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General |
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Antigen | Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies |
Epitope | Extracellular Domain Alternatives |
Reactivity | Cat (Feline) Alternatives |
Host | Mouse Alternatives |
Clonality | |
Conjugate | This FAS antibody is conjugated to PE Alternatives |
Application |
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-FAS AntibodyTarget Details FAS Application Details Handling Images |
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Specificity | Recognizes feline Fas extracellular domain. Species sequence homology: human 68 % , mouse 65 %, porcine 53 %, and rat 58 %. Does not cross-react with rhFas, rmFas, or rrFas. |
Purification | Protein G purified |
Immunogen |
NS0-derived recombinant feline rfeFas extracellular domain. Type of Immunogen: Recombinant protein |
Isotype | IgG1 |
Plasmids, Primers & others | |
Target Details FASProduct Details anti-FAS Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | CD95 / FAS (FAS Antibody Abstract) |
Background |
Name/Gene ID: FAS Family: TNF Receptor Synonyms: FAS, ALPS1A, APO-1, APT1, Apo-1 antigen, APO-1 cell surface antigen, Apo11 antigen, Apoptosis antigen 1, CD95 antigen, Delta Fas/APO-1/CD95, Fas AMA, FAS1, Fasl receptors, FASLG receptor, FASTM, FAS 827dupA, Fasl receptor, TNFRSF6, CD95 |
Gene ID | 355 |
UniProt | P25445 |
Pathways | p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity |
Application DetailsProduct Details anti-FAS Antibody Target Details FAS Handling Images back to top |
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Application Notes |
Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (1 - 2 μg/mL) Usage: Suitable for use in Flow cytometry, Western blot and Direct ELISA. Flow cytometry: 25 μg/mL and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: 1-2 μg/mL. the detection limit for rfeFas is approximately 25 ng/lane under non-reducing conditions. It is not recommended to use this antibody under reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 μg/mL The detection limit for rfeFas is approximately 3 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. |
Comment |
Target Species of Antibody: Cat |
Restrictions | For Research Use only |
HandlingProduct Details anti-FAS Antibody Target Details FAS Application Details Images back to top |
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Format | Lyophilized |
Reconstitution | Reconstitute, sterile PBS to a concentration of 0.5 mg/mL. |
Concentration | Lot specific |
Buffer | Lyophilized powder from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL. |