anti-Human CDKN1A antibody for Enzyme Immunoassay

Recommended CDKN1A Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
  • p21
  • cip1
  • p16Xic2
  • CCND1
  • sdi1
  • waf1
  • cap20
  • cdkn1
  • mda-6
  • p21cip1
  • CDKN1A
  • si:ch1073-48m5.2
  • Cip1
  • UV96
  • Waf1
  • CAP20
  • CDKN1
  • CIP1
  • MDA-6
  • P21
  • SDI1
  • WAF1
  • p21CIP1
  • CDKI
  • Cdkn1
  • mda6
  • p21Cip1
  • p21WAF
  • cyclin-dependent kinase inhibitor 1A L homeolog
  • cyclin-dependent kinase inhibitor 1
  • cyclin D1
  • cyclin-dependent kinase inhibitor 1A
  • cyclin dependent kinase inhibitor 1A
  • cyclin-dependent kinase inhibitor 1A (P21)
  • cdkn1a.L
  • cki1
  • CCND1
  • cdkn1a
  • CDKN1A
  • Cdkn1a
Epitope
pThr145
49
40
39
27
20
20
18
18
18
18
15
15
15
15
12
9
8
7
7
5
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human
575
182
122
30
23
9
6
5
4
2
1
1
1
1
1
1
1
1
Host
Rabbit
309
307
2
1
Clonality
Polyclonal
Conjugate
This CDKN1A antibody is un-conjugated
33
32
32
23
23
16
16
15
12
12
10
10
10
10
10
10
10
8
6
6
6
6
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
353
221
186
166
148
140
67
60
59
53
30
14
6
6
6
2
1
1
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Catalog No. ABIN358181
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358182 EIA IHC (p) WB Rabbit Ig Fraction pThr57 Log in to see Polyclonal 5
1 ABIN358179 DB EIA Rabbit Ig Fraction pSer130 Log in to see Polyclonal 5
1 ABIN359673 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 5
1 ABIN271762 EIA WB Rabbit Log in to see Polyclonal
1 ABIN318089 EIA IHC (p) WB Rabbit pThr145 Log in to see Polyclonal
1 ABIN2718408 EIA WB Rabbit IgG Log in to see Polyclonal

General

Antigen Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
Epitope pThr145
(49), (40), (39), (27), (20), (20), (18), (18), (18), (18), (15), (15), (15), (15), (12), (9), (8), (7), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(575), (182), (122), (30), (23), (9), (6), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(309), (307), (2), (1)
Clonality
Polyclonal
Conjugate This CDKN1A antibody is un-conjugated
(33), (32), (32), (23), (23), (16), (16), (15), (12), (12), (10), (10), (10), (10), (10), (10), (10), (8), (6), (6), (6), (6), (4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(353), (221), (186), (166), (148), (140), (67), (60), (59), (53), (30), (14), (6), (6), (6), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-CDKN1A Antibody

Target Details CDKN1A Application Details Handling References for anti-CDKN1A antibody (ABIN358181) Images
Specificity This antibody detects p21Cip1 pThr145.
Purification Protein A affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T145 of human P21CIP1.
Isotype Ig Fraction

Target Details CDKN1A

Product Details anti-CDKN1A Antibody Application Details Handling References for anti-CDKN1A antibody (ABIN358181) Images back to top
Antigen
Alternative Name CDKN1A / p21WAF1 (CDKN1A Antibody Abstract)
Background P21CIP1 is a potent cyclin-dependent kinase inhibitor. This protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21CIP1 can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein has been reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1
Molecular Weight 17988 Da
Gene ID 1026, 9606
UniProt P38936
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1A Antibody Target Details CDKN1A Handling References for anti-CDKN1A antibody (ABIN358181) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details References for anti-CDKN1A antibody (ABIN358181) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CDKN1A antibody (ABIN358181)

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top
Background publications

Marqués, Kumar, Poveda, Zuluaga, Hernández, Jackson, Pasero, Carrera: "Specific function of phosphoinositide 3-kinase beta in the control of DNA replication." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 18, pp. 7525-30, 2009

Images

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling References for anti-CDKN1A antibody (ABIN358181) back to top
Supplier Images
 image for anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr145) antibody (ABIN358181) anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr145) antibody
 image for anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr145) antibody (ABIN358181) anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr145) antibody (Image 2)