anti-Mouse (Murine) PAG1 antibody for Immunoprecipitation

Recommended PAG1 Antibody (supplied by: Log in to see )

Antigen
phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
  • Cbp
  • F730007C19Rik
  • Pag
  • CBP
  • PAG
  • MGC80192
  • phosphoprotein associated with glycosphingolipid microdomains 1
  • phosphoprotein membrane anchor with glycosphingolipid microdomains 1
  • phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog
  • Pag1
  • PAG1
  • pag1.L
  • pag1
Epitope
C-Term
32
25
16
4
3
2
2
2
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
100
62
46
28
2
2
1
1
1
Host
Mouse
54
54
Clonality
Monoclonal
Conjugate
This PAG1 antibody is un-conjugated
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
69
55
47
31
23
13
11
6
3
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN94270
$ 316.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.614769 ABIN269119 IHC (p) IP IHC WB Mouse IgG2b C-Term Log in to see PAG-C1 1
1 ABIN351516 IHC IHC (p) IP WB Mouse IgG2b C-Term Log in to see PAG-C1 0
1 ABIN351518 IHC IHC (p) IP WB Rabbit AA 97-432 Log in to see Polyclonal 0
1 ABIN372975 IHC (p) IP WB Rabbit AA 97-432 Log in to see Polyclonal 0
1 ABIN1723396 IHC (p) IP Mouse IgG2b C-Term Log in to see PAG-C1 0
1 ABIN1737819 IHC (p) IP WB Rabbit IgG AA 97-432 Log in to see Polyclonal 0
1 ABIN2470738 IP IHC Mouse IgG2b C-Term Log in to see PAG-C1 0
1 ABIN94497 IHC (p) IP WB Rabbit IgG AA 97-432 Log in to see Polyclonal 0
1 ABIN596909 IP Mouse IgG2b C-Term Log in to see PAG-C1 0
1 ABIN2470740 IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN199042 IP WB Rabbit AA 97-432 Log in to see Polyclonal 0
1 ABIN120704 IHC (p) IP Mouse IgG2b C-Term Log in to see PAG-C1 0
1 ABIN269172 IHC (p) IP IHC WB Rabbit AA 97-432 Log in to see Polyclonal 0
1 ABIN2743437 IHC IHC (p) IP WB Rabbit AA 97-432 Log in to see Polyclonal 0
1 ABIN2582424 ICC IP WB Rabbit AA 412-424 Log in to see Polyclonal 0
1 ABIN449149 IP Mouse IgG2b C-Term Log in to see PAG-C1 0
1 ABIN5689872 ICC IP WB Rabbit AA 412-424 Log in to see Polyclonal 0
1 ABIN3200740 IHC (p) IP WB Mouse IgG2b C-Term Log in to see PAG-C1 0
1 ABIN2745550 ICC IP WB Rabbit AA 412-424, N-Term Log in to see Polyclonal 0
1 ABIN121943 IHC (p) IP WB Rabbit AA 97-432 Log in to see Polyclonal 0

General

Antigen phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
Epitope C-Term
(32), (25), (16), (4), (3), (2), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
(100), (62), (46), (28), (2), (2), (1), (1), (1)
Host Mouse
(54), (54)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PAG1 antibody is un-conjugated
(4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(69), (55), (47), (31), (23), (13), (11), (6), (3), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-PAG1 Antibody

Target Details PAG1 Application Details Handling References for anti-PAG1 antibody (ABIN94270) Images
Specificity The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Clone PAG-C1
Isotype IgG2b
Plasmids, Primers & others

Target Details PAG1

Product Details anti-PAG1 Antibody Application Details Handling References for anti-PAG1 antibody (ABIN94270) Images back to top
Antigen
Alternative Name PAG1 (PAG1 Antibody Abstract)
Background PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Pathways p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events

Application Details

Product Details anti-PAG1 Antibody Target Details PAG1 Handling References for anti-PAG1 antibody (ABIN94270) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details References for anti-PAG1 antibody (ABIN94270) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-PAG1 antibody (ABIN94270)

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling Images back to top
Background publications

Simeoni, Smida, Posevitz, Schraven, Lindquist: "Right time, right place: the organization of membrane proximal signaling." in: Seminars in immunology, Vol. 17, Issue 1, pp. 35-49, 2004

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004

Baumgartner, Angelisová, Setterblad, Mooney, Werling, Horejsí, Langsley: "Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes." in: Blood, Vol. 101, Issue 5, pp. 1874-81, 2003

Product cited in:

Davidson, Bakinowski, Thomas, Horejsi, Veillette: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, 2003

Vang, Abrahamsen, Myklebust, Horejsí, Taskén: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, 2003

Images

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling References for anti-PAG1 antibody (ABIN94270) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibody (ABIN94270) Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma c...
Immunohistochemistry (IHC) image for anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibody (ABIN94270) Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of ly...