anti-Zebrafish (Danio rerio) HMGB1 antibody for Western Blotting

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • amphoterin
  • p30
  • hmgb1
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • hmg-1
  • hmg3
  • sbp-1
  • hmg1
  • HMGB1
  • Ac2-008
  • high mobility group box 1
  • high-mobility group box 1
  • high mobility group box 1a
  • high mobility group box 1 L homeolog
  • high mobility group protein B1
  • HMGB1
  • Hmgb1
  • hmgb1
  • hmgb1a
  • hmgb1.L
  • LOC100359149
Epitope
N-Term
69
64
38
38
33
22
19
19
15
15
14
14
14
11
11
10
7
6
5
5
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
457
240
167
54
41
34
23
14
13
10
10
10
7
5
2
2
1
Host
Rabbit
264
210
3
2
2
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
29
24
17
17
15
9
8
8
8
7
6
6
6
6
6
5
5
5
4
3
3
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
406
190
168
137
118
89
44
26
22
13
12
8
6
3
2
2
1
1
1
1
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Catalog No. ABIN2787911
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.8724732 ABIN2780400 WB Rabbit Middle Region Log in to see Polyclonal 1
1 ABIN5689862 WB Rabbit N-Term Log in to see Polyclonal 0

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope N-Term
(69), (64), (38), (38), (33), (22), (19), (19), (15), (15), (14), (14), (14), (11), (11), (10), (7), (6), (5), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(457), (240), (167), (54), (41), (34), (23), (14), (13), (10), (10), (10), (7), (5), (2), (2), (1)
Host Rabbit
(264), (210), (3), (2), (2), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(29), (24), (17), (17), (15), (9), (8), (8), (8), (7), (6), (6), (6), (6), (6), (5), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(406), (190), (168), (137), (118), (89), (44), (26), (22), (13), (12), (8), (6), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN978053
Sequence QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against HMGB1. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HMGB1
Plasmids, Primers & others

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HMGB1 (HMGB1 Antibody Abstract)
Background Extracellular HMGB1 is an activator of human tumor cell migration operating in concert with EGF. HMGB1 encodes a protein that is potentially involved in the regulation of lipogenic and cholesterogenic gene transcription.
Alias Symbols: DKFZp686A04236, HMG1, HMG3, SBP-1
Protein Interaction Partner: AGTRAP, HUWE1, UBC, ASB2, ASB1, ZNF24, MAPK1, HMGA1, ITGA4, FN1, HNF1A, SSRP1, SMARCE1, PSEN1, XPC, XPA, RAD23B, CDK2, TFAP4, TERF2IP, TERF2, HHV8GK18_gp81, SUMO2, FCP1, DNM2, HSPA8, HMGB2, PDIA3, Ccdc15, POU5F1, H3F3A, CREBBP, CDK1, TBP, LRIF1, RASSF4, N
Protein Size: 215
Molecular Weight 25 kDa
Gene ID 3146
NCBI Accession NM_002128, NP_002119
UniProt P09429
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 215 AA

Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (N-Term) antibody (ABIN2787911) WB Suggested Anti-HMGB1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (N-Term) antibody (ABIN2787911) WB Suggested Anti-HMGB1
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:1562500...