anti-Rat (Rattus) CHEK1 antibody for ELISA

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CAMK/CAMKL/CHK1 protein kinase Chk1
  • checkpoint kinase 1 S homeolog
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1.S
  • Chek1
  • chek1
Epitope
pSer317
75
59
43
36
27
18
16
15
14
13
7
6
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
527
222
190
14
14
10
10
10
7
5
5
5
4
2
1
1
Host
Rabbit
469
53
21
7
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
14
13
12
11
10
8
3
3
3
3
3
3
3
3
3
3
3
2
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
455
220
181
82
70
40
33
20
20
16
13
7
6
6
Options
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Available images

Catalog No. ABIN6255201
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.5 ABIN6255160 ELISA ICC IF IHC WB Rabbit IgG pSer280 Log in to see Polyclonal 1
19 ABIN6256251 ELISA ICC IF IHC WB Rabbit IgG pSer296 Log in to see Polyclonal 0
19 ABIN6256255 ELISA ICC IF IHC WB Rabbit IgG pSer301 Log in to see Polyclonal 0
19 ABIN6255167 ELISA ICC IF IHC WB Rabbit IgG pSer286 Log in to see Polyclonal 0
15.405727 ABIN1589998 ELISA WB Goat Internal Region Log in to see Polyclonal 0
15.405727 ABIN3181961 ELISA IHC WB Rabbit IgG pSer317 Log in to see Polyclonal 0
15.405727 ABIN3182434 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal 0
15.405727 ABIN3183903 ELISA IHC WB Rabbit IgG Ser249 Log in to see Polyclonal 0
15.405727 ABIN3183901 ELISA IF IHC WB Rabbit IgG Ser2484 Log in to see Polyclonal 0
15.405727 ABIN3183900 ELISA IF WB Rabbit IgG Ser2481 Log in to see Polyclonal 0
14.5 ABIN6260808 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 1
12.405727 ABIN3181962 ELISA IHC Rabbit IgG pSer345 Log in to see Polyclonal 0
7 ABIN1531555 IF ELISA WB Rabbit IgG AA 271-320, pSer301 Log in to see Polyclonal 2
7 ABIN1532830 IHC ELISA WB Rabbit IgG AA 286-335 Log in to see Polyclonal 0
7 ABIN6260809 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN2888962 ELISA IHC IHC (p) WB Rabbit IgG AA 286-335 Log in to see Polyclonal 0
7 ABIN6581068 IHC ELISA WB Rabbit Ser317 Log in to see Polyclonal 0
4 ABIN1532571 IF ELISA Rabbit IgG AA 271-320 Log in to see Polyclonal 1
4 ABIN1532831 ELISA WB Rabbit IgG AA 310-359 Log in to see Polyclonal 0
4 ABIN1531800 ELISA WB Rabbit IgG AA 286-335, pSer317 Log in to see Polyclonal 0

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope pSer317
(75), (59), (43), (36), (27), (18), (16), (15), (14), (13), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(527), (222), (190), (14), (14), (10), (10), (10), (7), (5), (5), (5), (4), (2), (1), (1)
Host Rabbit
(469), (53), (21), (7)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(14), (13), (12), (11), (10), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(455), (220), (181), (82), (70), (40), (33), (20), (20), (16), (13), (7), (6), (6)
Pubmed 1 reference available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN6255201) Images
Specificity Phospho-Chk1 (Ser317) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 317
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human Chk1 around the phosphorylation site of Serine 317
Isotype IgG
Plasmids, Primers & others

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 antibody (ABIN6255201) Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background

Description: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest.

Gene: CHEK1

Molecular Weight 56kDa
Gene ID 1111
UniProt O14757
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 antibody (ABIN6255201) Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:500, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 antibody (ABIN6255201) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-CHEK1 antibody (ABIN6255201)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Product cited in:

Jiang, Wang, Xing, Shen, Lian, Yi, Zhang, Yang, Liu, Zhang: "Sterigmatocystin-induced checkpoint adaptation depends on Chk1 in immortalized human gastric epithelial cells in vitro." in: Archives of toxicology, Vol. 91, Issue 1, pp. 259-270, 2017

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN6255201) back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) Western blot analysis of Chk1 phosphorylation expression in MCF7 whole cell lysates,T...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was fo...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining human vascular cancer tissue sections by IHC-P. The tis...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining human lymphoid tissue sections by IHC-P. The tissue was...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue ...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was for...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was form...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining rat spleen tissue sections by IHC-P. The tissue was for...