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|Antigen||K(lysine) Acetyltransferase 2B (KAT2B) Antibodies|
|Epitope||AA 817-832 Alternatives|
|Reactivity||Bat, Cow (Bovine), Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine) Alternatives|
|Conjugate||This KAT2B antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-KAT2B AntibodyTarget Details KAT2B Application Details Handling Images
|Specificity||Recognizes human and canine PCAF. Species cross-reactivity: Predicted to react with mouse and chicken due to sequence homology.|
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse (100%) Rat, Opossum, Platypus, Xenopus (94%) Chicken, Lizard, Zebrafish (88%) Stickleback, Pufferfish (81%).|
Synthetic peptide [EKFFFSKIKEAGLIDK] (with cysteine added to N-terminal) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of human PCAF. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse (100%), Rat, Opossum, Platypus, Xenopus (94%), Chicken, Lizard, Zebrafish (88%), Stickleback, Pufferfish (81%).
Type of Immunogen: Synthetic peptide - KLH conjugated
Target Details KAT2BProduct Details anti-KAT2B Antibody Application Details Handling Images back to top
|Alternative Name||KAT2B / PCAF (KAT2B Antibody Abstract)|
Name/Gene ID: KAT2B
Synonyms: KAT2B, CREBBP-associated factor, GCN5, Histone acetyltransferase PCAF, K(lysine) acetyltransferase 2B, Lysine acetyltransferase 2B, Histone acetylase PCAF, PCAF, p300/CBP-associated factor, CAF, GCN5L
|Pathways||p53 Signaling, Regulation of Carbohydrate Metabolic Process|
Application DetailsProduct Details anti-KAT2B Antibody Target Details KAT2B Handling Images back to top
Approved: IP, WB (1:500)
Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:500, Determined using whole extracts of human epidermoid carcinoma A431, canine MDCK kidney cells or recombinant active human PCAF, and a chemiluminescent detection reagent. Predicted molecular weight: ~88kD. In some extract preparations, additional band (s) may appear. Staining of PCAF band (s) by immunoblotting is inhibited by the PCAF immunizing peptide. Immunoprecipitation: 5 μg immunoprecipitates PCAF from RIPA lysate with ~4x10^7 human acute T cell leukemia Jurkat cells.
|Restrictions||For Research Use only|
HandlingProduct Details anti-KAT2B Antibody Target Details KAT2B Application Details Images back to top
|Buffer||PBS, pH 7.4, 1 % BSA, 15 mM sodium azide, 40 % glycerol.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.