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|Antigen||Tumor Protein P53 (TP53) Antibodies|
|Epitope||AA 16-25 Alternatives|
|Reactivity||Human, Mouse (Murine), Xenopus laevis Alternatives|
|Conjugate||This p53 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-p53 AntibodyTarget Details p53 Application Details Handling References for anti-p53 antibody (ABIN264401) Images
|Specificity||This antibody detects p53 protein.|
|Characteristics||Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13|
|Immunogen||Hybridoma produced by the fusion of splenocytes from immunized with full lengthXenopus p53 protein and mouse myeloma cells.|
Target Details p53Product Details anti-p53 Antibody Application Details Handling References for anti-p53 antibody (ABIN264401) Images back to top
|Alternative Name||p53 (TP53) (TP53 Antibody Abstract)|
|Background||P53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53|
|Pathways||p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy|
Application DetailsProduct Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 antibody (ABIN264401) Images back to top
Western Blot: 1-10 μg/mL, detects a band a approximately 53 kDa in HCT116 cell lysate. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 antibody (ABIN264401) Images back to top
|Buffer||PBS containing 0.08 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|
References for anti-p53 antibody (ABIN264401)Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Hussain, Hollstein, Harris: "p53 tumor suppressor gene: at the crossroads of molecular carcinogenesis, molecular epidemiology, and human risk assessment." in: Annals of the New York Academy of Sciences, Vol. 919, pp. 79-85, 2000
Hellin, Bentires-Alj, Verlaet, Benoit, Gielen, Bours, Merville: "Roles of nuclear factor-kappaB, p53, and p21/WAF1 in daunomycin-induced cell cycle arrest and apoptosis." in: The Journal of pharmacology and experimental therapeutics, Vol. 295, Issue 3, pp. 870-8, 2000
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