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|Antigen||E2F Transcription Factor 1 (E2F1) Antibodies|
|Epitope||AA 246-260, Middle Region Alternatives|
|Conjugate||This E2F1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|3 references available|
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Product Details anti-E2F1 AntibodyTarget Details E2F1 Application Details Handling References for anti-E2F1 antibody (ABIN3044440) Images
|Purpose||Rabbit IgG polyclonal antibody for Transcription factor E2F1(E2F1) detection. Tested with WB in Human.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Transcription factor E2F1(E2F1) detection. Tested with WB in Human.
Gene Name: E2F transcription factor 1
Protein Name: Transcription factor E2F1(E2F-1)
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human E2F1(246-260aa VTCQDLRSIADPAEQ), identical to the related mouse and rat sequences.|
Target Details E2F1Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 antibody (ABIN3044440) Images back to top
|Alternative Name||E2F1 (E2F1 Antibody Abstract)|
Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family of transcription factors appears to play a critical role in the transcription of certain genes required for cell cycle progression. E2F1, the first cloned member of this family, is regulated during the cell cycle at the mRNA level by changes in transcription of the E2F1 gene and at the protein level by complex formation with proteins such as the retinoblastoma gene product(pRB), cyclin A and DP1. E2F1 can override a pRB-induced G1/S block and can behave as an oncogene in certain cells. E2F1 was cloned and was found to contain seven exons. Fluorescence in situ hybridization localized E2F1 to chromosome 20q11. The E2F1 transcription factor can promote proliferation or apoptosis when activated, and is a key downstream target of the retinoblastoma tumour suppressor protein(pRB).
Synonyms: Dmel\CG6376 antibody|Dmel_CG6376 antibody|drosE2F1 antibody|E(Sev-CycE)3A antibody|E(var)3-93E antibody|E2-promoter binding facto antibody|E2F 1 antibody|E2F transcription factor 1 antibody|E2F-1 antibody|E2f-PA antibody|E2f-PB antibody|E2f-PC antibody|E2F1 antibody|E2f1 E2F transcription factor 1 antibody|E2F1_HUMAN antibody|Evar(3)164 antibody|KIAA4009 antibody|l(3)07172 antibody|l(3)j3B1 antibody|l(3)j3C2 antibody|l(3)rM729 antibody|mKIAA4009 antibody|OTTHUMP00000030661 antibody|PBR 3 antibody|PBR3 antibody|PRB binding protein E2F 1 antibody|PRB-binding protein E2F-1 antibody|RBAP 1 antibody|RBAP-1 antibody|RBAP1 antibody|RBBP 3 antibody|RBBP-3 antibody|RBBP3 antibody|RBP 3 antibody|RBP3 antibody|Retinoblastoma associated protein 1 antibody|Retinoblastoma binding protein 3 antibody|Retinoblastoma-associated protein 1 antibody|Retinoblastoma-binding protein 3 antibody|Transcription factor E2F1 antibody
|Pathways||p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy|
Application DetailsProduct Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 antibody (ABIN3044440) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
|Restrictions||For Research Use only|
HandlingProduct Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 antibody (ABIN3044440) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
References for anti-E2F1 antibody (ABIN3044440)Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
|Product cited in:||
Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." in: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, 2015
Xie, Li, Wang, Jiao, Zhang, Zhang: "miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells." in: Oncology reports, Vol. 23, Issue 6, pp. 1693-700, 2010
Wang, Li, Zhang, Li, Yue, Xie, Xie: "Regulating A549 cells growth by ASO inhibiting miRNA expression." in: Molecular and cellular biochemistry, Vol. 339, Issue 1-2, pp. 163-71, 2010
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