anti-Mouse (Murine) E2F1 antibody for Immunohistochemistry (Frozen Sections)

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor 1
  • E2F transcription factor 1 L homeolog
  • E2f1
  • E2F1
  • e2f1.L
Epitope
acLys120, acLys125
35
35
33
30
25
20
19
19
17
16
15
12
12
11
11
10
10
9
8
7
7
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
441
133
52
12
8
6
6
5
5
4
3
3
2
1
1
Host
Rabbit
300
128
25
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
25
24
21
15
15
13
8
7
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
355
203
134
93
81
63
56
42
41
29
8
3
2
1
1
1
1
Supplier
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Catalog No. ABIN1107042
$ 555.50
Plus shipping costs $45.00

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope acLys120, acLys125
(35), (35), (33), (30), (25), (20), (19), (19), (17), (16), (15), (12), (12), (11), (11), (10), (10), (9), (8), (7), (7), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(441), (133), (52), (12), (8), (6), (6), (5), (5), (4), (3), (3), (2), (1), (1)
Host Rabbit
(300), (128), (25)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(25), (24), (21), (15), (15), (13), (8), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(355), (203), (134), (93), (81), (63), (56), (42), (41), (29), (8), (3), (2), (1), (1), (1), (1)
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling Images
Specificity Reacts with Human 47 kDa E2F-1 acK120 and acK125.
Cross-Reactivity (Details) Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein A
Immunogen Synthetic peptide derived from internal domain of Human E2F-1 protein containing acetyl lysine 120 and 125
Plasmids, Primers & others

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background E2F-1 (also known as transcription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several domains con-served through evolution that are found in most members of the family. These domains include a DNA binding domain, a dimerization domain that determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein as well as E2F2 and E2F3 have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner and mediates both cell proliferation and p53-dependent/independent apoptosis. Increased nuclear expression of this protein has been reported in a variety of cancers.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1
Gene ID 1869
NCBI Accession NP_005216
UniProt Q01094
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Images back to top
Reconstitution Restore in distilled water.
Concentration 1.0mg/mL
Buffer 0.1 M Tris, 0.1 M Glycine, 2 % Sucrose, None
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store the antibody at-20 °C. Store reconstituted antibody at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling back to top
Supplier Images
 image for anti-E2F Transcription Factor 1 (E2F1) (acLys120), (acLys125) antibody (ABIN1107042) anti-E2F Transcription Factor 1 (E2F1) (acLys120), (acLys125) antibody