anti-Human CHEK1 antibody for Immunofluorescence

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CAMK/CAMKL/CHK1 protein kinase Chk1
  • checkpoint kinase 1 S homeolog
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1.S
  • Chek1
  • chek1
Epitope
AA 250-300
68
64
38
33
16
16
15
15
14
12
6
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
474
191
163
14
14
10
10
10
7
5
4
4
4
2
1
1
Host
Rabbit
411
53
23
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Proximity Ligation Assay (PLA), Western Blotting (WB)
407
180
140
73
67
40
24
21
19
10
7
6
6
5
Supplier
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Catalog No. ABIN151911
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.668406 ABIN2705869 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
11.668406 ABIN2705868 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
11.668406 ABIN2705867 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
11.668406 ABIN2957528 IC IF WB Rabbit pSer301 Log in to see Polyclonal 0
11.668406 ABIN1532570 IF ELISA WB Rabbit IgG AA 256-305 Log in to see Polyclonal 2
11.668406 ABIN1531554 IF ELISA WB Rabbit IgG AA 256-305, pSer286 Log in to see Polyclonal 2
11.668406 ABIN1531555 IF ELISA WB Rabbit IgG AA 271-320, pSer301 Log in to see Polyclonal 2
11.668406 ABIN1532571 IF ELISA Rabbit IgG AA 271-320 Log in to see Polyclonal 1
11.668406 ABIN151295 ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 2G1D5 0
11.668406 ABIN2857048 ICC IF IHC (p) WB Rabbit IgG Internal Region, pSer345 Log in to see Polyclonal 0
11.668406 ABIN2857050 ICC IF IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
11.668406 ABIN2857049 ICC IF WB Rabbit IgG Internal Region, Ser345 Log in to see Polyclonal 0
11.668406 ABIN1870069 IF IHC WB Rabbit IgG pSer280 Log in to see Polyclonal 0
11.668406 ABIN1870071 IF WB Rabbit IgG pSer317 Log in to see Polyclonal 0
11.668406 ABIN514379 IF ELISA Mouse IgG2b kappa AA 361-476, partial Log in to see 2G3 0
11.668406 ABIN5075918 ICC IF Rabbit IgG Log in to see Polyclonal 0
1 ABIN3197993 IC IF IHC WB Rabbit pSer345 Log in to see Polyclonal 0
1 ABIN407619 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN3182434 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal 0
1 ABIN3183900 ELISA IF WB Rabbit IgG Ser2481 Log in to see Polyclonal 0

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope AA 250-300
(68), (64), (38), (33), (16), (16), (15), (15), (14), (12), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(474), (191), (163), (14), (14), (10), (10), (10), (7), (5), (4), (4), (4), (2), (1), (1)
Host Rabbit
(411), (53), (23), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Proximity Ligation Assay (PLA), Western Blotting (WB)
(407), (180), (140), (73), (67), (40), (24), (21), (19), (10), (7), (6), (6), (5)
Pubmed 6 references available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN151911) Images
Purification Immunogen affinity purified
Immunogen The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in SwissProt entry O14757 (GeneID 1111).
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 antibody (ABIN151911) Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Gene Symbol: CHEK1
Molecular Weight Theoretical MW: 54 kDa
Gene ID 1111
UniProt O14757
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 antibody (ABIN151911) Images back to top
Application Notes Western Blot 1:10000-1:25000, Immunohistochemistry 1:1000-1:5000, Immunocytochemistry/Immunofluorescence 1:500-1:2000, Immunohistochemistry-Paraffin 1:1000-1:5000, Proximity Ligation Assay 1:1000-1:10000The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 antibody (ABIN151911) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-CHEK1 antibody (ABIN151911)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Product cited in:

Jazaeri, Shibata, Park, Bryant, Conaway, Modesitt, Smith, Milhollen, Berger, Dutta: "Overcoming platinum resistance in preclinical models of ovarian cancer using the neddylation inhibitor MLN4924." in: Molecular cancer therapeutics, Vol. 12, Issue 10, pp. 1958-67, 2013 (Sample species: Human). Further details: Western Blotting

Terai, Shibata, Abbas, Dutta: "Degradation of p12 subunit by CRL4Cdt2 E3 ligase inhibits fork progression after DNA damage." in: The Journal of biological chemistry, Vol. 288, Issue 42, pp. 30509-14, 2013 (Sample species: Human). Further details: Western Blotting

Hong, Hu, Yuan, Sang, Bu, Chen, Yang, Li, Huang, Chen, Liang, Zhang, Pan, Zeng, Kang: "CHK1 targets spleen tyrosine kinase (L) for proteolysis in hepatocellular carcinoma." in: The Journal of clinical investigation, Vol. 122, Issue 6, pp. 2165-75, 2012 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry

Karnani, Dutta: "The effect of the intra-S-phase checkpoint on origins of replication in human cells." in: Genes & development, Vol. 25, Issue 6, pp. 621-33, 2011

Shiotani, Kobayashi, Watanabe, Yamamoto, Sugimura, Wakabayashi: "Involvement of the ATR- and ATM-dependent checkpoint responses in cell cycle arrest evoked by pierisin-1." in: Molecular cancer research : MCR, Vol. 4, Issue 2, pp. 125-33, 2006

Bai, Wang, Huo, Xie, Xie, Xu, Wang: "Serine/Threonine Kinase CHEK1-Dependent Transcriptional Regulation of RAD54L Promotes Proliferation and Radio Resistance in Glioblastoma." in: Translational oncology, Vol. 11, Issue 1, pp. 140-146, 1970 (Sample species: Mouse (Murine)).

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN151911) back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 250-300) antibody (ABIN151911) Detection of Chk1 in HeLa whole cell lysates using ABIN151911.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 250-300) antibody (ABIN151911) Immunohistochemistry-Paraffin: Chk1 Antibody [ABIN151911] - Human testicular seminoma...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 250-300) antibody (ABIN151911) Western Blot: Chk1 Antibody [ABIN151911] - Whole cell lysate (20 ug) from HeLa cells....
Proximity Ligation Assay (PLA) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 250-300) antibody (ABIN151911) Proximity Ligation Assay: Chk1 Antibody [ABIN151911] - Secondary-conjugate Duolink II...
Proximity Ligation Assay (PLA) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 250-300) antibody (ABIN151911) Proximity Ligation Assay: Chk1 Antibody [ABIN151911] - Secondary-conjugate Duolink In...
Proximity Ligation Assay (PLA) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 250-300) antibody (ABIN151911) Proximity Ligation Assay: Chk1 Antibody [ABIN151911] - Secondary-conjugate Duolink In...
Proximity Ligation Assay (PLA) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 250-300) antibody (ABIN151911) Proximity Ligation Assay: Chk1 Antibody [ABIN151911] - Secondary-conjugate Duolink II...
Proximity Ligation Assay (PLA) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 250-300) antibody (ABIN151911) Proximity Ligation Assay: Chk1 Antibody [ABIN151911] - Secondary-conjugate Duolink in...
Immunofluorescence (IF) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 250-300) antibody (ABIN151911) Immunocytochemistry/Immunofluorescence: Chk1 Antibody [ABIN151911] - NBF-fixed HeLa c...
Immunocytochemistry (ICC) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 250-300) antibody (ABIN151911) Immunohistochemistry: Chk1 Antibody - Sample: FFPE section of human breast carcinoma...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 250-300) antibody (ABIN151911) Western Blot: Chk1 Antibody - Detection of Human Chk1 by Western Blot. Samples: Whol...