anti-Human HMGB1 antibody for Immunoprecipitation

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • amphoterin
  • p30
  • hmgb1
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • hmg-1
  • hmg3
  • sbp-1
  • hmg1
  • HMGB1
  • Ac2-008
  • high mobility group box 1
  • high-mobility group box 1
  • high mobility group box 1a
  • high mobility group box 1 L homeolog
  • high mobility group protein B1
  • HMGB1
  • Hmgb1
  • hmgb1
  • hmgb1a
  • hmgb1.L
  • LOC100359149
Epitope
AA 2-215
66
59
38
38
33
16
16
15
15
15
15
14
14
11
11
11
7
6
5
5
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
443
239
168
56
40
35
24
14
13
11
11
10
7
5
3
3
1
Host
Mouse
263
196
3
2
2
2
Clonality
Monoclonal
Conjugate
This HMGB1 antibody is un-conjugated
29
24
17
15
13
8
8
7
7
7
6
6
6
6
5
5
5
4
3
2
2
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
396
185
160
131
115
95
47
26
20
13
12
8
5
3
2
2
1
1
1
1
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Catalog No. ABIN4899428
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19 ABIN2932931 IF/ICC IHC IP WB Rabbit AA 9-163 Log in to see Polyclonal 0
13.204659 ABIN516536 IP Rabbit AA 1-215, full length Log in to see Polyclonal 0
13 ABIN2932928 IF/ICC IHC IP WB Rabbit AA 1-215 Log in to see Polyclonal 0
7 ABIN6002359 IF/ICC IHC IP WB Mouse AA 1-215 Log in to see 0
1 ABIN2606382 IP Rabbit IgG AA 1-215 Log in to see Polyclonal 0
1 ABIN2606392 IP ELISA Mouse IgG1 Log in to see Moby-2 0
1 ABIN5700962 ELISA IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2694297 ChIP IF IP IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2283388 IP Rabbit IgG AA 1-215 Log in to see Polyclonal 0
1 ABIN5507264 IF IF/ICC IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN5901649 ELISA IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6031768 IF/ICC IHC IP WB Rabbit Log in to see Polyclonal 0

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope AA 2-215
(66), (59), (38), (38), (33), (16), (16), (15), (15), (15), (15), (14), (14), (11), (11), (11), (7), (6), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(443), (239), (168), (56), (40), (35), (24), (14), (13), (11), (11), (10), (7), (5), (3), (3), (1)
Host Mouse
(263), (196), (3), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HMGB1 antibody is un-conjugated
(29), (24), (17), (15), (13), (8), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(396), (185), (160), (131), (115), (95), (47), (26), (20), (13), (12), (8), (5), (3), (2), (2), (1), (1), (1), (1)
Pubmed 13 references available
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling References for anti-HMGB1 antibody (ABIN4899428) Images
Specificity Detects human HMGB1/HMG-1 in direct ELISAs and Western blots.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human HMGB1/HMG-1, Gly2-Glu215 (predicted), Accession # P09429
Clone 115603
Isotype IgG2b
Plasmids, Primers & others

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling References for anti-HMGB1 antibody (ABIN4899428) Images back to top
Antigen
Alternative Name HMGB1/HMG-1 (HMGB1 Antibody Abstract)
Background Alternative Names: Amphoterin, high mobility group box 1, High mobility group protein 1, high mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG1, HMG-1, HMG1DKFZp686A04236, HMG3, SBP-1, Sulfoglucuronyl carbohydrate binding protein
Gene ID 3146
UniProt P09429
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling References for anti-HMGB1 antibody (ABIN4899428) Images back to top
Application Notes Western Blot 0.05 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Chromatin Immunoprecipitation (ChIP) 5 μg/5 x 10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details References for anti-HMGB1 antibody (ABIN4899428) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-HMGB1 antibody (ABIN4899428)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Product cited in:

Haruma, Teshigawara, Hishikawa, Wang, Liu, Wake, Mori, Takahashi, Sugiu, Date, Nishibori: "Anti-high mobility group box-1 (HMGB1) antibody attenuates delayed cerebral vasospasm and brain injury after subarachnoid hemorrhage in rats." in: Scientific reports, Vol. 6, pp. 37755, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)).

Moser, Janik, Schiefer, Müllauer, Bekos, Scharrer, Mildner, Rényi-Vámos, Klepetko, Ankersmit: "Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology." in: PLoS ONE, Vol. 9, Issue 4, pp. e94118, 2014 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Donnelly, Errington-Mais, Steele, Hadac, Jennings, Scott, Peach, Phillips, Bond, Pandha, Harrington, Vile, Russell, Selby, Melcher: "Measles virus causes immunogenic cell death in human melanoma." in: Gene therapy, Vol. 20, Issue 1, pp. 7-15, 2013 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Liou, Adler, Keogh, Li, Jensen, Walsh, Packer, Clark, Carveth, Chen, Rogers, Lane, Moore, Sturrock, Paine, Cox, Hoidal: "Sputum biomarkers and the prediction of clinical outcomes in patients with cystic fibrosis." in: PLoS ONE, Vol. 7, Issue 8, pp. e42748, 2012 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Sperling, O?dak, Walch-Rückheim, Wickenhauser, Doorbar, Pfister, Malejczyk, Majewski, Keates, Smola: "Human papillomavirus type 8 interferes with a novel C/EBP?-mediated mechanism of keratinocyte CCL20 chemokine expression and Langerhans cell migration." in: PLoS pathogens, Vol. 8, Issue 7, pp. e1002833, 2012 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Vitali, Stronati, Negroni, Di Nardo, Pierdomenico, del Giudice, Rossi, Cucchiara: "Fecal HMGB1 is a novel marker of intestinal mucosal inflammation in pediatric inflammatory bowel disease." in: The American journal of gastroenterology, Vol. 106, Issue 11, pp. 2029-40, 2011 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Davé, Tilstra, Matsuoka, Li, DeMarco, Beer-Stolz, Sepulveda, Fink, Lotze, Plevy: "Ethyl pyruvate decreases HMGB1 release and ameliorates murine colitis." in: Journal of leukocyte biology, Vol. 86, Issue 3, pp. 633-43, 2009 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Zhou, Huang, Poon, Chen, Chan, Ng, Guan, Watt, Lu, Yuen, Zheng: "Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection." in: Journal of hepatology, Vol. 51, Issue 2, pp. 322-32, 2009 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Aychek, Miller, Sagi-Assif, Levy-Nissenbaum, Israeli-Amit, Pasmanik-Chor, Jacob-Hirsch, Amariglio, Rechavi, Witz: "E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release." in: International journal of cancer, Vol. 123, Issue 8, pp. 1741-50, 2008 Method employed by authors: Immunodepletion (IDe) (Sample species: Human).

Rowe, Jackson, Liu, Hardison, Livraghi, Solomon, McQuaid, Noerager, Gaggar, Clancy, ONeal, Sorscher, Abraham, Blalock: "Potential role of high-mobility group box 1 in cystic fibrosis airway disease." in: American journal of respiratory and critical care medicine, Vol. 178, Issue 8, pp. 822-31, 2008 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Zetterström, Jiang, Wähämaa, Ostberg, Aveberger, Schierbeck, Lotze, Andersson, Pisetsky, Erlandsson Harris: "Pivotal advance: inhibition of HMGB1 nuclear translocation as a mechanism for the anti-rheumatic effects of gold sodium thiomalate." in: Journal of leukocyte biology, Vol. 83, Issue 1, pp. 31-8, 2007 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Rao, Tracey, Pober: "IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 10, pp. 6536-46, 2007 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Seki, De Minicis, Osterreicher, Kluwe, Osawa, Brenner, Schwabe: "TLR4 enhances TGF-beta signaling and hepatic fibrosis." in: Nature medicine, Vol. 13, Issue 11, pp. 1324-32, 2007 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling References for anti-HMGB1 antibody (ABIN4899428) back to top
Supplier Images
Chromatin Immunoprecipitation (ChIP) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 2-215) antibody (ABIN4899428) Detection of HMGB1/HMG-1-regulated Genes by Chromatin Immunoprecipitation. HepG2 huma...
Flow Cytometry (FACS) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 2-215) antibody (ABIN4899428) Detection of HMGB1 in HCT-116 Human Cell Line by Flow Cytometry. HCT-116 human colore...
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 2-215) antibody (ABIN4899428) Detection of Human HMGB1/HMG-1 by Western Blot. Western blot shows lysates of HepG2 h...
Immunocytochemistry (ICC) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 2-215) antibody (ABIN4899428) HMGB1/HMG-1 in CCD-18Co Human Cell Line. HMGB1/HMG-1 was detected in immersion fixed ...