anti-Mouse (Murine) CHEK2 antibody for Immunoprecipitation

Recommended CHEK2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • cds1
  • chek2
  • chk2
  • hucds1
  • lfs2
  • pp1425
  • rad53
  • checkpoint kinase 2
  • serine/threonine-protein kinase chk2
  • checkpoint kinase 2 L homeolog
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • chek2.L
Reactivity
Human, Mouse (Murine)
503
227
204
25
18
16
13
8
5
4
3
1
1
1
1
1
Host
Rabbit
387
118
8
1
1
Clonality
Polyclonal
Conjugate
This CHEK2 antibody is un-conjugated
10
10
9
7
7
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
418
151
149
104
98
66
50
43
34
10
6
4
2
1
1
Supplier
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Available images

Catalog No. ABIN6566595
$ 438.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19 ABIN1871829 ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
13.252602 ABIN1690131 IP Magnetic Particles Rabbit Log in to see Polyclonal 0
13.252602 ABIN1690479 IP Sepharose Rabbit Log in to see Polyclonal 0
6.979721 ABIN5952373 ICC IF IHC IHC (p) IP WB Rabbit IgG AA 1-200 Log in to see SC604 0
4 ABIN2000939 IP IHC (p) WB Rabbit IgG AA 1-546 Log in to see Polyclonal 0
1 ABIN1493026 IP Magnetic Particles Rabbit C-Term Log in to see Polyclonal 0
1 ABIN1493053 IP Sepharose Rabbit C-Term Log in to see Polyclonal 0
1 ABIN210859 ICC IP WB Mouse IgG1 Log in to see 0
1 ABIN6293307 IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5703774 ELISA IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5927384 ELISA IP WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Reactivity Human, Mouse (Murine)
(503), (227), (204), (25), (18), (16), (13), (8), (5), (4), (3), (1), (1), (1), (1), (1)
Host Rabbit
(387), (118), (8), (1), (1)
Clonality
Polyclonal
Conjugate This CHEK2 antibody is un-conjugated
(10), (10), (9), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(418), (151), (149), (104), (98), (66), (50), (43), (34), (10), (6), (4), (2), (1), (1)
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Product Details anti-CHEK2 Antibody

Target Details CHEK2 Application Details Handling Images
Purification Affinity purification
Immunogen Recombinant protein of human CHEK2
Isotype IgG
Plasmids, Primers & others

Target Details CHEK2

Product Details anti-CHEK2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CHEK2 (CHEK2 Antibody Abstract)
Background

Synonyms: CDS 1,Cds1,Cds1 homolog,Checkpoint kinase 2,Checkpoint like protein CHK2,CHEK 2,Chek2,Chk 2,CHK2 checkpoint homolog (S. pombe),CHK2 checkpoint homolog,CHK2,hCds1,HuCds 1,LFS 2,LFS2,PP1425,RAD 53,RAD53,Rad53 homolog,Serine/threonine protein kinase Chk2,Serine/threonine-protein kinase Chk2

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight

Observed_MW: 60kDa

Calculated_MW: 15-38kDa/50-65kDa

Gene ID 11200
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK2 Antibody Target Details CHEK2 Handling Images back to top
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:20 - 1:50
Restrictions For Research Use only

Handling

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Images back to top
Concentration 1 mg/mL
Buffer PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze / thaw cycles.

Images

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) antibody (ABIN6566595) Western blot analysis of extracts of various cell lines, using CHEK2 antibody.
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 2 (CHEK2) antibody (ABIN6566595) Immunohistochemistry of paraffin-embedded human lung cancer using CHEK2 antibody.
Immunofluorescence (IF) image for anti-Checkpoint Kinase 2 (CHEK2) antibody (ABIN6566595) Immunofluorescence analysis of HeLa cells using CHEK2 antibody.
Immunofluorescence (IF) image for anti-Checkpoint Kinase 2 (CHEK2) antibody (ABIN6566595) Immunofluorescence analysis of A549 cells using CHEK2 antibody.
Immunoprecipitation (IP) image for anti-Checkpoint Kinase 2 (CHEK2) antibody (ABIN6566595) Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug CHEK2 antibody.