anti-Human E2F1 antibody for Immunoprecipitation

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Epitope
Center
44
36
31
28
22
21
17
15
14
14
12
11
11
11
10
9
8
8
8
7
6
5
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
425
124
47
10
8
6
6
5
5
4
3
3
2
1
1
Host
Rabbit
287
126
21
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
25
23
20
15
13
13
8
7
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
337
181
111
85
79
57
55
42
39
28
9
3
2
2
1
1
1
Options
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Catalog No. ABIN2854981
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN153153 ICC IF IP IHC WB Mouse IgG1 Log in to see 18E10 2
1 ABIN269710 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see 16G7 1
1 ABIN115204 IF IHC (p) IP WB Mouse IgG2a Log in to see KH95 1
1 ABIN967439 IP WB Mouse IgG2a Log in to see KH95-E2F 5
1 ABIN153150 IP WB Mouse IgG1 Log in to see 17E2
1 ABIN210874 IP WB Mouse IgG AA 1-89, AA 342-386 Log in to see KH20-KH95
1 ABIN386823 IF IHC (p) IP WB Mouse IgG2a, kappa AA 342-386 Log in to see KH95
1 ABIN115203 IF IHC (p) IP WB Mouse IgG2a Log in to see KH95
1 ABIN153151 IP WB Mouse IgG1 Log in to see 6B5
1 ABIN153152 ICC IF IP WB Mouse IgG2b Log in to see 10D1 2B5
1 ABIN4903534 IF IP WB Mouse IgG Log in to see
1 ABIN2256896 IP WB Mouse IgG AA 1-89, AA 342-386 Log in to see KH20 and KH95
1 ABIN2256897 IF IP IHC WB Mouse IgG2a kappa AA 342-386 Log in to see 3F375
1 ABIN386825 GS IF IHC (p) IP WB Biotin Mouse IgG2a, kappa AA 342-386 Log in to see KH95
1 ABIN333021 IP WB Rabbit IgG AA 310-329 Log in to see Polyclonal
1 ABIN386827 GS IF IHC (p) IP WB Biotin Mouse IgG2a, kappa AA 342-386 Log in to see KH95
1 ABIN342248 IP WB Mouse IgG1 AA 162-476 Log in to see 1C3
1 ABIN342247 IP WB Mouse IgG1 AA 162-476 Log in to see 2F10
1 ABIN342249 IP WB Mouse IgG1 AA 162-476 Log in to see 1G10
1 ABIN189993 IP WB Mouse IgG1 AA 223-322 Log in to see

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope Center
(44), (36), (31), (28), (22), (21), (17), (15), (14), (14), (12), (11), (11), (11), (10), (9), (8), (8), (8), (7), (6), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(425), (124), (47), (10), (8), (6), (6), (5), (5), (4), (3), (3), (2), (1), (1)
Host Rabbit
(287), (126), (21), (1)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(25), (23), (20), (15), (13), (13), (8), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(337), (181), (111), (85), (79), (57), (55), (42), (39), (28), (9), (3), (2), (2), (1), (1), (1)
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling Images
Cross-Reactivity Chicken, Rat (Rattus), Cow (Bovine)
Cross-Reactivity (Details) Chicken (86 %), Rat (97 %), Bovine (96 %)
Characteristics Rabbit Polyclonal antibody to E2F1 (E2F transcription factor 1)
E2F1 antibody [N1N3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human E2F1. The exact sequence is proprietary.
Isotype IgG

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Cellular Localization: Nucleus
Molecular Weight 47 kDa
Gene ID 1869
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-E2F Transcription Factor 1 (E2F1) (Center) antibody (ABIN2854981) WB Image Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane w...
Western Blotting (WB) image for anti-E2F Transcription Factor 1 (E2F1) (Center) antibody (ABIN2854981) WB Image Sample (30 ug of whole cell lysate) A: A431 , 10% SDS PAGE antibody diluted ...
Immunoprecipitation (IP) image for anti-E2F Transcription Factor 1 (E2F1) (Center) antibody (ABIN2854981) IP Image E2F1 antibody [N1N3] immunoprecipitates E2F1 protein in IP experiments. IP s...
Immunofluorescence (IF) image for anti-E2F Transcription Factor 1 (E2F1) (Center) antibody (ABIN2854981) ICC/IF Image E2F1 antibody [N1N3] detects E2F1 protein at nucleus by immunofluorescen...