anti-Cow (Bovine) p53 antibody for Immunocytochemistry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • transformation related protein 53, pseudogene
  • Wistar clone pR53P1 p53 pseudogene
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
  • Trp53-ps
  • p53-ps
Epitope
AA 20-25
173
128
90
82
73
71
70
61
58
54
49
48
48
47
41
38
32
30
28
23
22
20
17
16
15
15
15
15
15
15
14
12
11
11
10
10
9
9
9
8
8
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Primate
2381
517
428
190
126
93
87
84
35
23
20
19
16
16
15
14
6
4
4
4
4
3
3
3
2
2
1
1
Host
Mouse
1354
1138
7
3
1
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
81
78
65
54
47
46
45
31
31
26
25
25
25
25
25
24
24
24
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1907
770
742
738
630
622
434
344
316
179
72
41
32
31
21
17
17
15
13
9
9
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4342819
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4342821 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342820 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342822 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342818 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342817 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342816 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2b kappa AA 37-45 Log in to see DO-7
1 ABIN4342835 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342836 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342837 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342852 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342855 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342859 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342858 FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342860 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342865 FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342867 FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342870 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG2b kappa AA 37-45 Log in to see DO-7
1 ABIN4342849 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342857 ICC IF IHC (fro) IHC (p) WB Biotin Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342862 FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG AA 20-25 Log in to see BP53-12-DO-7

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 20-25
(173), (128), (90), (82), (73), (71), (70), (61), (58), (54), (49), (48), (48), (47), (41), (38), (32), (30), (28), (23), (22), (20), (17), (16), (15), (15), (15), (15), (15), (15), (14), (12), (11), (11), (10), (10), (9), (9), (9), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Primate
(2381), (517), (428), (190), (126), (93), (87), (84), (35), (23), (20), (19), (16), (16), (15), (14), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(1354), (1138), (7), (3), (1), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(81), (78), (65), (54), (47), (46), (45), (31), (31), (26), (25), (25), (25), (25), (25), (24), (24), (24), (20), (19), (18), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1907), (770), (742), (738), (630), (622), (434), (344), (316), (179), (72), (41), (32), (31), (21), (17), (17), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling Images
Specificity Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. Its epitope maps within the N-terminus (aa 20-25) of p53 oncoprotein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Purification Protein A purified
Immunogen Recombinant human wild-type p53 protein (BP53-12) & Recombinant human wild type p53 protein expressed in E. coli (DO-7)
Clone BP53-12-DO-7
Isotype IgG

Target Details p53

Product Details anti-p53 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background Gene Symbol: TP53
Molecular Weight Theoretical MW: 53 kDa
Gene ID 7157
UniProt P04637
Research Area DNA/RNA, Cell Cycle, Transcription Factors, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling Images back to top
Application Notes Western Blot 0.5-1.0 μg/mL, Flow Cytometry 0.5-1 μg/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 μg/mL, Immunoprecipitation 0.5-1 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mLHuman Chromosome Location: 17p13.1 Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.