anti-Human p300 antibody for Immunohistochemistry

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • KAT3B
  • RSTS2
  • p300
  • A430090G16
  • A730011L11
  • p300 HAT
  • E1A binding protein p300
  • histone acetyltransferase p300
  • EP300
  • LOC446056
  • ep300
  • Ep300
Epitope
pSer1834
57
43
39
19
18
11
6
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
269
125
111
38
37
3
3
3
3
3
2
Host
Rabbit
182
98
Clonality
Polyclonal
Conjugate
This p300 antibody is un-conjugated
11
11
11
8
8
6
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
192
112
96
74
56
48
47
38
28
11
7
5
3
2
2
1
1
1
1
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Catalog No. ABIN6271336
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.767978 ABIN3186252 ELISA IHC WB Rabbit IgG Ser342 Log in to see Polyclonal 0
15.767978 ABIN3186253 ELISA IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
13.004274 ABIN2957697 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal 0
13.004274 ABIN1848716 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
12.767978 ABIN3182837 ELISA IHC Rabbit IgG pSer89 Log in to see Polyclonal 0
12.767978 ABIN5618473 ELISA IHC Rabbit IgG pSer89 Log in to see Polyclonal 0
12.767978 ABIN5620431 IHC WB Rabbit N-Term Log in to see Polyclonal 0
12.767978 ABIN2140669 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 0
10.004274 ABIN2707672 IHC WB Rabbit N-Term Log in to see Polyclonal 0
10.004274 ABIN1533367 IHC ELISA WB Rabbit IgG AA 1-50 Log in to see Polyclonal 0
10.004274 ABIN6263955 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
10.004274 ABIN6263956 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
10.004274 ABIN790522 ELISA IF IHC IHC (p) WB Mouse IgG1 AA 731-831 Log in to see 1B1 0
10.004274 ABIN790523 ELISA IF IHC IHC (p) WB Mouse IgG1 AA 731-831 Log in to see 1D2 0
10.004274 ABIN2957698 IHC WB Rabbit Log in to see Polyclonal 0
10.004274 ABIN5577325 IF IHC Rabbit IgG Log in to see Polyclonal 0
10.004274 ABIN3001773 DB IHC Rabbit IgG pSer89 Log in to see Polyclonal 0
10.004274 ABIN2434592 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
10.004274 ABIN2432981 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
7.004275 ABIN1531355 IHC ELISA Rabbit IgG AA 55-104, pSer89 Log in to see Polyclonal 1

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Epitope pSer1834
(57), (43), (39), (19), (18), (11), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(269), (125), (111), (38), (37), (3), (3), (3), (3), (3), (2)
Host Rabbit
(182), (98)
Clonality
Polyclonal
Conjugate This p300 antibody is un-conjugated
(11), (11), (11), (8), (8), (6), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(192), (112), (96), (74), (56), (48), (47), (38), (28), (11), (7), (5), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling Images
Specificity E1A-associated Protein p300 (Phospho-Ser1834) Antibody detects endogenous levels of E1A-associated Protein p300 only when phosphorylated at Ser1834
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human E1A-associated Protein p300 (Phospho-Ser1834)
Isotype IgG
Plasmids, Primers & others

Target Details p300

Product Details anti-p300 Antibody Application Details Handling Images back to top
Antigen
Alternative Name E1A-associated Protein p300 (EP300 Antibody Abstract)
Background

Description: Functions as histone acetyltransferase and regulates transcription via chromatin remodeling (PubMed:23415232, PubMed:23934153, PubMed:8945521). Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation (PubMed:23415232, PubMed:23934153, PubMed:8945521). Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac) (PubMed:23911289). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator (PubMed:12929931). Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function (PubMed:18722353). Acetylates HDAC1 leading to its inactivation and modulation of transcription (PubMed:16762839). Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2 (PubMed:12586840). Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Acetylates FOXO1 and enhances its transcriptional activity (PubMed:15890677). Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity (PubMed:12402037). Participates in CLOCK or NPAS2-regulated rhythmic gene transcription, exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter (PubMed:14645221). Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:16617102). Acetylates XBP1 isoform 2, acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA, acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). Acetylates MEF2D (PubMed:21030595). Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degragation, this mechanism may be involved in CD4/CD8 lineage differentiation (PubMed:20810990). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA) or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation or propionylation, respectively (PubMed:25818647, PubMed:17267393). Acts as a histone crotonyltransferase, crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors (PubMed:25818647). Histone crotonyltransferase activity is dependent on the concentration of (2E)-butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (E)-but-2-enoyl-CoA (crotonyl-CoA) concentration is low (PubMed:25818647). Also acts as a histone butyryltransferase, butyrylation marks active promoters (PubMed:17267393).

Gene: EP300

Molecular Weight 264kDa
Gene ID 2033
UniProt Q09472
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling Images back to top
Application Notes WB 1:1000-3000, IHC 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-E1A Binding Protein P300 (EP300) (pSer1834) antibody (ABIN6271336) ABIN6271337 at 1/200 staining Human breast tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-E1A Binding Protein P300 (EP300) (pSer1834) antibody (ABIN6271336) ABIN6271337 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was for...
Immunohistochemistry (IHC) image for anti-E1A Binding Protein P300 (EP300) (pSer1834) antibody (ABIN6271336) ABIN6271337 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was for...
Western Blotting (WB) image for anti-E1A Binding Protein P300 (EP300) (pSer1834) antibody (ABIN6271336) Western blot analysis of E1A-associated Protein p300 (Phospho-Ser1834) using PMA trea...