anti-Human p53 antibody for Immunocytochemistry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
Reactivity
Human
2533
568
476
308
162
104
103
67
56
26
23
23
18
17
17
16
7
6
5
4
4
4
4
3
3
2
2
1
1
Host
Mouse
1483
1166
7
5
1
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
82
81
69
52
45
38
37
32
23
23
23
20
20
20
20
20
19
19
18
18
17
16
15
14
14
14
14
14
12
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
5
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
2088
962
864
783
682
662
421
316
266
179
110
90
75
41
32
17
17
15
13
10
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN3043664
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN152249 CyTOF ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240 20
1 ABIN152309 ChIP FACS ICC IF IHC IHC (p) IP PLA WB Rabbit IgG AA 50-100 Log in to see Polyclonal 7
1 ABIN4342826 ICC IF IHC IHC (p) SimWes WB Mouse IgG1 Log in to see CL2199 0
1 ABIN6254862 ELISA ICC IF IHC WB Rabbit IgG acLys317 Log in to see Polyclonal 0
1 ABIN6256151 ELISA ICC IF IHC IP WB Rabbit IgG pSer15 Log in to see Polyclonal 0
1 ABIN6256312 ELISA ICC IF IHC WB Rabbit IgG pSer392 Log in to see Polyclonal 0
1 ABIN222890 ICC IP IHC WB Mouse IgG1 Ser20 Log in to see C6A5 2
1 ABIN2854816 ICC IF IP IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN4342783 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12 1
1 ABIN6255556 ELISA ICC IF IHC WB Rabbit IgG pSer9 Log in to see Polyclonal 0
1 ABIN6255078 ELISA ICC IF IHC WB Rabbit IgG pSer20 Log in to see Polyclonal 0
1 ABIN6255262 ELISA ICC IF IHC WB Rabbit IgG pSer366 Log in to see Polyclonal 0
1 ABIN6256267 ELISA ICC IF IHC IP WB Rabbit IgG pSer315 Log in to see Polyclonal 0
1 ABIN6263972 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 4
1 ABIN6263975 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2855525 ChIP ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
1 ABIN94449 ELISA ICC IHC (p) IP WB FITC Mouse IgG2a AA 16-25 Log in to see BP53-12 7
1 ABIN94448 ICC IHC (p) IP ELISA WB Mouse IgG2a AA 16-25 Log in to see BP53-12 7
1 ABIN6263970 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN1078633 ICC IHC WB Rabbit IgG AA 108-370 Log in to see Polyclonal 0

General

Antigen Tumor Protein P53 (TP53) Antibodies
Reactivity Human
(2533), (568), (476), (308), (162), (104), (103), (67), (56), (26), (23), (23), (18), (17), (17), (16), (7), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1)
Host Mouse
(1483), (1166), (7), (5), (1), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(82), (81), (69), (52), (45), (38), (37), (32), (23), (23), (23), (20), (20), (20), (20), (20), (19), (19), (18), (18), (17), (16), (15), (14), (14), (14), (14), (14), (12), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(2088), (962), (864), (783), (682), (662), (421), (316), (266), (179), (110), (90), (75), (41), (32), (17), (17), (15), (13), (10), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 28 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 antibody (ABIN3043664) Images
Purpose Mouse IgG monoclonal antibody for p53, tumor protein p53 (TP53) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Mouse IgG monoclonal antibody for p53, tumor protein p53 (TP53) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.
Gene Name: tumor protein p53
Protein Name: Cellular tumor antigen p53
Purification Ascites
Immunogen Recombinant human wild-type p53 protein.
Clone BP53-12
Isotype IgG2a
Plasmids, Primers & others

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 antibody (ABIN3043664) Images back to top
Antigen
Alternative Name TP53 (TP53 Antibody Abstract)
Background The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Synonyms: LFS1|TRP53|Li-Fraumeni syndrome|TRANSFORMATION-RELATED PROTEIN 53|Antigen NY-CO-13|Phosphoprotein p53|Tumor suppressor p53
Gene ID 7157, 22059, 24842
UniProt P04637, P02340, P10361
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 antibody (ABIN3043664) Images back to top
Application Notes WB: Concentration: 0.5-1 μg/mL, Species: Human
IHC-P: Concentration: 0.5-2 μg/mL, Species: Human
IHC-F: Concentration: 0.5-2 μg/mL, Species: Human
ICC: Concentration: 1 μg/mL, Species: Human
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921123 in WB, supported by ABIN921230 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 antibody (ABIN3043664) Images back to top
Format Lyophilized
Reconstitution Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.
Concentration 100 μg/mL
Buffer Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-p53 antibody (ABIN3043664)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Xiao, Zhou, Yi, Luo, Jiang, Tian, Li, Xue: "Fra-1 is downregulated in cervical cancer tissues and promotes cervical cancer cell apoptosis by p53 signaling pathway in vitro." in: International journal of oncology, Vol. 46, Issue 4, pp. 1677-84, 2015

Liang, Peng, Li, Yang, Xie, Li, Du, Zhang: "Silencing of CXCR4 sensitizes triple-negative breast cancer cells to cisplatin." in: Oncotarget, Vol. 6, Issue 2, pp. 1020-30, 2015

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, 2014

Dong, Wang: "Antitumor effects of artesunate on human breast carcinoma MCF-7 cells and IGF-IR expression in nude mice xenografts." in: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, Vol. 26, Issue 2, pp. 200-7, 2014

Wang, Xu, Zhou, Zhong, Wen, Yu, Chen, Shen, Chen, She, Jiang, Miao, Wei: "The viral oncoprotein HBx of Hepatitis B virus promotes the growth of hepatocellular carcinoma through cooperating with the cellular oncoprotein RMP." in: International journal of biological sciences, Vol. 10, Issue 10, pp. 1181-92, 2014

Zhang, Fu, Song, Wang, Lin, Liu: "Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application." in: Neural regeneration research, Vol. 7, Issue 30, pp. 2347-53, 2014

Liao, Xiao, Zhu, Zheng, He, Pei, Li, Zhou: "CD38 is highly expressed and affects the PI3K/Akt signaling pathway in cervical cancer." in: Oncology reports, Vol. 32, Issue 6, pp. 2703-9, 2014

Li, Wu, Wei, Wang, Sun, Li, Zhang, Zhang, Xin: "Anti-tumor effect of cactus polysaccharides on lung squamous carcinoma cells (SK-MES-1)." in: African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines, Vol. 11, Issue 5, pp. 99-104, 2014

Li, Wang, Li, Wang, Wang, Li, Han, Wang, Ma, Wang: "Proteomics analysis of normal and senescent NG108-15 cells: GRP78 plays a negative role in cisplatin-induced senescence in the NG108-15 cell line." in: PLoS ONE, Vol. 9, Issue 3, pp. e90114, 2014

Li, Wang, Dong, Li, Li, Han, Han, Wang, Ma, Wang: "Cisplatin-induced senescence in ovarian cancer cells is mediated by GRP78." in: Oncology reports, Vol. 31, Issue 6, pp. 2525-34, 2014

Zhang, Chen, Shi, Xie, Liu, Zhang, Lai, Zuo, Chen, Liu, Wang: "Establishment of a new representative model of human ovarian cancer in mice." in: Journal of ovarian research, Vol. 6, Issue 1, pp. 9, 2013

Yang, Wang, Guo, Chen, Zhang, Shen, Yu, Miao, Wang, Wei: "RMP plays distinct roles in the proliferation of hepatocellular carcinoma cells and normal hepatic cells." in: International journal of biological sciences, Vol. 9, Issue 6, pp. 637-48, 2013

Zhang, Kong, Zhang, Lei, Zhu, Zhang, Chen, Lu, Zheng: "Tetramethylpyrazine induces G0/G1 cell cycle arrest and stimulates mitochondrial-mediated and caspase-dependent apoptosis through modulating ERK/p53 signaling in hepatic stellate cells in vitro." in: Apoptosis : an international journal on programmed cell death, Vol. 18, Issue 2, pp. 135-49, 2013

Hao, Huang, Liao, Zhu, Liu, Hao, Liu, Zhang, Feng, Ning, Li, Li: "The inhibition of resveratrol to human skin squamous cell carcinoma A431 xenografts in nude mice." in: Fitoterapia, Vol. 86, pp. 84-91, 2013

Zhou, Yang, Tian, Wang: "Oxidative stress-induced 1, N6-ethenodeoxyadenosine adduct formation contributes to hepatocarcinogenesis." in: Oncology reports, Vol. 29, Issue 3, pp. 875-84, 2013

Liu, Yang, Yuan, Zhai, Huo: "SUMOylation of mouse p53b by SUMO-1 promotes its pro-apoptotic function in ovarian granulosa cells." in: PLoS ONE, Vol. 8, Issue 5, pp. e63680, 2013

Bai, Lei, Zhang, Zhao, Liang: "Sulfite exposure-induced hepatocyte death is not associated with alterations in p53 protein expression." in: Toxicology, Vol. 312, pp. 142-8, 2013

Li, Xu, Li, Zou, Saimaiti, Simayi, Wang, Zhang, Yuan, Zhang: "Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo." in: Cellular signalling, Vol. 24, Issue 3, pp. 718-25, 2012

Guan, Li, Yang, Huang, Zheng, Zhang, Li, Liu: "Cell cycle arrest and apoptosis of OVCAR-3 and MCF-7 cells induced by co-immobilized TNF-? plus IFN-? on polystyrene and the role of p53 activation." in: Biomaterials, Vol. 33, Issue 26, pp. 6162-71, 2012

Zhang, Zhang, Li, Wang, Yue, Xie: "miR-98 regulates cisplatin-induced A549 cell death by inhibiting TP53 pathway." in: Biomedicine & pharmacotherapy = Biome?decine & pharmacothe?rapie, Vol. 65, Issue 6, pp. 436-42, 2011

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 antibody (ABIN3043664) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Protein P53 (TP53) antibody (ABIN3043664) Anti-p53 antibody (monoclonal), IHC(P) IHC(P): Human Mammary Cancer Tissue
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) antibody (ABIN3043664) Anti-p53 antibody (monoclonal), Western blotting WB: HL-60 Cell Lysate