anti-Primate p53 antibody for Flow Cytometry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
Epitope
AA 37-45
175
122
89
81
71
71
70
58
57
52
49
47
46
45
41
37
35
31
25
22
22
20
17
16
15
15
15
15
15
15
14
12
11
11
10
10
10
9
8
7
7
7
7
7
6
6
6
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Primate
2331
506
428
182
118
92
84
83
33
23
20
20
20
16
15
14
6
4
4
4
3
3
3
2
1
1
Host
Mouse
1333
1108
7
1
1
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
82
77
64
52
46
45
45
31
31
26
25
25
25
25
24
24
24
23
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1877
762
739
709
623
604
411
341
308
179
72
41
32
30
21
17
17
15
13
9
9
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4342822
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4342820 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342819 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7 0
1 ABIN4342821 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342817 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7 0
1 ABIN4342818 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7 0
1 ABIN4342792 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122 0
1 ABIN4342793 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122 0
1 ABIN4342794 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122 0
1 ABIN4342816 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2b kappa AA 37-45 Log in to see DO-7 0
1 ABIN4342836 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342837 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342839 FACS APC Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342841 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342842 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342845 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342840 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342846 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342847 FACS PE Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342848 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgG2b kappa AA 37-45 Log in to see SPM590 0
1 ABIN4342814 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2b kappa AA 37-45 Log in to see DO-7 0

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 37-45
(175), (122), (89), (81), (71), (71), (70), (58), (57), (52), (49), (47), (46), (45), (41), (37), (35), (31), (25), (22), (22), (20), (17), (16), (15), (15), (15), (15), (15), (15), (14), (12), (11), (11), (10), (10), (10), (9), (8), (7), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Primate
(2331), (506), (428), (182), (118), (92), (84), (83), (33), (23), (20), (20), (20), (16), (15), (14), (6), (4), (4), (4), (3), (3), (3), (2), (1), (1)
Host Mouse
(1333), (1108), (7), (1), (1), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(82), (77), (64), (52), (46), (45), (45), (31), (31), (26), (25), (25), (25), (25), (24), (24), (24), (23), (20), (19), (18), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1877), (762), (739), (709), (623), (604), (411), (341), (308), (179), (72), (41), (32), (30), (21), (17), (17), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling Images
Specificity Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 37-45) of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Purification Protein A purified
Immunogen Recombinant human wild type p53 protein expressed in E. coli.
Clone SPM590
Isotype IgG2b kappa

Target Details p53

Product Details anti-p53 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background Gene Symbol: TP53
Molecular Weight Theoretical MW: 53 kDa
Gene ID 7157
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling Images back to top
Application Notes Western Blot 0.5-1.0 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunocytochemistry/Immunofluorescence 0.5-1 μg/mL, Immunoprecipitation 0.5-1 μg/500 μg, Immunohistochemistry-Paraffin 0.5-1.0 μg/mL, Immunohistochemistry-Frozen 0.5-1.0 μg/mLHuman Chromosome Location: 17p13.1 Immunohistochemistry (Staining of paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.