anti-Human p53 antibody for Immunofluorescence

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
Epitope
Center
169
125
78
74
72
71
70
59
58
54
46
45
43
36
36
34
33
25
24
24
22
22
21
21
17
15
15
15
15
15
15
15
13
13
13
12
11
10
9
9
8
8
8
8
7
7
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2506
567
465
308
162
104
103
67
56
26
23
23
18
17
17
16
7
6
5
4
4
4
4
3
3
2
2
1
1
Host
Rabbit
1466
1156
7
5
1
1
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
81
79
68
52
45
36
35
32
23
23
23
20
20
20
20
20
19
19
18
18
15
14
14
12
12
12
12
12
11
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
5
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
2083
961
864
782
682
658
419
315
267
155
110
90
75
41
32
17
16
15
13
10
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN2855525
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
29.063921 ABIN2854816 ICC IF IP IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
29.063921 ABIN3181122 IF IHC WB Mouse IgG1 Log in to see 6C4 0
26.063921 ABIN3020520 IF IHC WB Rabbit pSer315 Log in to see Polyclonal 0
26.063921 ABIN3019668 IF IHC WB Rabbit pSer15 Log in to see Polyclonal 0
23.308239 ABIN394129 IF IHC IP WB Mouse IgG1 kappa AA 94-202 Log in to see 2C3 0
23.308239 ABIN1875167 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
23.308239 ABIN1875168 IF WB Rabbit IgG Log in to see Polyclonal 0
23.308239 ABIN2564348 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
23.308239 ABIN563227 IF IHC (p) IP ELISA WB Mouse IgG1 kappa AA 94-201, partial Log in to see 2C3 0
23.063921 ABIN6254176 IF WB Goat IgG C-Term Log in to see Polyclonal 0
23.063921 ABIN6254175 IF WB Goat IgG C-Term Log in to see Polyclonal 0
23.063921 ABIN3020523 IF WB Rabbit pSer37 Log in to see Polyclonal 0
23.063921 ABIN3019677 IF WB Rabbit pSer46 Log in to see Polyclonal 0
19 ABIN6254862 ELISA ICC IF IHC WB Rabbit IgG acLys317 Log in to see Polyclonal 0
19 ABIN6256151 ELISA ICC IF IHC IP WB Rabbit IgG pSer15 Log in to see Polyclonal 0
19 ABIN6256312 ELISA ICC IF IHC WB Rabbit IgG pSer392 Log in to see Polyclonal 0
19 ABIN1499967 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 3A6 0
19 ABIN6255078 ELISA ICC IF IHC WB Rabbit IgG pSer20 Log in to see Polyclonal 0
19 ABIN1499971 FACS IF IHC IHC (p) WB Mouse IgG2b Log in to see 3F5 0
19 ABIN1499969 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 1C6 0

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope Center
(169), (125), (78), (74), (72), (71), (70), (59), (58), (54), (46), (45), (43), (36), (36), (34), (33), (25), (24), (24), (22), (22), (21), (21), (17), (15), (15), (15), (15), (15), (15), (15), (13), (13), (13), (12), (11), (10), (9), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2506), (567), (465), (308), (162), (104), (103), (67), (56), (26), (23), (23), (18), (17), (17), (16), (7), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1)
Host Rabbit
(1466), (1156), (7), (5), (1), (1), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(81), (79), (68), (52), (45), (36), (35), (32), (23), (23), (23), (20), (20), (20), (20), (20), (19), (19), (18), (18), (15), (14), (14), (12), (12), (12), (12), (12), (11), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (2), (2), (2)
Application Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(2083), (961), (864), (782), (682), (658), (419), (315), (267), (155), (110), (90), (75), (41), (32), (17), (16), (15), (13), (10), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to p53 (tumor protein p53)
p53 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human p53. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details p53

Product Details anti-p53 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.

Cellular Localization: Cytoplasm , Nucleus , Endoplasmic reticulum
Molecular Weight 44 kDa
Gene ID 7157
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling Images back to top
Application Notes Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HCT116 24hr cisplatin 30uM , *HUVEC

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Protein P53 (TP53) (Center) antibody (ABIN2855525) IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ...
Immunofluorescence (IF) image for anti-Tumor Protein P53 (TP53) (Center) antibody (ABIN2855525) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde...
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (Center) antibody (ABIN2855525) WB Image Sample (30 ug of whole cell lysate) A: A431 , 10% SDS PAGE antibody diluted ...
Immunoprecipitation (IP) image for anti-Tumor Protein P53 (TP53) (Center) antibody (ABIN2855525) IP Image Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treat...
Immunofluorescence (IF) image for anti-Tumor Protein P53 (TP53) (Center) antibody (ABIN2855525) ICC/IF Image p53 antibody detects p53 protein at nucleus by immunofluorescent analysi...
Chromatin Immunoprecipitation (ChIP) image for anti-Tumor Protein P53 (TP53) (Center) antibody (ABIN2855525) ChIP Image p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiment...