anti-Pig (Porcine) HMGB1 antibody for Western Blotting
Recommended HMGB1 Antibody (supplied by: Log in to see )
- Antigen
-
High-Mobility Group Box 1 (HMGB1) Antibodies
show synonyms for this antigen- HMG1
- HMG3
- SBP-1
- DEF
- HMG-1
- Hmg1
- amphoterin
- p30
- hmgb1
- ik:tdsubc_1a5
- wu:fb23c02
- xx:tdsubc_1a5
- zgc:56110
- zgc:77104
- hmg-1
- hmg3
- sbp-1
- hmg1
- HMGB1
- Ac2-008
- high mobility group box 1
- high-mobility group box 1
- high mobility group box 1a
- high mobility group box 1 L homeolog
- high mobility group protein B1
- HMGB1
- Hmgb1
- hmgb1
- hmgb1a
- hmgb1.L
- LOC100359149
- Reactivity
-
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
Alternatives - Host
-
Mouse
Alternatives - Clonality
- Conjugate
-
This HMGB1 antibody is un-conjugated
Alternatives - Application
-
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Simple Western (SimWes), Western Blotting (WB)
Alternatives - Supplier
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- Supplier Product No.
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Catalog No. ABIN4319335
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 3.0797327 | ABIN1107584 | WB | Mouse | IgG2a | Log in to see | KS1 | 1 | |||
| 3.0797327 | ABIN2957615 | WB | Rabbit | N-Term, acLys12 | Log in to see | Polyclonal | 0 | |||
| 3.0797327 | ABIN2957616 | IHC WB | Rabbit | N-Term | Log in to see | Polyclonal | 0 | |||
| 3.0797327 | ABIN2957614 | IC IF IHC WB | Rabbit | Log in to see | Polyclonal | 0 | ||||
| 3.0797327 | ABIN1580427 | ICC IF IHC WB | Mouse | IgG2b | Log in to see | 1F3 | 0 | |||
| 1 | ABIN324443 | WB | Rabbit | IgG | AA 126-175 | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN203033 | WB | Rabbit | C-Term | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN575653 | WB | Rabbit | AA 21-70 | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN615020 | IHC (p) WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN1735506 | IHC (p) WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN2621941 | IHC IHC (p) WB | Rabbit | N-Term | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN2621942 | IHC IHC (p) WB | Rabbit | AA 121-170 | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN208594 | WB | Mouse | IgG2a | Log in to see | 0 | ||||
| 1 | ABIN4319323 | IHC (p) WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN2467469 | IHC WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN4961214 | FACS ICC IF WB | Alexa Fluor 700 | Mouse | IgG2b | Log in to see | 1F3 | 0 | ||
| 1 | ABIN4961226 | FACS ICC IF WB | DyLight 350 | Mouse | IgG2b | Log in to see | 1F3 | 0 | ||
| 1 | ABIN4961216 | ICC IF WB | Biotin | Mouse | IgG2b | Log in to see | 1F3 | 0 | ||
| 1 | ABIN4961211 | FACS ICC IF WB | Alexa Fluor 405 | Mouse | IgG2b | Log in to see | 1F3 | 0 | ||
| 1 | ABIN4961220 | FACS ICC IF WB | DyLight 488 | Mouse | IgG2b | Log in to see | 1F3 | 0 |
General |
|
|---|---|
| Antigen | High-Mobility Group Box 1 (HMGB1) Antibodies |
| Reactivity | Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus) Alternatives |
| Host | Mouse Alternatives |
| Clonality (Clone) | |
| Conjugate | This HMGB1 antibody is un-conjugated Alternatives |
| Application |
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Simple Western (SimWes), Western Blotting (WB)
Alternatives
|
| Supplier | Log in to see |
Product Details anti-HMGB1 AntibodyTarget Details HMGB1 Application Details Handling Images |
|
| Purification | Immunogen affinity purified |
| Immunogen | Full length human HMGB1 [UniProt# P09429] |
| Clone | 1F3 |
| Isotype | IgG2b |
| Plasmids, Primers & others | |
Target Details HMGB1Product Details anti-HMGB1 Antibody Application Details Handling Images back to top |
|
| Antigen | |
| Alternative Name | HMGB1/HMG-1 (HMGB1 Antibody Abstract) |
| Background | Gene Symbol: HMGB1 |
| Molecular Weight | Theoretical MW: 25 kDa |
| Gene ID | 3146 |
| UniProt | P09429 |
| Pathways | p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration |
Application DetailsProduct Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top |
|
| Application Notes | Western Blot 1:1000-1:2000, Simple Western 1:500, Flow Cytometry 1 μg per million cells, Immunocytochemistry/Immunofluorescence 1:1000, CyTOF-readyThis HMGB1 (1F3) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen at approx. 25 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
| Restrictions | For Research Use only |
HandlingProduct Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top |
|
| Format | Liquid |
| Concentration | 1 mg/mL |
| Buffer |
PBS Buffer contains: 10 mM Sodium Azide |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Storage | 4 °C,-20 °C |
| Storage Comment | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
ImagesProduct Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top |
|
| Supplier Images |
Simple Western: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Simple Western lane view sh...
Western Blot: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Blot of crude HeLa cell extra...
Flow Cytometry: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Intracellular flow cytometr...
Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - HeL...
Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) - Analysis of of ...
Western Blot: HMGB1/HMG-1 Antibody (1F3) - Analysis of lysates from different cell l...
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