anti-Dog (Canine) p53 antibody for Flow Cytometry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
Reactivity
Chicken, Dog (Canine), Hamster, Human, Monkey
2533
568
476
307
162
103
102
67
55
26
23
23
18
17
17
16
7
6
5
4
4
4
4
3
3
2
2
1
1
Host
Mouse
1483
1166
7
5
1
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is conjugated to PerCP
82
81
69
52
45
38
37
32
23
23
22
20
20
20
20
20
19
19
18
18
17
16
15
14
14
14
14
14
12
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
5
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
2088
962
864
782
681
663
421
317
267
179
109
90
75
41
32
17
17
15
13
10
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN6164582
$ 392.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN6164583 FACS IF IHC (f) WB PE Mouse IgG2a Log in to see BP53-12 0
7 ABIN6164575 FACS IF IHC (f) WB APC Mouse IgG2a Log in to see BP53-12 0
7 ABIN6164586 FACS IF IHC (f) WB Mouse IgG2a Log in to see BP53-12 0
1 ABIN6164641 FACS IF IHC (fro) CF®770 Mouse IgG2b, kappa Log in to see PAb122 0
1 ABIN6164637 FACS IF IHC (fro) CF®660R Mouse IgG2b, kappa Log in to see PAb122 0
1 ABIN6164647 FACS IF IHC (fro) CF®488A Mouse IgG2b, kappa Log in to see PAb122 0
1 ABIN6164649 FACS IF IHC (fro) CF®594 Mouse IgG2b, kappa Log in to see PAb122 0
1 ABIN6164555 FACS IF IHC (f) WB CF®543 Mouse IgG2a Log in to see BP53-12 0
1 ABIN6164565 FACS IF IHC (f) WB CF®770 Mouse IgG2a Log in to see BP53-12 0
1 ABIN6164645 FACS IF IHC (fro) CF®680R Mouse IgG2b, kappa Log in to see PAb122 0
1 ABIN6164625 FACS IF IHC (fro) CF®405S Mouse IgG2b, kappa Log in to see PAb122 0
1 ABIN6164629 FACS IF IHC (fro) CF®640R Mouse IgG2b, kappa Log in to see PAb122 0
1 ABIN6164639 FACS IF IHC (fro) CF®568 Mouse IgG2b, kappa Log in to see PAb122 0
1 ABIN6164557 FACS IF IHC (f) WB CF®647 Mouse IgG2a Log in to see BP53-12 0
1 ABIN6164553 FACS IF IHC (f) WB CF®640R Mouse IgG2a Log in to see BP53-12 0
1 ABIN6164561 FACS IF IHC (f) WB CF®660R Mouse IgG2a Log in to see BP53-12 0
1 ABIN6164643 FACS IF IHC (fro) CF®680 Mouse IgG2b, kappa Log in to see PAb122 0
1 ABIN6164563 FACS IF IHC (f) WB CF®568 Mouse IgG2a Log in to see BP53-12 0
1 ABIN6164573 FACS IF IHC (f) WB CF®594 Mouse IgG2a Log in to see BP53-12 0
1 ABIN6164549 FACS IF IHC (f) WB CF®405S Mouse IgG2a Log in to see BP53-12 0

General

Antigen Tumor Protein P53 (TP53) Antibodies
Reactivity Chicken, Dog (Canine), Hamster, Human, Monkey
(2533), (568), (476), (307), (162), (103), (102), (67), (55), (26), (23), (23), (18), (17), (17), (16), (7), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1)
Host Mouse
(1483), (1166), (7), (5), (1), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is conjugated to PerCP
(82), (81), (69), (52), (45), (38), (37), (32), (23), (23), (22), (20), (20), (20), (20), (20), (19), (19), (18), (18), (17), (16), (15), (14), (14), (14), (14), (14), (12), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (2), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(2088), (962), (864), (782), (681), (663), (421), (317), (267), (179), (109), (90), (75), (41), (32), (17), (17), (15), (13), (10), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling Images
Purpose Mouse Monoclonal anti-p53 (BP53-12), Per-CP Conjugate
Characteristics This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 Molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70 % but incidence varies from zero in carcinoid lung tumors to 97 % in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant human wild-type p53 protein
Clone BP53-12
Isotype IgG2a
Plasmids, Primers & others

Target Details p53

Product Details anti-p53 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53
Molecular Weight 53 kDa
Gene ID 7157, 654481
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling Images back to top
Application Notes Does not react with mouse or rat, others not known
  • Immunohistology formalin-fixed 0.5-1 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Western blotting 0.5-1 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

MDA-MB-231 Cells. Breast or Colon carcinoma

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling back to top
Supplier Images
 image for anti-Tumor Protein P53 (TP53) antibody (PerCP) (ABIN6164582) anti-Tumor Protein P53 (TP53) antibody (PerCP)
 image for anti-Tumor Protein P53 (TP53) antibody (PerCP) (ABIN6164582) anti-Tumor Protein P53 (TP53) antibody (PerCP) (Image 2)