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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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1 | ABIN2621942 | IHC (p) WB | Rabbit | AA 121-170 | Log in to see | Polyclonal | 0 | |||
1 | ABIN960204 | FACS IHC (p) WB | Rabbit | AA 100-150 | Log in to see | Polyclonal | 0 |
General |
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Antigen | High-Mobility Group Box 1 (HMGB1) Antibodies |
Epitope | N-Term Alternatives |
Reactivity | Bat, Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This HMGB1 antibody is un-conjugated Alternatives |
Application |
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-HMGB1 AntibodyTarget Details HMGB1 Application Details Handling Images |
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Brand | IHC-plus™ |
Specificity | Human HMGB1 |
Purification | Immunoaffinity purified |
Immunogen |
Synthetic peptide from N-Terminus of human HMGB1 (P09429, NP_002119). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Frog (100%), Panda, Opossum (92%). Type of Immunogen: Synthetic peptide |
Target Details HMGB1Product Details anti-HMGB1 Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | HMG1 / HMGB1 (HMGB1 Antibody Abstract) |
Background |
Name/Gene ID: HMGB1 Synonyms: HMGB1, Amphoterin, High mobility group protein B1, High mobility group box 1, High mobility group protein 1, HMG1, HMG3, SBP-1, High-mobility group box 1, HMG-1 |
Gene ID | 3146 |
NCBI Accession | NP_002119 |
Pathways | p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration |
Application DetailsProduct Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top |
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Application Notes |
Approved: IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL) Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. |
Restrictions | For Research Use only |
HandlingProduct Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top |
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Format | Lyophilized |
Reconstitution | Distilled water |
Concentration | Lot specific |
Buffer | Lyophilized from PBS with 2 % sucrose |
Handling Advice | Avoid repeat freeze-thaw cycles. |
Storage | 4 °C,-20 °C |
Storage Comment |
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year) Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles. |