anti-Xenopus laevis E2F1 antibody for Immunoprecipitation

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor 1
  • E2F transcription factor 1 L homeolog
  • E2f1
  • E2F1
  • e2f1.L
Epitope
AA 1-89, AA 342-386
35
35
33
30
25
20
19
19
17
16
15
12
12
11
11
10
10
9
8
7
7
6
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Xenopus laevis
441
134
52
12
8
6
6
5
5
4
3
3
2
1
Host
Mouse
301
127
25
Clonality
Monoclonal
Conjugate
This E2F1 antibody is un-conjugated
25
24
21
15
15
13
8
7
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
355
203
134
93
81
63
55
42
41
29
9
3
2
2
1
1
1
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Catalog No. ABIN210874
$ 903.83
Plus shipping costs $45.00

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope AA 1-89, AA 342-386
(35), (35), (33), (30), (25), (20), (19), (19), (17), (16), (15), (12), (12), (11), (11), (10), (10), (9), (8), (7), (7), (6), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Xenopus laevis
(441), (134), (52), (12), (8), (6), (6), (5), (5), (4), (3), (3), (2), (1)
Host Mouse
(301), (127), (25)
Clonality (Clone)
Monoclonal   ( )
Conjugate This E2F1 antibody is un-conjugated
(25), (24), (21), (15), (15), (13), (8), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunoprecipitation (IP), Western Blotting (WB)
(355), (203), (134), (93), (81), (63), (55), (42), (41), (29), (9), (3), (2), (2), (1), (1), (1)
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling Images
Specificity Recognizes human E2F-1 at 55kD. Species cross-reactivity: Xenopus.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%) Rat, Elephant (94%) Opossum (89%) Chicken, Lizard (83%).
Purification Protein G purified
Immunogen GST fusion protein corresponding to full-length human E2F1 (Accession #NP_005216). Epitope: aa1-89 for clone KH20, aa342-386 for clone KH95.

Type of Immunogen: Fusion protein
Clone KH20-KH95
Isotype IgG
Plasmids, Primers & others

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background Name/Gene ID: E2F1

Synonyms: E2F1, E2F transcription factor 1, PRB-binding protein E2F-1, RBAP-1, RBBP3, RBBP-3, Transcription factor E2F1, RBAP1, E2F-1, PBR3
Gene ID 1869
NCBI Accession NP_005216
UniProt Q01094
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling Images back to top
Application Notes Approved: IP, WB

Usage: Western Blot Analysis: 0.5-2 μg/mL of antibody detected E2F-1 (~55kD) in a HeLa nuclear extract. Positive Antigen Control: HeLa nuclear extract. Use 20 μg per lane. Immunoprecipitation: 4 μg of a previous lot of antibody immunoprecipitated E2F-1 from HeLa nuclear extract.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.