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|Antigen||Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies|
|Conjugate||This CDKN1A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
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Product Details anti-CDKN1A AntibodyTarget Details CDKN1A Application Details Handling References for anti-CDKN1A antibody (ABIN358181) Images
|Specificity||This antibody detects p21Cip1 pThr145.|
|Purification||Protein A affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T145 of human P21CIP1.|
Target Details CDKN1AProduct Details anti-CDKN1A Antibody Application Details Handling References for anti-CDKN1A antibody (ABIN358181) Images back to top
|Alternative Name||CDKN1A / p21WAF1 (CDKN1A Antibody Abstract)|
|Background||P21CIP1 is a potent cyclin-dependent kinase inhibitor. This protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21CIP1 can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein has been reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1|
|Molecular Weight||17988 Da|
|Gene ID||1026, 9606|
|Pathways||p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy|
Application DetailsProduct Details anti-CDKN1A Antibody Target Details CDKN1A Handling References for anti-CDKN1A antibody (ABIN358181) Images back to top
ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CDKN1A Antibody Target Details CDKN1A Application Details References for anti-CDKN1A antibody (ABIN358181) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-CDKN1A antibody (ABIN358181)Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top
Marqués, Kumar, Poveda, Zuluaga, Hernández, Jackson, Pasero, Carrera: "Specific function of phosphoinositide 3-kinase beta in the control of DNA replication." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 18, pp. 7525-30, 2009
ImagesProduct Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling References for anti-CDKN1A antibody (ABIN358181) back to top