anti-Monkey C-MYC antibody for Immunoprecipitation

Recommended C-MYC Antibody (supplied by: Log in to see )

Antigen
V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
  • MRTL
  • MYCC
  • bHLHe39
  • c-Myc
  • CMYC
  • c-myc
  • C-MYC
  • LOC100136746
  • RNCMYC
  • mMyc
  • AU016757
  • Myc2
  • Niard
  • Nird
  • MYC
  • cmyc
  • zc-myc
  • c-myc II
  • myc-B
  • myc2
  • CMyc
  • CG10798
  • D-Myc
  • DM
  • DMYc
  • Dm
  • Dmel\CG10798
  • Dmyc
  • EG:BACN5I9.1
  • anon-WO03040301.171
  • bHLHe57
  • c-MYC
  • d-myc
  • dMYC
  • dMyc
  • dMyc1
  • da
  • dm
  • dm/dMyc
  • dm/myc
  • dmyc
  • dmyc1
  • l(1)G0139
  • l(1)G0354
  • l(1)G0359
  • myc
  • MYC proto-oncogene, bHLH transcription factor
  • v-myc myelocytomatosis viral oncogene homolog
  • myelocytomatosis oncogene
  • MYC proto-oncogene, bHLH transcription factor a
  • MYC proto-oncogene, bHLH transcription factor L homeolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
  • CG10798 gene product from transcript CG10798-RB
  • MYC
  • LOC100136746
  • Myc
  • myca
  • myc.L
Epitope
AA 200-250, N-Term
167
121
77
46
43
29
26
18
18
15
15
15
14
10
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Human, Monkey
705
215
125
50
42
36
20
19
19
14
14
13
12
9
9
6
5
5
4
4
4
3
3
2
1
1
1
1
1
Host
Rabbit
377
342
29
16
8
1
Clonality
Polyclonal
Conjugate
This C-MYC antibody is un-conjugated
47
41
40
21
18
17
17
14
13
13
13
13
13
13
12
12
12
10
5
5
5
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
484
246
230
228
228
162
151
122
92
52
19
14
14
11
10
8
6
6
4
2
2
1
Supplier
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Catalog No. ABIN2747734
$ 485.57
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4994828 IHC IHC (p) IP WB HRP Mouse IgG1 Log in to see PL14 6
1 ABIN4957393 ICC IF IP WB Mouse IgG1 Log in to see PL14 0
1 ABIN2749440 IP ELISA WB FITC Rabbit IgG AA 200-250, N-Term Log in to see Polyclonal 0
1 ABIN2749441 IP ELISA WB FITC Rabbit IgG AA 400-454, C-Term Log in to see Polyclonal 0
1 ABIN2749439 IP ELISA WB Rabbit IgG AA 400-454, C-Term Log in to see Polyclonal 0
1 ABIN5558216 ELISA IP WB Biotin Rabbit N-Term Log in to see Polyclonal 0
1 ABIN5558217 ELISA IP WB Biotin Rabbit C-Term Log in to see Polyclonal 0

General

Antigen V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
Epitope AA 200-250, N-Term
(167), (121), (77), (46), (43), (29), (26), (18), (18), (15), (15), (15), (14), (10), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Human, Monkey
(705), (215), (125), (50), (42), (36), (20), (19), (19), (14), (14), (13), (12), (9), (9), (6), (5), (5), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(377), (342), (29), (16), (8), (1)
Clonality
Polyclonal
Conjugate This C-MYC antibody is un-conjugated
(47), (41), (40), (21), (18), (17), (17), (14), (13), (13), (13), (13), (13), (13), (12), (12), (12), (10), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(484), (246), (230), (228), (228), (162), (151), (122), (92), (52), (19), (14), (14), (11), (10), (8), (6), (6), (4), (2), (2), (1)
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Product Details anti-C-MYC Antibody

Target Details C-MYC Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN2749442
Cross-Reactivity Rat (Rattus), Mouse (Murine)
Cross-Reactivity (Details) The antibody does not cross react to any other cellular protein.
Characteristics cMyc selective antibodies were generated against a peptide taken from the human protein. The cMyc-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The cMyc-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides cMyc Western blot positive control samples in SDS-PAGE sample buffer.
Purification Affinity Purified
Immunogen Synthetic peptide taken within amino acid region 200-250 on human cMyc protein.
Isotype IgG

Target Details C-MYC

Product Details anti-C-MYC Antibody Application Details Handling Images back to top
Antigen
Alternative Name C-Myc (MYC Antibody Abstract)
Background Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) which are transcription factors implicated in cellular proliferation, cellular transformation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein acts as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Myc proteins are nuclear proteins with relatively short half-lives and play a central role as both a key target and a cooperating transcription factor to drive oncogenic growth and is up-regulated in several types of cancers. The cMyc protein is a 55 kDa nuclear factor ubiquitously expressed in the nucleus and is the prototype for oncogene activation by chromosomal translocation. Mutations, overexpression, rearrangement and translocation of this gene are associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt's lymphoma. In Burkitt's lymphomas, the removal or specific mutation of exon 1 in cMyc translocations correlates with suppression of synthesis of the larger protein, and thus contributes to the oncogenic activation of c-myc. cMyc activates the transcription of growth-related genes and its overexpression induces cell-cycle progression thereby implicating in a variety of cancers. Amplification of the c-Myc gene is found in several types of human tumors including lung, breast and colon carcinoma. C-Myc is modified by glycosylation and phosphorylation, and interacts with a number of proteins including SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, and Sp1. cMyc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer. c-Myc participates in gene transcription regulation and binds DNA in a non-specific manner, specifically recognizing core sequence 5'-CAC[GA]TG-3'. The gene for cMyc is present on chromosome 8q24.21.
NCBI Accession NP_002458
UniProt P01106
Pathways p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-C-MYC Antibody Target Details C-MYC Handling Images back to top
Application Notes Antibodies were tested in ELISA and western blotting applications at 1:500 dilution using ABIN1686561 samples. Antibody dilutions for these antibodies are for reference only, investigators are expected to determine the optimal conditions. Application of this antibody in other protocols has not yet tested.
WB: > 1:500
IMM & IP pull-down assays: n.d.
IHC: n.d.
Investigators using this antibody in protocols other than listed above can request a complimentary sample of this antibody. n.d. not necessarily means the antibody is not suitable for that application, it simply means we have not yet characterized the antibody for that application.
The antibody labels a strong band of cMyc at 55 kDa in ABIN1686561 samples and in other cancer cell lines.
Restrictions For Research Use only

Handling

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Images back to top
Format Liquid
Concentration 0.55-0.75 μg/μL
Storage -20 °C
Storage Comment Storage of very dilute antibody solutions is not recommended.