anti-Bat HMGB1 antibody for Western Blotting

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • amphoterin
  • p30
  • hmgb1
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • hmg-1
  • hmg3
  • sbp-1
  • hmg1
  • HMGB1
  • Ac2-008
  • high mobility group box 1
  • high-mobility group box 1
  • high mobility group box 1a
  • high mobility group box 1 L homeolog
  • high mobility group protein B1
  • HMGB1
  • Hmgb1
  • hmgb1
  • hmgb1a
  • hmgb1.L
  • LOC100359149
Epitope
AA 126-175
66
60
38
38
33
16
16
16
15
15
15
14
14
11
11
11
7
6
5
5
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
443
237
167
54
38
34
23
14
12
10
10
9
6
4
3
2
1
Host
Rabbit
262
197
3
2
2
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
29
24
17
15
13
8
8
7
7
7
6
6
6
6
5
5
5
4
3
2
2
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
396
186
161
131
116
94
47
26
21
13
12
8
6
3
2
2
1
1
1
1
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Catalog No. ABIN324443
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN203033 WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN575653 WB Rabbit AA 21-70 Log in to see Polyclonal 0
1 ABIN2621941 IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2621942 IHC IHC (p) WB Rabbit AA 121-170 Log in to see Polyclonal 0

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope AA 126-175
(66), (60), (38), (38), (33), (16), (16), (16), (15), (15), (15), (14), (14), (11), (11), (11), (7), (6), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(443), (237), (167), (54), (38), (34), (23), (14), (12), (10), (10), (9), (6), (4), (3), (2), (1)
Host Rabbit
(262), (197), (3), (2), (2), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(29), (24), (17), (15), (13), (8), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(396), (186), (161), (131), (116), (94), (47), (26), (21), (13), (12), (8), (6), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling Images
Specificity Human HMGB1
Predicted Reactivity Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Xenopus, Zebrafish (92%).
Purification Protein A purified
Immunogen Synthetic peptide located between aa126-175 of human HMGB1 (P09429, NP_002119). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Smelt (100%), Xenopus, Frog, Zebrafish (92%), Trout, Salmon, Sablefish (85%).

Type of Immunogen: Synthetic peptide
Isotype IgG
Plasmids, Primers & others

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HMG1 / HMGB1 (HMGB1 Antibody Abstract)
Background Name/Gene ID: HMGB1

Synonyms: HMGB1, Amphoterin, High mobility group protein B1, High mobility group box 1, High mobility group protein 1, HMG1, HMG3, SBP-1, High-mobility group box 1, HMG-1
Gene ID 3146
NCBI Accession NP_002119
UniProt P09429
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top
Application Notes Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top
Format Lyophilized
Reconstitution Distilled Water.
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top
Supplier Images
 image for anti-High-Mobility Group Box 1 (HMGB1) (AA 126-175) antibody (ABIN324443) anti-High-Mobility Group Box 1 (HMGB1) (AA 126-175) antibody