We are preparing the requested document. Please wait, this may take a while...!
|Antigen||E2F Transcription Factor 1 (E2F1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This E2F1 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-E2F1 AntibodyTarget Details E2F1 Application Details Handling References for anti-E2F1 antibody (ABIN967439) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
Target Details E2F1Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 antibody (ABIN967439) Images back to top
|Alternative Name||E2F-1 (E2F1 Antibody Abstract)|
|Background||The transcription factor E2F was originally characterized as a sequence-specific DNA-binding factor bound to the adenovirus E2A promoter. E2F-binding sites have now been identified in cellular promoters for genes involved in growth regulation, including c-myc, N-myc, Cdc2 and cyclin A. They have also been identified in promoters for genes whose products are required for DNA synthesis and replication, such as DNA polymerase a, thymidine kinase, thymidylate synthase, and dihydrofolate reductase. When bound to DNA, E2F has been found in complexes with key regulators of cell proliferation including the retinoblastoma protein (Rb) and the Rb related proteins, p107 and p130. Like a variety of other transcription factors, E2F binds to DNA as a heterodimer. The protein E2F-1 has E2F-like properties, it mediates E2F-dependent trans-activation and binds to underphosphorylated Rb. E2F dimerizes with DP-1, a protein which also has E2F-like properties. This dimerization leads to increased DNA binding activity and enhanced transcriptional activity. It is thought that E2F-1/DP association forms an active E2F” transcription factor. E2F-1 migrates at a reduced molecular weight of ~60 kDa. Clone KH95/E2F (also referred to as KH95) recognizes an epitope between amino acids 342 and 386 of human E2F-1. It cross reacts with mouse and rat E2F-1. Full length, bacterially expressed, recombinant histidine-tagged human E2F-1 (amino acids 1-437) was purified and used as immunogen. Positive tissue culture supernatants were identified by immunoprecipitation of [35S]methionine-labeled, in vitro synthesized E2F-1 protein.|
|Molecular Weight||60 kDa|
|Pathways||p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy|
Application DetailsProduct Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 antibody (ABIN967439) Images back to top
|Application Notes||Applications include western blot analysis (dilute antibody 1-2 µg/ml). MOLT-4 leukemia cells (ATCC CRL-1582), Daudi lymphoma cells (ATCC CCL-213), and RS4, 11 leukemia cells (ATCC CRL-1873) are suggested as positive controls. Other applications include immunoprecipitation (1-2 µg/1x10^6 cells).|
|Restrictions||For Research Use only|
HandlingProduct Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 antibody (ABIN967439) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-E2F1 antibody (ABIN967439)Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
|Product cited in:||
Saito, Helin, Valentine, Griffith, Willman, Harlow, Look: "Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line." in: Genomics, Vol. 25, Issue 1, pp. 130-8, 1995
Dyson, Dembski, Fattaey, Ngwu, Ewen, Helin: "Analysis of p107-associated proteins: p107 associates with a form of E2F that differs from pRB-associated E2F-1." in: Journal of virology, Vol. 67, Issue 12, pp. 7641-7, 1993
Helin, Wu, Fattaey, Lees, Dynlacht, Ngwu, Harlow: "Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation." in: Genes & development, Vol. 7, Issue 10, pp. 1850-61, 1993
Huber, Edwards, Goodhart, Patrick, Huang, Ivey-Hoyle, Barnett, Oliff, Heimbrook: "Transcription factor E2F binds DNA as a heterodimer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3525-9, 1993
Nevins: "E2F: a link between the Rb tumor suppressor protein and viral oncoproteins." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 424-9, 1992
ImagesProduct Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 antibody (ABIN967439) back to top