  • myc
  • MYC proto-oncogene, bHLH transcription factor
  • v-myc myelocytomatosis viral oncogene homolog
  • myelocytomatosis oncogene
  • MYC proto-oncogene, bHLH transcription factor a
  • MYC proto-oncogene, bHLH transcription factor L homeolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
  • CG10798 gene product from transcript CG10798-RB
  • MYC
  • LOC100136746
  • Myc
  • myca
  • myc.L
Epitope
AA 408-439, AA 410-419, C-Term
242
178
75
38
37
36
36
25
21
16
15
15
14
10
10
9
9
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
837
225
129
46
46
45
40
36
20
20
19
15
14
14
12
9
9
8
6
6
5
4
3
3
2
1
1
1
1
1
Host
Mouse
474
387
30
16
8
1
Clonality
Monoclonal
Conjugate
This C-MYC antibody is un-conjugated
51
48
48
28
25
19
17
17
17
14
13
13
13
13
13
13
12
12
9
9
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes)
527
331
328
240
229
216
155
128
94
63
52
18
16
14
14
10
6
6
2
2
2
1
Supplier
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Catalog No. ABIN4285807
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4285818 CyTOF FACS IF IHC (p) SimWes Mouse IgG1 kappa AA 408-439, AA 410-419, C-Term Log in to see 9E10-3 0
1 ABIN4285693 ChIP ELISA ICC IF IHC IHC (p) IP SimWes WB Rabbit AA 385-435 Log in to see Polyclonal 0

General

Antigen V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
Epitope AA 408-439, AA 410-419, C-Term
(242), (178), (75), (38), (37), (36), (36), (25), (21), (16), (15), (15), (14), (10), (10), (9), (9), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(837), (225), (129), (46), (46), (45), (40), (36), (20), (20), (19), (15), (14), (14), (12), (9), (9), (8), (6), (6), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(474), (387), (30), (16), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C-MYC antibody is un-conjugated
(51), (48), (48), (28), (25), (19), (17), (17), (17), (14), (13), (13), (13), (13), (13), (13), (12), (12), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes)
(527), (331), (328), (240), (229), (216), (155), (128), (94), (63), (52), (18), (16), (14), (14), (10), (6), (6), (2), (2), (2), (1)
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Product Details anti-C-MYC Antibody

Target Details C-MYC Application Details Handling Images
Specificity It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease
No Cross-Reactivity Mouse (Murine), Chicken, Rat (Rattus)
Purification Protein A or G purified
Immunogen A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.
Clone 9E10-3
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details C-MYC

Product Details anti-C-MYC Antibody Application Details Handling Images back to top
Antigen
Alternative Name C-Myc (MYC Antibody Abstract)
Background Gene Symbol: MYC
Gene ID 4609
UniProt P01106
Pathways p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-C-MYC Antibody Target Details C-MYC Handling Images back to top
Application Notes Simple Western 50 μg/mL, Flow Cytometry 0.5 - 1 μg/million cells in 0.1 ml, Immunohistochemistry-Paraffin 1 - 2 μg/mL, Immunofluorescence 1 - 2 μg/mLHu-chromosome location: 8q24.2 Molecular weight of antigen: 62-64 kDa Immunohistochemistry-Paraffin 1 - 2 μg/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. . In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Images back to top
Format Liquid
Buffer 10 mM PBS and 0.05 % BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.