anti-Rat (Rattus) HMGB1 antibody for Immunofluorescence

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • amphoterin
  • p30
  • hmgb1
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • hmg-1
  • hmg3
  • sbp-1
  • hmg1
  • HMGB1
  • Ac2-008
  • high mobility group box 1
  • high-mobility group box 1
  • high mobility group box 1a
  • high mobility group box 1 L homeolog
  • high mobility group protein B1
  • HMGB1
  • Hmgb1
  • hmgb1
  • hmgb1a
  • hmgb1.L
  • LOC100359149
Epitope
Center
61
58
38
38
34
20
18
18
16
15
15
14
13
11
11
9
7
5
5
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
455
250
177
61
40
38
24
14
13
11
11
10
7
5
3
3
1
Host
Rabbit
273
197
3
2
2
2
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
31
24
17
16
14
8
8
7
7
7
7
7
7
6
5
5
5
4
3
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
410
188
164
132
118
95
47
26
21
13
12
6
6
4
2
2
1
1
1
1
1
Options
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Available images

Catalog No. ABIN2854997
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.64368 ABIN4903942 FACS ICC IF IHC WB Rabbit IgG Log in to see 0
23.593393 ABIN250703 ELISA FACS ICC IF IHC IHC (p) SimWes WB Rabbit AA 100-200, Internal Region Log in to see Polyclonal 15
23.5 ABIN6269456 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 5
20.5 ABIN6262301 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 1
19 ABIN2506663 ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
19 ABIN1873041 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
18.02734 ABIN3185048 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
17.64368 ABIN6569270 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
14.643681 ABIN2957614 IC IF IHC WB Rabbit Log in to see Polyclonal 0
14.593393 ABIN4319335 CyTOF FACS ICC IF SimWes WB Mouse IgG2b Log in to see 1F3 1
11.643681 ABIN1580427 ICC IF IHC WB Mouse IgG2b Log in to see 1F3 0
8.5 ABIN2474000 FACS IF WB Hamster IgG Log in to see HMG1-5H6 1
7 ABIN2862626 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN6258233 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN1735426 ICC FACS IF IHC WB Rabbit AA 100-200 Log in to see Polyclonal 0
4 ABIN272158 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
4 ABIN5959559 ELISA IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN1959851 ICC IF WB Mouse IgG2b Log in to see 1F3 0
4 ABIN6712351 IF IHC WB Rabbit AA 131-180 Log in to see Polyclonal 0
4 ABIN6715943 IF IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope Center
(61), (58), (38), (38), (34), (20), (18), (18), (16), (15), (15), (14), (13), (11), (11), (9), (7), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(455), (250), (177), (61), (40), (38), (24), (14), (13), (11), (11), (10), (7), (5), (3), (3), (1)
Host Rabbit
(273), (197), (3), (2), (2), (2), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(31), (24), (17), (16), (14), (8), (8), (7), (7), (7), (7), (7), (7), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(410), (188), (164), (132), (118), (95), (47), (26), (21), (13), (12), (6), (6), (4), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling Images
Cross-Reactivity Dog (Canine), Pig (Porcine), Rabbit, Rhesus Monkey, Cow (Bovine)
Cross-Reactivity (Details) Dog (100 %), Pig (100 %), Rabbit (100 %), Rhesus Monkey (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to HMGB1 (high-mobility group box 1)
HMGB1 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HMGB1 (HMGB1 Antibody Abstract)
Background DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Cellular Localization: Nucleus
Molecular Weight 25 kDa
Gene ID 3146
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* Immunohistochemistry Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* ImmunohistochemistryAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , HepG2 , A375 , NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12

Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top
Format Liquid
Concentration 1.92 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-High-Mobility Group Box 1 (HMGB1) (Center) antibody (ABIN2854997) IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on mouse esophagus by imm...
Immunofluorescence (IF) image for anti-High-Mobility Group Box 1 (HMGB1) (Center) antibody (ABIN2854997) ICC/IF Image HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent ana...
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (Center) antibody (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by western blot analysis. Various whole...
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (Center) antibody (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by Western blot analysis. A. 30 µg NIH-...
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (Center) antibody (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by Western blot analysis. A. 30 µg PC-1...
Immunohistochemistry (IHC) image for anti-High-Mobility Group Box 1 (HMGB1) (Center) antibody (ABIN2854997) IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on rat brain stem by immu...
Immunofluorescence (IF) image for anti-High-Mobility Group Box 1 (HMGB1) (Center) antibody (ABIN2854997) ICC/IF Image HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent ana...
Immunohistochemistry (IHC) image for anti-High-Mobility Group Box 1 (HMGB1) (Center) antibody (ABIN2854997) IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on mouse colon by immunoh...
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (Center) antibody (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by Western blot analysis. A. 30 µg 293T...
Immunohistochemistry (IHC) image for anti-High-Mobility Group Box 1 (HMGB1) (Center) antibody (ABIN2854997) IHC-P Image Immunohistochemical analysis of paraffin-embedded 59T xenograft, using HM...