anti-Mouse (Murine) PAG1 antibody for Western Blotting
Recommended PAG1 Antibody (supplied by: Log in to see )
- Antigen
-
phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
show synonyms for this antigen- Cbp
- F730007C19Rik
- Pag
- CBP
- PAG
- MGC80192
- phosphoprotein associated with glycosphingolipid microdomains 1
- phosphoprotein membrane anchor with glycosphingolipid microdomains 1
- phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog
- Pag1
- PAG1
- pag1.L
- pag1
- Epitope
-
C-Term
Alternatives - Reactivity
-
Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
Alternatives - Host
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Mouse
Alternatives - Clonality
- Conjugate
-
This PAG1 antibody is un-conjugated
Alternatives - Application
-
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives - Options
- Supplier
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- Supplier Product No.
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 15.767978 | ABIN94497 | IHC (p) IP WB | Rabbit | IgG | AA 97-432 | Log in to see | Polyclonal | 0 | ||
| 12.767978 | ABIN2745550 | ICC IP WB | Rabbit | AA 412-424, N-Term | Log in to see | Polyclonal | 0 | |||
| 10.800212 | ABIN5014125 | ICC IHC WB | Rabbit | IgG | AA 160-390 | Log in to see | Polyclonal | 0 | ||
| 10.800212 | ABIN2920069 | IF/ICC IHC IP WB | Rabbit | AA 160-390 | Log in to see | Polyclonal | 0 | |||
| 7 | ABIN351516 | IHC IHC (p) IP WB | Mouse | IgG2b | C-Term | Log in to see | PAG-C1 | 0 | ||
| 7 | ABIN351518 | IHC IHC (p) IP WB | Rabbit | AA 97-432 | Log in to see | Polyclonal | 0 | |||
| 4.8002124 | ABIN2789626 | WB | Rabbit | C-Term | Log in to see | Polyclonal | 0 | |||
| 4 | ABIN799584 | WB | Rabbit | C-Term | Log in to see | Polyclonal | 0 | |||
| 4 | ABIN372975 | IHC (p) IP WB | Rabbit | AA 97-432 | Log in to see | Polyclonal | 0 | |||
| 4 | ABIN1737819 | IHC (p) IP WB | Rabbit | IgG | AA 97-432 | Log in to see | Polyclonal | 0 | ||
| 4 | ABIN2470740 | IP IHC WB | Rabbit | C-Term | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN199042 | IP WB | Rabbit | AA 97-432 | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN2743437 | IHC IHC (p) IP WB | Rabbit | AA 97-432 | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN2582424 | ICC IP WB | Rabbit | AA 412-424 | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN3200740 | IHC (p) IP WB | Mouse | IgG2b | C-Term | Log in to see | PAG-C1 | 0 | ||
| 1 | ABIN5689872 | ICC IP WB | Rabbit | AA 412-424 | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN121943 | IHC (p) IP WB | Rabbit | AA 97-432 | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN6090011 | ELISA IF/ICC IHC WB | Rabbit | IgG | AA 97-432 | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN5897815 | ELISA WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN5700563 | ELISA WB | Rabbit | IgG | Log in to see | Polyclonal | 0 |
General |
|
|---|---|
| Antigen | phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies |
| Epitope | C-Term Alternatives |
| Reactivity | Human, Mouse (Murine), Rat (Rattus), Cow (Bovine) Alternatives |
| Host | Mouse Alternatives |
| Clonality (Clone) | |
| Conjugate | This PAG1 antibody is un-conjugated Alternatives |
| Application |
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
|
|
5 references available |
| Supplier | Log in to see |
Product Details anti-PAG1 AntibodyTarget Details PAG1 Application Details Handling References for anti-PAG1 antibody (ABIN94270) Images |
|
| Specificity | The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. |
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95 % (by SDS-PAGE) |
| Immunogen | C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH. |
| Clone | PAG-C1 |
| Isotype | IgG2b |
| Plasmids, Primers & others | |
Target Details PAG1Product Details anti-PAG1 Antibody Application Details Handling References for anti-PAG1 antibody (ABIN94270) Images back to top |
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| Antigen | |
| Alternative Name | PAG1 (PAG1 Antibody Abstract) |
| Background | PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck. |
| Pathways | p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events |
Application DetailsProduct Details anti-PAG1 Antibody Target Details PAG1 Handling References for anti-PAG1 antibody (ABIN94270) Images back to top |
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| Application Notes | Optimal working dilution should be determined by the investigator. |
| Restrictions | For Research Use only |
HandlingProduct Details anti-PAG1 Antibody Target Details PAG1 Application Details References for anti-PAG1 antibody (ABIN94270) Images back to top |
|
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Handling Advice | Do not freeze. |
| Storage | 4 °C |
| Storage Comment | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
References for anti-PAG1 antibody (ABIN94270)Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling Images back to top |
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| Background publications |
Simeoni, Smida, Posevitz, Schraven, Lindquist: "Right time, right place: the organization of membrane proximal signaling." in: Seminars in immunology, Vol. 17, Issue 1, pp. 35-49, 2004 Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004 Baumgartner, Angelisová, Setterblad, Mooney, Werling, Horejsí, Langsley: "Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes." in: Blood, Vol. 101, Issue 5, pp. 1874-81, 2003 |
| Product cited in: |
Davidson, Bakinowski, Thomas, Horejsi, Veillette: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, 2003 Vang, Abrahamsen, Myklebust, Horejsí, Taskén: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, 2003 |
ImagesProduct Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling References for anti-PAG1 antibody (ABIN94270) back to top |
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| Supplier Images |
Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma c...
Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of ly...
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