anti-Mouse (Murine) FAS antibody for Cytotoxicity Test

Recommended FAS Antibody (supplied by: Log in to see )

Antigen
Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
  • ALPS1A
  • APO-1
  • APT1
  • CD95
  • FAS1
  • FASTM
  • TNFRSF6
  • AI196731
  • APO1
  • TNFR6
  • Tnfrsf6
  • lpr
  • Fas cell surface death receptor
  • Fas (TNF receptor superfamily member 6)
  • FAS
  • Fas
  • fas
Reactivity
Mouse (Murine)
638
112
99
13
11
10
6
6
3
3
3
3
2
1
Host
Armenian Hamster
408
300
24
5
4
3
2
Clonality
Monoclonal
Conjugate
This FAS antibody is un-conjugated
73
63
49
30
15
8
7
6
5
5
5
4
4
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
Application
Cytotoxicity Test (CyTox), Flow Cytometry (FACS), Immunoprecipitation (IP)
332
321
210
146
105
82
61
54
52
47
23
19
12
7
7
6
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN2689461
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177304 CyTox FACS IP Armenian Hamster IgG2, lambda Log in to see Jo2 9

General

Antigen Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
Reactivity Mouse (Murine)
(638), (112), (99), (13), (11), (10), (6), (6), (3), (3), (3), (3), (2), (1)
Host Armenian Hamster
(408), (300), (24), (5), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FAS antibody is un-conjugated
(73), (63), (49), (30), (15), (8), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Cytotoxicity Test (CyTox), Flow Cytometry (FACS), Immunoprecipitation (IP)
(332), (321), (210), (146), (105), (82), (61), (54), (52), (47), (23), (19), (12), (7), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-FAS Antibody

Target Details FAS Application Details Handling References for anti-FAS antibody (ABIN2689461) Images
Brand BD Pharmingen™
Characteristics Fas antigen, CD95, is a 45 kDa cell-surface protein which can mediate apoptosis. It belongs to the TNF (tumor necrosis factor)/NGF receptor family. Expression of Fas has been described in the thymus, liver, heart, lung and ovary. Fas plays an important role in the apoptotic process that takes place during development. Monoclonal antibodies recognizing Fas such as Jo2 have cytolytic activity on cells expressing Fas. The cell death stimulated by Fas antibodies is characteristic of apoptosis and suggests that the lethal effects are a result of interaction of antibody with a functional Fas antigen as opposed to complement-mediated lysis. The Jo2 antibody recognizes mouse Fas. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis. Intraperitoneal injections of Jo2 mAb have been shown to kill mice and induce apoptotic hepatocyte death. Jo2 mAb immunoprecipitates mouse Fas as a 45 kDa band from W4 cells. W4 cells are WR19L mouse lymphoma cells transformed with mouse Fas. The difference between the observed MW of Fas and that deduced from its amino acid sequence (Mr 34,971) may be due to glycosylation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of Fas on mouse thymocytes analyzed by flow cytometry. Thymocytes from a C57BL/6 mouse were incubated with either Jo2, followed by a FITC-conjugated mouse anti-hamster second step (filled histogram, or with second step alone (open histogram).

BD Pharmingen™ Purified Hamster Anti-Mouse CD95 - Purified - Clone Jo2 - Isotype Armenian Hamster IgG2, λ2 - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen WR19L mouse lymphoma cells transformed with recombinant mouse Fas
Clone Jo2
Isotype IgG2, lambda

Target Details FAS

Product Details anti-FAS Antibody Application Details Handling References for anti-FAS antibody (ABIN2689461) Images back to top
Antigen
Alternative Name CD95 (FAS Antibody Abstract)
Background Synonyms: Fas/APO-1
Molecular Weight 45 kDaTarget Molecular Weight: Aqueous buffered solution containing ≤0.09% sodium azide.
Pathways p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FAS Antibody Target Details FAS Handling References for anti-FAS antibody (ABIN2689461) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-FAS Antibody Target Details FAS Application Details References for anti-FAS antibody (ABIN2689461) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-FAS antibody (ABIN2689461)

Product Details anti-FAS Antibody Target Details FAS Application Details Handling Images back to top
Product cited in:

Ogasawara, Suda, Nagata: "Selective apoptosis of CD4+CD8+ thymocytes by the anti-Fas antibody." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 485-91, 1995

Yang, Merćep, Ware, Ashwell: "Fas and activation-induced Fas ligand mediate apoptosis of T cell hybridomas: inhibition of Fas ligand expression by retinoic acid and glucocorticoids." in: The Journal of experimental medicine, Vol. 181, Issue 5, pp. 1673-82, 1995

Hiromatsu, Aoki, Makino, Matsumoto, Mizuochi, Gotoh, Nomoto, Ogasawara, Nagata, Yoshikai: "Increased Fas antigen expression in murine retrovirus-induced immunodeficiency syndrome, MAIDS." in: European journal of immunology, Vol. 24, Issue 10, pp. 2446-51, 1994

Kägi, Vignaux, Ledermann, Bürki, Depraetere, Nagata, Hengartner, Golstein: "Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity." in: Science (New York, N.Y.), Vol. 265, Issue 5171, pp. 528-30, 1994

Ogasawara, Watanabe-Fukunaga, Adachi, Matsuzawa, Kasugai, Kitamura, Itoh, Suda, Nagata: "Lethal effect of the anti-Fas antibody in mice." in: Nature, Vol. 364, Issue 6440, pp. 806-9, 1993