anti-Human E2F1 antibody for Flow Cytometry

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Epitope
AA 69-223
44
36
31
28
22
20
17
15
14
14
12
11
11
11
10
9
9
8
8
7
6
5
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
425
124
47
10
8
6
6
5
5
4
3
3
2
1
1
Host
Mouse
288
125
21
1
Clonality
Monoclonal
Conjugate
This E2F1 antibody is un-conjugated
25
23
20
15
13
13
8
7
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
337
180
110
86
79
58
56
42
39
27
9
3
2
2
1
1
1
Options
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Catalog No. ABIN969516
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.052211 ABIN4306767 CyTOF ELISA FACS IHC IHC (p) WB Mouse IgG1 Log in to see 8G9 1
14.052211 ABIN1845545 FACS IHC ELISA WB Mouse IgG1 AA 69-223 Log in to see
1 ABIN1491636 ELISA FACS IHC (p) WB Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN4880694 ELISA FACS IHC WB Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN2256900 FACS IHC ELISA WB Mouse IgG1 Log in to see 13B231
1 ABIN2599350 ELISA FACS IF WB Rabbit IgG AA 109-138 Log in to see Polyclonal
1 ABIN5626599 ELISA FACS IHC IHC (p) WB Alexa Fluor 488 Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626600 ELISA FACS IHC IHC (p) WB Alexa Fluor 647 Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626601 ELISA FACS IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626605 ELISA FACS IHC IHC (p) WB DyLight 405 Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626598 ELISA FACS IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626609 ELISA FACS IHC IHC (p) WB DyLight 680 Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626602 FACS Allophycocyanin Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626604 ELISA FACS IHC IHC (p) WB DyLight 350 Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626607 ELISA FACS IHC IHC (p) WB DyLight 550 Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626606 ELISA FACS IHC IHC (p) WB DyLight 488 Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626608 ELISA FACS IHC IHC (p) WB DyLight 650 Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626611 ELISA FACS IHC IHC (p) FITC Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626613 FACS PerCP Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN5626614 FACS PE Mouse IgG1 AA 69-223 Log in to see 8G9

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope AA 69-223
(44), (36), (31), (28), (22), (20), (17), (15), (14), (14), (12), (11), (11), (11), (10), (9), (9), (8), (8), (7), (6), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(425), (124), (47), (10), (8), (6), (6), (5), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(288), (125), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This E2F1 antibody is un-conjugated
(25), (23), (20), (15), (13), (13), (8), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(337), (180), (110), (86), (79), (58), (56), (42), (39), (27), (9), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 antibody (ABIN969516) Images
Purification Purified
Immunogen Purified recombinant fragment of human E2F1 (AA 69-223) expressed in E. Coli.
Clone 8G9
Isotype IgG1

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 antibody (ABIN969516) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Molecular Weight 46.9 kDa
Gene ID 1869
HGNC 1869
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 antibody (ABIN969516) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 antibody (ABIN969516) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-E2F1 antibody (ABIN969516)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Product cited in:

Okazaki, Matsunaga, Okazaki, Utoguchi, Suzuki, Maruyama, Koyanagi, Ohdo: "Circadian rhythm of transferrin receptor 1 gene expression controlled by c-Myc in colon cancer-bearing mice." in: Cancer research, Vol. 70, Issue 15, pp. 6238-46, 2010

Gurtner, Fuschi, Martelli, Manni, Artuso, Simonte, Ambrosino, Antonini, Folgiero, Falcioni, Sacchi, Piaggio: "Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation." in: Cancer research, Vol. 70, Issue 23, pp. 9711-20, 2010

Locatelli, Bosotti, Ciomei, Brasca, Calogero, Mercurio, Fiorentini, Bertolotti, Scacheri, Scaburri, Galvani, Pesenti, De Baere, Soria, Lazar, Isacchi: "Transcriptional analysis of an E2F gene signature as a biomarker of activity of the cyclin-dependent kinase inhibitor PHA-793887 in tumor and skin biopsies from a phase I clinical study." in: Molecular cancer therapeutics, Vol. 9, Issue 5, pp. 1265-73, 2010

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 antibody (ABIN969516) back to top
Supplier Images
ELISA image for anti-E2F Transcription Factor 1 (E2F1) (AA 69-223) antibody (ABIN969516) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-E2F Transcription Factor 1 (E2F1) (AA 69-223) antibody (ABIN969516) Figure 2: Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and neg...
Immunohistochemistry (IHC) image for anti-E2F Transcription Factor 1 (E2F1) (AA 69-223) antibody (ABIN969516) Figure 3: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues...
Immunohistochemistry (IHC) image for anti-E2F Transcription Factor 1 (E2F1) (AA 69-223) antibody (ABIN969516) Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-E2F Transcription Factor 1 (E2F1) (AA 69-223) antibody (ABIN969516) Figure 5: Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), a...
Western Blotting (WB) image for anti-E2F Transcription Factor 1 (E2F1) (AA 69-223) antibody (ABIN969516) Figure 6: Western blot analysis using E2F1 mAb against human E2F1 recombinant protein...