anti-Human HMGB1 antibody for Immunohistochemistry (Frozen Sections)

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • amphoterin
  • p30
  • hmgb1
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • hmg-1
  • hmg3
  • sbp-1
  • hmg1
  • HMGB1
  • Ac2-008
  • high mobility group box 1
  • high-mobility group box 1
  • high mobility group box 1a
  • high mobility group box 1 L homeolog
  • high mobility group protein B1
  • HMGB1
  • Hmgb1
  • hmgb1
  • hmgb1a
  • hmgb1.L
  • LOC100359149
Reactivity
Human
463
240
168
55
41
35
24
14
13
11
11
10
7
5
3
2
1
Host
Mouse
265
215
3
2
2
2
Clonality
Monoclonal
Conjugate
This HMGB1 antibody is un-conjugated
29
24
17
17
15
9
8
8
8
7
6
6
6
6
6
5
5
5
4
3
3
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
411
189
167
137
118
89
43
27
26
13
12
8
6
2
2
2
1
1
1
1
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Catalog No. ABIN3201396
$ 235.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1107586 EIA IHC (fro) WB Rabbit N-Term, meLys42 Log in to see Polyclonal 1
1 ABIN4995145 IHC IHC (fro) IHC (p) FITC Mouse IgG2b kappa AA 88-162, B Box Domain, full length Log in to see 19N12A1 0

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Reactivity Human
(463), (240), (168), (55), (41), (35), (24), (14), (13), (11), (11), (10), (7), (5), (3), (2), (1)
Host Mouse
(265), (215), (3), (2), (2), (2)
Clonality
Monoclonal
Conjugate This HMGB1 antibody is un-conjugated
(29), (24), (17), (17), (15), (9), (8), (8), (8), (7), (6), (6), (6), (6), (6), (5), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(411), (189), (167), (137), (118), (89), (43), (27), (26), (13), (12), (8), (6), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling Images
Specificity It has been selected for its ability to recognize HMG1 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen The antibody is a mouse monoclonal antibody raised against HMG1.
Isotype IgG
Plasmids, Primers & others

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name High Mobility Group Protein 1 (HMGB1 Antibody Abstract)
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top
Format Liquid
Concentration 500 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) antibody (ABIN3201396) anti-High-Mobility Group Box 1 (HMGB1) antibody
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) antibody (ABIN3201396) Figure.Western Blot Human Jurkat Cells Primary Ab: 3µg/mL Rabbit Anti-Human HMG1 Ab S...