anti-Rat (Rattus) CHEK1 antibody for ELISA

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CAMK/CAMKL/CHK1 protein kinase Chk1
  • checkpoint kinase 1 S homeolog
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1.S
  • Chek1
  • chek1
Epitope
AA 271-320, pSer301
70
58
40
34
29
16
15
15
14
13
7
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
501
205
177
14
14
10
10
10
7
5
4
4
4
2
1
1
Host
Rabbit
441
53
22
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
14
13
12
8
8
8
3
3
3
3
3
3
3
3
3
3
3
2
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
440
210
176
79
71
40
24
22
18
16
7
7
6
6
Options
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Catalog No. ABIN1531555
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1532571 IF ELISA Rabbit IgG AA 271-320 Log in to see Polyclonal 1
1 ABIN1531801 IHC ELISA Rabbit IgG AA 310-359, pSer345 Log in to see Polyclonal 0
1 ABIN1532830 IHC ELISA WB Rabbit IgG AA 286-335 Log in to see Polyclonal 0
1 ABIN1531800 ELISA WB Rabbit IgG AA 286-335, pSer317 Log in to see Polyclonal 0
1 ABIN1532831 ELISA WB Rabbit IgG AA 310-359 Log in to see Polyclonal 0
1 ABIN3181961 ELISA IHC WB Rabbit IgG pSer317 Log in to see Polyclonal 0
1 ABIN3182434 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal 0
1 ABIN3183900 ELISA IF WB Rabbit IgG Ser2481 Log in to see Polyclonal 0
1 ABIN3183901 ELISA IF IHC WB Rabbit IgG Ser2484 Log in to see Polyclonal 0
1 ABIN3183903 ELISA IHC WB Rabbit IgG Ser249 Log in to see Polyclonal 0
1 ABIN2888805 IF ELISA Rabbit IgG AA 271-320 Log in to see Polyclonal 0
1 ABIN2888382 ELISA IHC IHC (p) Rabbit IgG pSer345 Log in to see Polyclonal 0
1 ABIN2888381 ELISA WB Rabbit IgG pSer317 Log in to see Polyclonal 0
1 ABIN2888963 ELISA WB Rabbit IgG AA 310-359 Log in to see Polyclonal 0
1 ABIN2888962 ELISA IHC IHC (p) WB Rabbit IgG AA 286-335 Log in to see Polyclonal 0
1 ABIN1589998 ELISA WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN3181962 ELISA IHC Rabbit IgG pSer345 Log in to see Polyclonal 0
1 ABIN2460510 IHC ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN601007 IHC ELISA WB Rabbit IgG pSer317 Log in to see Polyclonal 0
1 ABIN2883630 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal 0

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope AA 271-320, pSer301
(70), (58), (40), (34), (29), (16), (15), (15), (14), (13), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(501), (205), (177), (14), (14), (10), (10), (10), (7), (5), (4), (4), (4), (2), (1), (1)
Host Rabbit
(441), (53), (22), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(14), (13), (12), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(440), (210), (176), (79), (71), (40), (24), (22), (18), (16), (7), (7), (6), (6)
Pubmed 2 references available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN1531555) Images
Specificity

Chk1 (Phospho-Ser301) Antibody detects endogenous levels of Chk1 only when phosphorylated at Ser301.

PhosphorylationH:S301 M:S301 R:S301

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser301.
Isotype IgG
Plasmids, Primers & others

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background

Synonyms: CHEK1, Chk1, kinase Chk1

NCBI Gene Symbol: CHK1

Molecular Weight 54 kDa
Gene ID 1111
OMIM 603078
UniProt O14757
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Application Notes WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000
Comment

Unigene-Number: Hs.24529 (NCBI Gene Symbol: CHK1)

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-CHEK1 antibody (ABIN1531555)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Matsuoka, Ballif, Smogorzewska, McDonald, Hurov, Luo, Bakalarski, Zhao, Solimini, Lerenthal, Shiloh, Gygi, Elledge: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007

Sanchez, Wong, Thoma, Richman, Wu, Piwnica-Worms, Elledge: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 antibody (ABIN1531555) back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 271-320), (pSer301) antibody (ABIN1531555) Western blot analysis of extracts from 293 cells, using Chk1 (Phospho-Ser301) Antibod...
Immunofluorescence (IF) image for anti-Checkpoint Kinase 1 (CHEK1) (AA 271-320), (pSer301) antibody (ABIN1531555) Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The ...