anti-Cow (Bovine) p53 antibody for Immunoprecipitation

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
Epitope
AA 14-289
185
119
89
87
76
75
70
60
58
55
51
46
46
44
42
34
34
34
25
24
22
22
22
22
17
15
15
15
15
15
15
15
14
13
12
11
11
11
10
9
9
8
8
8
8
7
7
7
7
7
7
7
7
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
2648
587
493
300
156
119
119
87
53
24
22
21
21
16
16
15
6
5
5
4
4
3
3
2
2
2
1
1
Host
Mouse
1500
1271
7
3
1
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
88
85
74
61
54
46
45
35
32
31
31
25
25
25
25
24
24
24
20
19
18
18
18
14
14
14
14
14
12
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
5
2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
2180
980
904
813
772
739
425
363
305
179
110
90
75
41
32
18
17
15
13
13
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN967420
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967419 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 14-289 Log in to see PAb 240 7
1 ABIN320175 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 4
1 ABIN120054 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 3
1 ABIN2476020 ELISA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 4
1 ABIN385146 FACS IF IHC IHC (p) IP WB Mouse IgG2b, kappa Log in to see DO-7 0
1 ABIN4342819 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7 0
1 ABIN4342822 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342816 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2b kappa AA 37-45 Log in to see DO-7 0
1 ABIN4342840 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342846 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342824 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342828 CyTOF FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7 0
1 ABIN4342830 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see DO-7 0
1 ABIN198280 ELISA IHC IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 0
1 ABIN4342844 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB HRP Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN2476024 FACS IHC (fro) IHC (p) IP WB Mouse IgG2b AA 20-25 Log in to see DO-7 4
1 ABIN198282 FACS IHC IHC (fro) IHC (p) IP WB Mouse IgG2b AA 20-25 Log in to see DO-7 0
1 ABIN385155 FACS IF IHC IHC (p) IP WB Biotin Mouse IgG2b, kappa Log in to see DO-7 0
1 ABIN2745094 ELISA FACS IF IHC IHC (p) IP WB Mouse IgG2b, kappa Log in to see DO-7 0
1 ABIN1818245 IP Mouse IgG1 AA 310-325, pSer315 Log in to see FPS315 0

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 14-289
(185), (119), (89), (87), (76), (75), (70), (60), (58), (55), (51), (46), (46), (44), (42), (34), (34), (34), (25), (24), (22), (22), (22), (22), (17), (15), (15), (15), (15), (15), (15), (15), (14), (13), (12), (11), (11), (11), (10), (9), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
(2648), (587), (493), (300), (156), (119), (119), (87), (53), (24), (22), (21), (21), (16), (16), (15), (6), (5), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Host Mouse
(1500), (1271), (7), (3), (1), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(88), (85), (74), (61), (54), (46), (45), (35), (32), (31), (31), (25), (25), (25), (25), (24), (24), (24), (20), (19), (18), (18), (18), (14), (14), (14), (14), (14), (12), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (2), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(2180), (980), (904), (813), (772), (739), (425), (363), (305), (179), (110), (90), (75), (41), (32), (18), (17), (15), (13), (13), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 antibody (ABIN967420) Images
Brand BD Pharmingen™
Cross-Reactivity Mouse (Murine), Rat (Rattus), Hamster, Monkey, Cow (Bovine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human p53 aa. 14-289
Clone PAb 240
Isotype IgG1
Plasmids, Primers & others

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 antibody (ABIN967420) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background P53 is a nuclear phosphoprotein which acts as a tumor suppressor by providing a cell cycle checkpoint for DNA damage during S-phase. Mutations in wildtype p53 can indirectly alter the DNA binding and transcription factor activity of p53. By altering expression of genes normally regulated by p53, these mutations can result in both a loss of tumor suppressor function and a gain of oncogenic function. The majority of mutations in the p53 gene are missense mutations which alter the identity of an amino acid. These mutations may alter the conformation and thus increase the stability of the mutant p53 protein. p53 is expressed in all vertebrate species examined. p53 may be overexpressed in transformed cell lines, where it forms complexes with viral oncogenes including SV40 large T antigen and the adenovirus protein, E1B. p53 migrates at ~53 kDa on SDS-PAGE.
PAb 240 reacts with a conformational epitope between amino acids 156 and 214 of native p53. As such, Pab 240 recognizes only certain mutant forms of p53, as determined by immunoprecipitation. It detects both mutant and wildtype p53 in western blot analysis and immunohistochemistry of frozen tissue sections. It is thought that p53 mutations exert a common conformational change which results in expression of the Pab 240-specific epitope on mutant p53 molecules. A recombinant fusion protein of p53 sequence including amino acids 14-289 was used as immunogen.
Molecular Weight 53 kDa
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 antibody (ABIN967420) Images back to top
Application Notes SV40 transformed COS-7 monkey kidney cells (ATCC CRL-1651) or other SV40-transformed cell lines are suggested as positive controls.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 antibody (ABIN967420) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-p53 antibody (ABIN967420)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Jacquemier, Molès, Penault-Llorca, Adélaide, Torrente, Viens, Birnbaum, Theillet: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, 1994

Said, Barrera, Shintaku, Nakamura, Koeffler: "Immunohistochemical analysis of p53 expression in malignant lymphomas." in: The American journal of pathology, Vol. 141, Issue 6, pp. 1343-8, 1993

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992

Moore, Teresky, Levine, Seiberg: "p53 mutations are not selected for in simian virus 40 T-antigen-induced tumors from transgenic mice." in: Journal of virology, Vol. 66, Issue 2, pp. 641-9, 1992

Walker, Dearing, Lane, Varley: "Expression of p53 protein in infiltrating and in-situ breast carcinomas." in: The Journal of pathology, Vol. 165, Issue 3, pp. 203-11, 1992

Bártek, Bártková, Vojt?sek, Stasková, Lukás, Rejthar, Kovarík, Midgley, Gannon, Lane: "Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies." in: Oncogene, Vol. 6, Issue 9, pp. 1699-703, 1991

Gannon, Greaves, Iggo, Lane: "Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form." in: The EMBO journal, Vol. 9, Issue 5, pp. 1595-602, 1990

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 antibody (ABIN967420) back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 14-289) antibody (ABIN967420) Western blot analysis of p53. Lysate from SV40-transformed COS-7 cells was probed wit...