anti-Human E2F1 antibody for Western Blotting

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor 1
  • E2F transcription factor 1 L homeolog
  • E2f1
  • E2F1
  • e2f1.L
Epitope
AA 246-260, Middle Region
44
36
33
29
22
21
17
15
14
14
12
11
11
11
10
9
9
8
8
7
6
5
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
425
126
46
11
8
6
6
5
5
4
3
3
2
1
1
Host
Rabbit
288
124
23
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
25
23
20
15
15
13
8
7
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
Application
Western Blotting (WB)
342
190
114
87
80
56
55
42
41
29
9
3
2
2
2
1
1
Options
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Catalog No. ABIN3044440
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2286417 ABIN2792603 WB Rabbit N-Term Log in to see Polyclonal 1
3.2286417 ABIN2792180 WB Rabbit C-Term Log in to see Polyclonal 1
3.2286417 ABIN391858 WB Rabbit Ig Fraction AA 342-371, His357 Log in to see Polyclonal 6
3.2286417 ABIN2854981 ICC IF IP WB Rabbit IgG Center Log in to see Polyclonal 0
3.2286417 ABIN2704740 WB Rabbit C-Term, pSer433 Log in to see Polyclonal 0
3.2286417 ABIN2704741 IHC WB Rabbit C-Term Log in to see Polyclonal 0
3.2286417 ABIN391857 WB Rabbit Ig Fraction AA 310-344, Ser332 Log in to see Polyclonal 5
3.2286417 ABIN389553 IHC (p) WB Rabbit Ig Fraction pSer337 Log in to see Polyclonal 5
3.2286417 ABIN969516 FACS IHC ELISA WB Mouse IgG1 AA 69-223 Log in to see 8G9 3
3.2286417 ABIN4306767 CyTOF ELISA FACS IHC IHC (p) WB Mouse IgG1 Log in to see 8G9 1
3.2286417 ABIN359769 EIA WB Rabbit Ig Fraction His357 Log in to see Polyclonal 5
3.2286417 ABIN359768 EIA WB Rabbit Ig Fraction Ser332 Log in to see Polyclonal 5
3.2286417 ABIN441399 ICC IF WB Rabbit IgG Center Log in to see Polyclonal 0
3.2286417 ABIN4306768 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
3.2286417 ABIN2777217 WB Rabbit Middle Region Log in to see Polyclonal 0
3.2286417 ABIN1531270 ELISA WB Rabbit IgG AA 388-437, pThr433 Log in to see Polyclonal 0
3.2286417 ABIN1532290 ELISA WB Rabbit IgG AA 388-437 Log in to see Polyclonal 0
3.2286417 ABIN269710 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see 16G7 1
3.2286417 ABIN129619 IHC ELISA WB Rabbit IgG AA 360-369, pSer364 Log in to see Polyclonal 0
3.2286417 ABIN967439 IP WB Mouse IgG2a Log in to see KH95-E2F 5

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope AA 246-260, Middle Region
(44), (36), (33), (29), (22), (21), (17), (15), (14), (14), (12), (11), (11), (11), (10), (9), (9), (8), (8), (7), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(425), (126), (46), (11), (8), (6), (6), (5), (5), (4), (3), (3), (2), (1), (1)
Host Rabbit
(288), (124), (23), (1)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(25), (23), (20), (15), (15), (13), (8), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Western Blotting (WB)
(342), (190), (114), (87), (80), (56), (55), (42), (41), (29), (9), (3), (2), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 antibody (ABIN3044440) Images
Purpose Rabbit IgG polyclonal antibody for Transcription factor E2F1(E2F1) detection. Tested with WB in Human.
Sequence VTCQDLRSIA DPAEQ
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Transcription factor E2F1(E2F1) detection. Tested with WB in Human.
Gene Name: E2F transcription factor 1
Protein Name: Transcription factor E2F1(E2F-1)
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human E2F1(246-260aa VTCQDLRSIADPAEQ), identical to the related mouse and rat sequences.
Isotype IgG

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 antibody (ABIN3044440) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family of transcription factors appears to play a critical role in the transcription of certain genes required for cell cycle progression. E2F1, the first cloned member of this family, is regulated during the cell cycle at the mRNA level by changes in transcription of the E2F1 gene and at the protein level by complex formation with proteins such as the retinoblastoma gene product(pRB), cyclin A and DP1. E2F1 can override a pRB-induced G1/S block and can behave as an oncogene in certain cells. E2F1 was cloned and was found to contain seven exons. Fluorescence in situ hybridization localized E2F1 to chromosome 20q11. The E2F1 transcription factor can promote proliferation or apoptosis when activated, and is a key downstream target of the retinoblastoma tumour suppressor protein(pRB).

Synonyms: Dmel\CG6376 antibody|Dmel_CG6376 antibody|drosE2F1 antibody|E(Sev-CycE)3A antibody|E(var)3-93E antibody|E2-promoter binding facto antibody|E2F 1 antibody|E2F transcription factor 1 antibody|E2F-1 antibody|E2f-PA antibody|E2f-PB antibody|E2f-PC antibody|E2F1 antibody|E2f1 E2F transcription factor 1 antibody|E2F1_HUMAN antibody|Evar(3)164 antibody|KIAA4009 antibody|l(3)07172 antibody|l(3)j3B1 antibody|l(3)j3C2 antibody|l(3)rM729 antibody|mKIAA4009 antibody|OTTHUMP00000030661 antibody|PBR 3 antibody|PBR3 antibody|PRB binding protein E2F 1 antibody|PRB-binding protein E2F-1 antibody|RBAP 1 antibody|RBAP-1 antibody|RBAP1 antibody|RBBP 3 antibody|RBBP-3 antibody|RBBP3 antibody|RBP 3 antibody|RBP3 antibody|Retinoblastoma associated protein 1 antibody|Retinoblastoma binding protein 3 antibody|Retinoblastoma-associated protein 1 antibody|Retinoblastoma-binding protein 3 antibody|Transcription factor E2F1 antibody
UniProt Q01094
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 antibody (ABIN3044440) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 antibody (ABIN3044440) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-E2F1 antibody (ABIN3044440)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Product cited in:

Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." in: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, 2015

Xie, Li, Wang, Jiao, Zhang, Zhang: "miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells." in: Oncology reports, Vol. 23, Issue 6, pp. 1693-700, 2010

Wang, Li, Zhang, Li, Yue, Xie, Xie: "Regulating A549 cells growth by ASO inhibiting miRNA expression." in: Molecular and cellular biochemistry, Vol. 339, Issue 1-2, pp. 163-71, 2010

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 antibody (ABIN3044440) back to top
Supplier Images
Western Blotting (WB) image for anti-E2F Transcription Factor 1 (E2F1) (AA 246-260), (Middle Region) antibody (ABIN3044440) Observed bind size: 60KD