anti-Mouse (Murine) FAS antibody for Functional Studies

Recommended FAS Antibody (supplied by: Log in to see )

Antigen
Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
  • ALPS1A
  • APO-1
  • APT1
  • CD95
  • FAS1
  • FASTM
  • TNFRSF6
  • AI196731
  • APO1
  • TNFR6
  • Tnfrsf6
  • lpr
  • Fas cell surface death receptor
  • Fas (TNF receptor superfamily member 6)
  • FAS
  • Fas
  • fas
Reactivity
Human, Mouse (Murine), Rat (Rattus)
636
112
98
13
11
10
6
6
3
3
3
3
2
1
Host
Mouse
406
300
24
5
4
3
3
Clonality
Monoclonal
Conjugate
This FAS antibody is un-conjugated
73
63
49
30
15
8
7
6
5
5
5
4
4
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
332
319
210
146
104
82
60
54
52
46
23
19
11
7
6
6
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
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Catalog No. ABIN187300
$ 451.00
Plus shipping costs $45.00

General

Antigen Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(636), (112), (98), (13), (11), (10), (6), (6), (3), (3), (3), (3), (2), (1)
Host Mouse
(406), (300), (24), (5), (4), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FAS antibody is un-conjugated
(73), (63), (49), (30), (15), (8), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(332), (319), (210), (146), (104), (82), (60), (54), (52), (46), (23), (19), (11), (7), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-FAS Antibody

Target Details FAS Application Details Handling Images
Specificity Reacts with human Fas antigen. Species Reactivity: Reacts with human Fas antigen. Reacts with mouse and rat Fas in Western blot.
Immunogen Recombinant human Fas
Clone 5F-9
Isotype IgG1

Target Details FAS

Product Details anti-FAS Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD95 / FAS (FAS Antibody Abstract)
Background The cell-surface Fas antigen is a 48 kDa transmembrane protein that can mediate apoptosis/programmed cell death and belongs to the nerve growth factor (NGF)/tumor necrosis factor (TNF) receptor superfamily. The human Fas antigen is expressed in various human cells, including activated peripheral T and B lymphocytes, various lymphoid tumor cell lines, some leukemic T cells, and some myeloid cells. It is also differentially expressed on human thymocytes during thymic maturation.
Pathways p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FAS Antibody Target Details FAS Handling Images back to top
Application Notes Functional activity: Prevents apoptosis induced by actinomycin D and cycloheximide, but not Fas antigen. Use at 0.5-5 µg/mL on 2 x 104 cells grown in DMEM medium with 10% FCS in a microtiter well. ELISA: Use at a 1:1,000 dilution. Immunoprecipitation: Use at 2-5 µg/106 cells. Immunohistochemistry: reportedly works for some tissues (frozen) at a 1:50-1:100 dilution (10-20 µg/mL). Recommended diluent is PBS with 2% FCS and 0.01% sodium azide. Western blot: Use at 2-5 µg/mL. The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only

Handling

Product Details anti-FAS Antibody Target Details FAS Application Details Images back to top
Format Liquid
Buffer 100 µL of ~1 mg/mL (100 µg) monoclonal antibody in 1X PBS, pH 7.2, with 0.01% sodium azide. (See vial label for lot specific volume and concentration.) Purified by Protein A chromatography.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C