anti-Mouse (Murine) CDKN1A antibody for Flow Cytometry
Recommended CDKN1A Antibody (supplied by: Log in to see )
- Antigen
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Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
show synonyms for this antigen- p21
- cip1
- p16Xic2
- CCND1
- sdi1
- waf1
- cap20
- cdkn1
- mda-6
- p21cip1
- CDKN1A
- si:ch1073-48m5.2
- Cip1
- UV96
- Waf1
- CAP20
- CDKN1
- CIP1
- MDA-6
- P21
- SDI1
- WAF1
- p21CIP1
- CDKI
- Cdkn1
- mda6
- p21Cip1
- p21WAF
- cyclin-dependent kinase inhibitor 1A L homeolog
- cyclin-dependent kinase inhibitor 1
- cyclin D1
- cyclin-dependent kinase inhibitor 1A
- cyclin dependent kinase inhibitor 1A
- cyclin-dependent kinase inhibitor 1A (P21)
- cdkn1a.L
- cki1
- CCND1
- cdkn1a
- CDKN1A
- Cdkn1a
- Reactivity
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Human, Mouse (Murine), Primate, Rat (Rattus)
Alternatives - Host
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Mouse
Alternatives - Clonality
- Conjugate
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This CDKN1A antibody is un-conjugated
Alternatives - Application
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Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives - Supplier
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- Supplier Product No.
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | ABIN4342330 | FACS ICC IF IHC IHC (fro) IHC (p) WB | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | |||
| 1 | ABIN4342329 | FACS ICC IF IHC IHC (fro) IHC (p) WB | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | |||
| 1 | ABIN4342362 | FACS IF IHC (p) | Mouse | IgG1 kappa | Log in to see | HJ21 | 0 | |||
| 1 | ABIN4342363 | FACS IF IHC (p) | Mouse | IgG1 kappa | Log in to see | HJ21 | 0 | |||
| 1 | ABIN4342364 | FACS IF IHC (p) | Mouse | IgG1 kappa | Log in to see | HJ21 | 0 | |||
| 1 | ABIN4342338 | FACS | APC | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342340 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | DyLight 650 | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342344 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | DyLight 755 | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342347 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | DyLight 550 | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342349 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | DyLight 405 | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342348 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | DyLight 350 | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342339 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | Biotin | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342335 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | Alexa Fluor 488 | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342334 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | Alexa Fluor 405 | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342346 | FACS | PE | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342341 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | DyLight 680 | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342342 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | DyLight 488 | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342345 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | PerCP | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342337 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | Alexa Fluor 700 | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 | ||
| 1 | ABIN4342336 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB | Alexa Fluor 647 | Mouse | IgG1 kappa | Log in to see | WA-1 (HJ21) | 0 |
General |
|
|---|---|
| Antigen | Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies |
| Reactivity | Human, Mouse (Murine), Primate, Rat (Rattus) Alternatives |
| Host | Mouse Alternatives |
| Clonality (Clone) | |
| Conjugate | This CDKN1A antibody is un-conjugated Alternatives |
| Application |
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives
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11 references available |
| Supplier | Log in to see |
Product Details anti-CDKN1A AntibodyTarget Details CDKN1A Application Details Handling References for anti-CDKN1A antibody (ABIN4342331) Images |
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| Specificity | This MAb recognizes a 21 kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, MAb WA-1 is useful either as a solid phase or for detection of p21 protein. |
| Purification | Protein A or G purified |
| Immunogen | Recombinant human p21 protein was used as immunogen to generate the antibody. |
| Clone | WA-1 (HJ21) |
| Isotype | IgG1 kappa |
Target Details CDKN1AProduct Details anti-CDKN1A Antibody Application Details Handling References for anti-CDKN1A antibody (ABIN4342331) Images back to top |
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| Antigen | |
| Alternative Name | p21/CIP1/CDKN1A (CDKN1A Antibody Abstract) |
| Background | Gene Symbol: CDKN1A |
| Molecular Weight | Theoretical MW: 21 kDa |
| Gene ID | 1026 |
| UniProt | P38936 |
| Pathways | p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy |
Application DetailsProduct Details anti-CDKN1A Antibody Target Details CDKN1A Handling References for anti-CDKN1A antibody (ABIN4342331) Images back to top |
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| Application Notes | Western Blot, Flow Cytometry 0.5-1 μg/million cells, Immunohistochemistry 0.5-1 μg/mL, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mLHuman Chromosome Location: 6p21.31 Use in ELISA has been reported in literature. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Western blot reported in scientific literature (PMID 28137862). |
| Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
| Restrictions | For Research Use only |
HandlingProduct Details anti-CDKN1A Antibody Target Details CDKN1A Application Details References for anti-CDKN1A antibody (ABIN4342331) Images back to top |
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| Format | Liquid |
| Buffer |
10 mM PBS and 0.05 % BSA Buffer contains: 0.05 % Sodium Azide |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Storage | 4 °C |
| Storage Comment | Store at 4°C. |
References for anti-CDKN1A antibody (ABIN4342331)Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top |
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| Product cited in: |
Xiang, Yang, Xin, Gaertig, Reeves, Li, Li: "DYRK1A regulates Hap1-Dcaf7/WDR68 binding with implication for delayed growth in Down syndrome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 7, pp. E1224-E1233, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)). Huang, Huang, Gou, Wang, Liao, Wang: "Platinum(IV) complexes conjugated with phenstatin analogue as inhibitors of microtubule polymerization and reverser of multidrug resistance." in: Bioorganic & medicinal chemistry, Vol. 25, Issue 17, pp. 4686-4700, 2017 (Sample species: Human). Ge, Pettan-Brewer, Morton, Carter, Fatemi, Rabinovitch, Ladiges: "Mitochondrial catalase suppresses naturally occurring lung cancer in old mice." in: Pathobiology of aging & age related diseases, Vol. 5, pp. 28776, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry Romanov, Pospelov, Pospelova: "Cyclin-dependent kinase inhibitor p21(Waf1): contemporary view on its role in senescence and oncogenesis." in: Biochemistry. Biokhimii͡a, Vol. 77, Issue 6, pp. 575-84, 2012 Koike, Yutoku, Koike: "Accumulation of p21 proteins at DNA damage sites independent of p53 and core NHEJ factors following irradiation." in: Biochemical and biophysical research communications, Vol. 412, Issue 1, pp. 39-43, 2011 Nobre, Herráez-Hernández, Fei, Langbein, Kaden, Gröne, de Villiers: "E7 oncoprotein of novel human papillomavirus type 108 lacking the E6 gene induces dysplasia in organotypic keratinocyte cultures." in: Journal of virology, Vol. 83, Issue 7, pp. 2907-16, 2009 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections) Rossner, Binkova, Milcova, Solansky, Zidzik, Lyubomirova, Farmer, Sram: "Air pollution by carcinogenic PAHs and plasma levels of p53 and p21(WAF1) proteins." in: Mutation research, Vol. 620, Issue 1-2, pp. 34-40, 2007 (Sample species: Human). Rössner, Binková, Chvátalová, Srám: "Acrylonitrile exposure: the effect on p53 and p21(WAF1) protein levels in the blood plasma of occupationally exposed workers and in vitro in human diploid lung fibroblasts." in: Mutation research, Vol. 517, Issue 1-2, pp. 239-50, 2002 Further details: Western Blotting Scott, Ingram, Ball: "PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein." in: The EMBO journal, Vol. 21, Issue 24, pp. 6771-80, 2002 Further details: Western Blotting Blaydes, Luciani, Pospisilova, Ball, Vojtesek, Hupp: "Stoichiometric phosphorylation of human p53 at Ser315 stimulates p53-dependent transcription." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 4699-708, 2001 Further details: Western Blotting Uy, White, Oka: "Observation of Delta|k - l | = 3 Transitions in the nu3 Band of H3 O+" in: Journal of molecular spectroscopy, Vol. 183, Issue 2, pp. 240-4, 1997 Further details: Western Blotting |
ImagesProduct Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling References for anti-CDKN1A antibody (ABIN4342331) back to top |
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| Supplier Images |
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - ...
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