anti-Rat (Rattus) CHEK2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CHEK2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • cds1
  • chek2
  • chk2
  • hucds1
  • lfs2
  • pp1425
  • rad53
  • checkpoint kinase 2
  • serine/threonine-protein kinase chk2
  • checkpoint kinase 2 L homeolog
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • chek2.L
Epitope
AA 120-160
53
53
38
35
19
19
19
17
15
14
10
10
8
7
7
5
5
5
5
5
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
503
227
203
25
18
16
13
8
5
4
3
1
1
1
1
1
Host
Rabbit
387
118
8
1
1
Clonality
Polyclonal
Conjugate
This CHEK2 antibody is un-conjugated
10
10
9
7
7
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
418
152
149
103
99
65
50
44
34
10
6
4
2
1
1
Options
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Catalog No. ABIN685867
$ 329.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.5 ABIN703165 IF (p) IHC (p) WB Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal 2
11.424261 ABIN3043811 IHC (p) WB Rabbit IgG AA 465-498, C-Term Log in to see Polyclonal 0
11.424261 ABIN213921 ICC IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
8.424261 ABIN498829 IHC (p) Rabbit pThr387 Log in to see Polyclonal 0
8.424261 ABIN498830 IHC (p) Rabbit pThr68 Log in to see Polyclonal 0
8.424261 ABIN498917 IHC (p) Rabbit Log in to see Polyclonal 0
8.424261 ABIN498918 IHC (p) Rabbit Log in to see Polyclonal 0
7 ABIN271879 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
7 ABIN265350 IHC (p) WB Rabbit Log in to see Polyclonal 0
7 ABIN317991 IHC (p) WB Rabbit pThr68 Log in to see Polyclonal 0
4 ABIN2878986 ELISA IHC IHC (p) WB Rabbit IgG AA 35-84 Log in to see Polyclonal 0
4 ABIN5961583 ELISA IF IHC (p) WB Rabbit IgG pThr68 Log in to see Polyclonal 0
4 ABIN5961576 ELISA IHC (p) WB Rabbit IgG Thr387 Log in to see Polyclonal 0
4 ABIN5961582 ELISA IHC (p) WB Rabbit IgG pThr387 Log in to see Polyclonal 0
4 ABIN302804 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal 0
4 ABIN272011 IHC (p) WB Rabbit pSer379 Log in to see Polyclonal 0
4 ABIN1733040 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN1733039 ICC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN703174 IHC (p) WB HRP Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal 0
1 ABIN685876 IHC (p) WB HRP Rabbit IgG AA 120-160 Log in to see Polyclonal 0

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope AA 120-160
(53), (53), (38), (35), (19), (19), (19), (17), (15), (14), (10), (10), (8), (7), (7), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(503), (227), (203), (25), (18), (16), (13), (8), (5), (4), (3), (1), (1), (1), (1), (1)
Host Rabbit
(387), (118), (8), (1), (1)
Clonality
Polyclonal
Conjugate This CHEK2 antibody is un-conjugated
(10), (10), (9), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(418), (152), (149), (103), (99), (65), (50), (44), (34), (10), (6), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-CHEK2 Antibody

Target Details CHEK2 Application Details Handling References for anti-CHEK2 antibody (ABIN685867) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human CHK2
Isotype IgG
Plasmids, Primers & others

Target Details CHEK2

Product Details anti-CHEK2 Antibody Application Details Handling References for anti-CHEK2 antibody (ABIN685867) Images back to top
Antigen
Alternative Name Chk2 (CHEK2 Antibody Abstract)
Background

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.

Subcellular location: Nucleus

Synonyms: CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Checkpoint kinase 2, CHEK2

Gene ID 11200
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK2 Antibody Target Details CHEK2 Handling References for anti-CHEK2 antibody (ABIN685867) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details References for anti-CHEK2 antibody (ABIN685867) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-CHEK2 antibody (ABIN685867)

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling Images back to top
Product cited in:

Liu, Yang, Jing, Ren, Wei, Zhang, Zhang, Duan, Zhou, Sun: "Silica nanoparticle exposure inducing granulosa cell apoptosis and follicular atresia in female Balb/c mice." in: Environmental science and pollution research international, Vol. 25, Issue 4, pp. 3423-3434, 2018 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)). Further details: Sample: ovary

Wei, Zhang, Ren, Zhang, Liu, Duan, Yu, Li, Peng, Zhou, Sun: "Endosulfan induces cell dysfunction through cycle arrest resulting from DNA damage and DNA damage response signaling pathways." in: The Science of the total environment, Vol. 589, pp. 97-106, 2017 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling References for anti-CHEK2 antibody (ABIN685867) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 120-160) antibody (ABIN685867) Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti CHK2 P...
SDS-PAGE (SDS) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 120-160) antibody (ABIN685867) L1 human colon carcinoma lysates L2 human gastric carcinoma lysates probed with Anti ...