|Antigen||Tumor Protein P53 (TP53) Antibodies|
|Epitope||AA 371-380 Alternatives|
|Reactivity||Human, Monkey, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This p53 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-p53 AntibodyTarget Details p53 Application Details Handling Images
|Specificity||This antibody reacts to p53.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Monkey, Mouse and Rat.|
|Characteristics||Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13|
|Purification||Affinity chromatography on Protein A/G|
|Immunogen||Hybridoma produced by the fusion of splenocytes from immunized with full length humanp53 protein and mouse myeloma cells.|
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|Alternative Name||p53 (TP53) (TP53 Antibody Abstract)|
|Background||P53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53|
|Pathways||p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy|
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Western Blot (1-5 μg/mL). ELISA. Immunoprecipitation. Immunohistochemistry. Positive Control: Breast or colon carcinoma cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||PBS, 0.08 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|
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