anti-Human CHEK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CAMK/CAMKL/CHK1 protein kinase Chk1
  • checkpoint kinase 1 S homeolog
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1.S
  • Chek1
  • chek1
Epitope
Internal Region, pSer345
73
57
40
35
28
17
16
15
14
12
7
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
503
210
179
14
14
10
10
10
7
5
4
4
4
2
1
1
Host
Rabbit
445
51
22
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
14
13
12
11
10
8
3
3
3
3
3
3
3
3
3
3
3
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
434
216
171
80
68
40
32
21
18
16
13
7
6
6
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2857048
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.576218 ABIN2857050 ICC IF IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
14.5 ABIN389543 DB IHC (p) WB Rabbit Ig Fraction pSer317 Log in to see Polyclonal 4
11.5 ABIN757072 IF (p) IHC (p) WB Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal 1
11.119598 ABIN214737 IHC IHC (p) WB Rabbit AA 192-241 Log in to see Polyclonal 0
11.119598 ABIN498255 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
10 ABIN487487 IHC (p) IP WB Mouse IgG2b Log in to see DCS-310 0
8.119598 ABIN498827 IHC (p) Rabbit pSer345 Log in to see Polyclonal 0
8.119598 ABIN498915 IHC (p) Rabbit Log in to see Polyclonal 0
8.119598 ABIN498828 IHC (p) Rabbit pSer317 Log in to see Polyclonal 0
8.119598 ABIN498916 IHC (p) Rabbit Log in to see Polyclonal 0
7 ABIN358108 EIA IHC (p) WB Rabbit Ig Fraction pSer280 Log in to see Polyclonal 5
7 ABIN358109 EIA IHC (p) WB Rabbit Ig Fraction pSer317 Log in to see Polyclonal 5
7 ABIN389542 IHC (p) WB Rabbit Ig Fraction pSer280 Log in to see Polyclonal 3
7 ABIN197292 IHC (p) WB Rabbit Ser317 Log in to see Polyclonal 2
7 ABIN2888962 ELISA IHC IHC (p) WB Rabbit IgG AA 286-335 Log in to see Polyclonal 0
7 ABIN1959863 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN265347 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal 0
7 ABIN5575397 IHC (p) WB Rabbit pSer296 Log in to see Polyclonal 0
7 ABIN5575400 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
7 ABIN5575403 IHC (p) WB Rabbit Log in to see Polyclonal 0

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope Internal Region, pSer345
(73), (57), (40), (35), (28), (17), (16), (15), (14), (12), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(503), (210), (179), (14), (14), (10), (10), (10), (7), (5), (4), (4), (4), (2), (1), (1)
Host Rabbit
(445), (51), (22), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(14), (13), (12), (11), (10), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(434), (216), (171), (80), (68), (40), (32), (21), (18), (16), (13), (7), (6), (6)
Supplier Log in to see

Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling Images
Cross-Reactivity Cow (Bovine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
Cross-Reactivity (Details) Rat (100 %), Zebrafish (88 %), Xenopus laevis (88 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to Chk1 (phospho Ser345) (CHK1 checkpoint homolog (S. pombe))
Chk1 (phospho Ser345) antibody [C1C2], Internal
Purification Purified by antigen-affinity chromatography.
Isotype IgG
Plasmids, Primers & others

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Cellular Localization: Nucleus , Cytoplasm
Molecular Weight 54 kDa
Gene ID 1111
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: HCT116 , HCT116 cells with 30uM cisplatin treatment for 24hr

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) ICC/IF Image Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Se...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) IHC-P Image Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using C...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) WB Image Sample: 20 ug of HCT116 P53 whole cell lysate 10% SDS PAGE Chk1-phopho-S345 ...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) IHC-P Image Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Ser...