|Antigen||E2F Transcription Factor 1 (E2F1) Antibodies|
|Epitope||AA 360-369, pSer364 Alternatives|
|Reactivity||Chimpanzee, Human Alternatives|
|Conjugate||This E2F1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-E2F1 AntibodyTarget Details E2F1 Application Details Handling Images
|Specificity||This antibody is directed against the phosphorylated form of E2F-1 at the pS364 residue. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. The antibody does not cross-react with E2F-1 phosphorylated at other sites.|
Species reactivity (expected):Chimpanzee 100 % .
Species reactivity (tested):Human.
|Characteristics||Concentration: 0.80 mg/mL (by UV absorbance at 280 nm)|
|Purification||The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.|
|Immunogen||Synthetic peptide corresponding to amino acids 360-369 of Human E2F-1|
Target Details E2F1Product Details anti-E2F1 Antibody Application Details Handling Images back to top
|Alternative Name||E2F1 (E2F1 Antibody Abstract)|
|Background||E2F-1 (also known as tran-scription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several domains con-served through evolution that are found in most members of the family. These domains include a DNA binding domain, a dimerization domain that determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein as well as E2F2 and E2F3 have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner and mediates both cell proliferation and p53-dependent/independent apoptosis. Increased nuclear expression of this protein has been reported in a variety of cancers.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1|
|Pathways||p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy|
Application DetailsProduct Details anti-E2F1 Antibody Target Details E2F1 Handling Images back to top
ELISA: 1: 20,000 - 1: 100,000. Western blot: 1: 250 - 1: 2,000. Immunohistochemistry on paraffin sections: 2 μg/mL - 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-E2F1 Antibody Target Details E2F1 Application Details Images back to top
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|