anti-Cow (Bovine) p53 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
Reactivity
Cow (Bovine), Human, Monkey
2647
572
481
302
154
120
119
87
52
23
22
19
19
16
14
13
6
5
4
4
3
3
3
1
1
Host
Mouse
1492
1282
7
1
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is conjugated to CF®405S
88
85
74
61
54
46
45
35
32
31
31
25
25
25
25
24
24
24
20
19
18
18
18
14
14
14
14
14
12
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
5
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
2175
974
893
803
781
752
425
348
311
179
109
90
75
41
34
18
17
15
13
13
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN6164587
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6164595 FACS IF IHC (f) WB CF®647 Mouse IgG2b, kappa Log in to see DO-7 0
1 ABIN6164519 FACS IF IHC (f) WB CF®647 Mouse IgG Log in to see BP53-12 DO-7 0
1 ABIN6164525 FACS IF IHC (f) WB CF®568 Mouse IgG Log in to see BP53-12 DO-7 0
1 ABIN6164515 FACS IF IHC (f) WB CF®640R Mouse IgG Log in to see BP53-12 DO-7 0
1 ABIN6164517 FACS IF IHC (f) WB CF®543 Mouse IgG Log in to see BP53-12 DO-7 0
1 ABIN6164511 FACS IF IHC (f) WB CF®405S Mouse IgG Log in to see BP53-12 DO-7 0
1 ABIN6164513 FACS IF IHC (f) WB CF405M Mouse IgG Log in to see BP53-12 DO-7 0
1 ABIN6164523 FACS IF IHC (f) WB CF®660R Mouse IgG Log in to see BP53-12 DO-7 0
1 ABIN6164521 FACS IF IHC (f) WB CF®555 Mouse IgG Log in to see BP53-12 DO-7 0
1 ABIN6164531 FACS IF IHC (f) WB CF®680R Mouse IgG Log in to see BP53-12 DO-7 0
1 ABIN6164529 FACS IF IHC (f) WB CF®680 Mouse IgG Log in to see BP53-12 DO-7 0
1 ABIN6164533 FACS IF IHC (f) WB CF®488A Mouse IgG Log in to see BP53-12 DO-7 0
1 ABIN6164599 FACS IF IHC (f) WB CF®660R Mouse IgG2b, kappa Log in to see DO-7 0
1 ABIN6164609 FACS IF IHC (f) WB CF®488A Mouse IgG2b, kappa Log in to see DO-7 0
1 ABIN6164589 FACS IF IHC (f) WB CF405M Mouse IgG2b, kappa Log in to see DO-7 0
1 ABIN6164593 FACS IF IHC (f) WB CF®543 Mouse IgG2b, kappa Log in to see DO-7 0
1 ABIN6164591 FACS IF IHC (f) WB CF®640R Mouse IgG2b, kappa Log in to see DO-7 0
1 ABIN6164601 FACS IF IHC (f) WB CF®568 Mouse IgG2b, kappa Log in to see DO-7 0
1 ABIN6164597 FACS IF IHC (f) WB CF®555 Mouse IgG2b, kappa Log in to see DO-7 0
1 ABIN6164603 FACS IF IHC (f) WB CF®770 Mouse IgG2b, kappa Log in to see DO-7 0

General

Antigen Tumor Protein P53 (TP53) Antibodies
Reactivity Cow (Bovine), Human, Monkey
(2647), (572), (481), (302), (154), (120), (119), (87), (52), (23), (22), (19), (19), (16), (14), (13), (6), (5), (4), (4), (3), (3), (3), (1), (1)
Host Mouse
(1492), (1282), (7), (1), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is conjugated to CF®405S
(88), (85), (74), (61), (54), (46), (45), (35), (32), (31), (31), (25), (25), (25), (25), (24), (24), (24), (20), (19), (18), (18), (18), (14), (14), (14), (14), (14), (12), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (5), (2), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(2175), (974), (893), (803), (781), (752), (425), (348), (311), (179), (109), (90), (75), (41), (34), (18), (17), (15), (13), (13), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling Images
Purpose Mouse Monoclonal anti-p53 (DO-7), CF405S Conjugate
Characteristics This antibody recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 37-45) of p53. Monoclonal antibody PAb1801 does not block the binding of DO-7 MAb to p53 in an ELISA test. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant human wild type p53 protein expressed in E. coli.
Clone DO-7
Isotype IgG2b, kappa
Plasmids, Primers & others

Target Details p53

Product Details anti-p53 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53
Molecular Weight 53 kDa
Gene ID 7157, 654481
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.25-0.5 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Immunofluorescence 0.5-1 μg/mL
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Western blotting 0.5-1.0 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

MDA-MB-231 Cells. Breast or Colon carcinoma

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light