anti-Cat (Feline) FAS antibody for ELISA

Recommended FAS Antibody (supplied by: Log in to see )

Antigen
Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
  • ALPS1A
  • APO-1
  • APT1
  • CD95
  • FAS1
  • FASTM
  • TNFRSF6
  • AI196731
  • APO1
  • TNFR6
  • Tnfrsf6
  • lpr
  • Fas cell surface death receptor
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
  • Fas (TNF receptor superfamily member 6)
  • FAS
  • YWHAZ
  • Fas
  • fas
Epitope
Extracellular Domain
48
25
18
15
15
15
13
12
12
12
11
9
9
8
8
7
7
6
5
5
5
5
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline)
660
114
96
10
7
5
4
3
3
3
2
2
1
Host
Mouse
416
313
24
5
4
3
3
Clonality
Monoclonal
Conjugate
This FAS antibody is conjugated to PE
70
59
46
29
16
9
7
6
5
5
5
4
4
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
339
337
208
169
89
76
75
73
52
41
20
19
11
8
7
7
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN323507
$ 925.83
Plus shipping costs $45.00

General

Antigen Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
Epitope Extracellular Domain
(48), (25), (18), (15), (15), (15), (13), (12), (12), (12), (11), (9), (9), (8), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline)
(660), (114), (96), (10), (7), (5), (4), (3), (3), (3), (2), (2), (1)
Host Mouse
(416), (313), (24), (5), (4), (3), (3)
Clonality
Monoclonal
Conjugate This FAS antibody is conjugated to PE
(70), (59), (46), (29), (16), (9), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(339), (337), (208), (169), (89), (76), (75), (73), (52), (41), (20), (19), (11), (8), (7), (7), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-FAS Antibody

Target Details FAS Application Details Handling Images
Specificity Recognizes feline Fas extracellular domain. Species sequence homology: human 68 % , mouse 65 %, porcine 53 %, and rat 58 %. Does not cross-react with rhFas, rmFas, or rrFas.
Purification Protein G purified
Immunogen NS0-derived recombinant feline rfeFas extracellular domain.

Type of Immunogen: Recombinant protein
Isotype IgG1
Plasmids, Primers & others

Target Details FAS

Product Details anti-FAS Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD95 / FAS (FAS Antibody Abstract)
Background Name/Gene ID: FAS
Family: TNF Receptor

Synonyms: FAS, ALPS1A, APO-1, APT1, Apo-1 antigen, APO-1 cell surface antigen, Apo11 antigen, Apoptosis antigen 1, CD95 antigen, Delta Fas/APO-1/CD95, Fas AMA, FAS1, Fasl receptors, FASLG receptor, FASTM, FAS 827dupA, Fasl receptor, TNFRSF6, CD95
Gene ID 355
UniProt P25445
Pathways p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FAS Antibody Target Details FAS Handling Images back to top
Application Notes Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow cytometry, Western blot and Direct ELISA. Flow cytometry: 25 μg/mL and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: 1-2 μg/mL. the detection limit for rfeFas is approximately 25 ng/lane under non-reducing conditions. It is not recommended to use this antibody under reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 μg/mL The detection limit for rfeFas is approximately 3 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Comment

Target Species of Antibody: Cat
Restrictions For Research Use only

Handling

Product Details anti-FAS Antibody Target Details FAS Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
Concentration Lot specific
Buffer Lyophilized powder from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.