We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Checkpoint Kinase 2 (CHEK2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CHEK2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-CHEK2 AntibodyTarget Details CHEK2 Application Details Handling Images
|Specificity||Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Human Chk2 (N-Terminus) Peptide|
Target Details CHEK2Product Details anti-CHEK2 Antibody Application Details Handling Images back to top
|Alternative Name||CHK2 (CHEK2 Antibody Abstract)|
|Background||The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53 (7,8). Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer (9).Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2|
|Pathways||p53 Signaling, Apoptosis, Cell Division Cycle|
Application DetailsProduct Details anti-CHEK2 Antibody Target Details CHEK2 Handling Images back to top
ELISA. Western Blot: Chk2 antibody can be used for detection of Chk2 at 0.5 to 1 μg/mL. A 60 kDaband can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Images back to top
|Buffer||PBS containing 0.02 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|