anti-Human SERPINE1 antibody for Enzyme Immunoassay

Recommended SERPINE1 Antibody

Antigen
serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies
  • PAI
  • PAI-1
  • PAI1
  • PLANH1
  • SERPINE1
  • si:ch211-138a11.1
  • Planh1
  • PAI1A
  • Pai1
  • Pai1aa
  • Planh
  • RATPAI1A
  • serpin family E member 1
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • serine (or cysteine) peptidase inhibitor, clade E, member 1
  • SERPINE1
  • serpine1.L
  • serpine1
  • Serpine1
Epitope
Internal Region
19
15
13
11
8
8
8
7
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
356
127
118
42
17
11
7
6
5
4
3
2
2
1
1
Host
Goat
267
129
51
28
2
1
1
Clonality
Polyclonal
Conjugate
This SERPINE1 antibody is un-conjugated
48
31
16
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
Application
ELISA, Enzyme Immunoassay (EIA), Immunohistochemistry (IHC), Western Blotting (WB)
333
217
141
55
43
30
26
25
23
18
17
9
9
6
6
6
4
4
4
4
3
3
1
1
1
Options

Available images

Catalog No. ABIN185384
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
13.501226 ABIN5539802 ELISA EIA IHC WB Biotin Goat Internal Region Polyclonal 0
13 ABIN2564364 EIA IHC ELISA WB Goat IgG Internal Region Polyclonal 0
10 ABIN615419 EIA IHC (p) WB Goat Internal Region Polyclonal 0
7 ABIN4952038 ELISA EIA IHC WB Goat Ig Fraction Polyclonal 0
5.5 ABIN5553071 EIA IHC (p) WB Goat Internal Region Polyclonal 1
4 ABIN5609774 ELISA EIA IHC WB Biotin Goat Internal Region Polyclonal 0
4 ABIN4952039 ELISA EIA IHC WB Biotin Goat Ig Fraction Polyclonal 0
1 ABIN5556327 EIA WB Rabbit Ig Fraction AA 187-216, Middle Region Polyclonal 0
1 ABIN1108976 Func EIA WB Mouse IgG1 MA-33H1F7 5
1 ABIN264029 EIA Mouse IgG 1-79769313486232E-308 0
1 ABIN1108977 Func EIA IHC (fro) WB Mouse IgG1 MA-33H1F7 3
1 ABIN191654 EIA IHC (fro) IHC (p) RIA Goat Polyclonal 0
1 ABIN1108975 Func EIA Mouse IgG1 MA-55F4C12 3
1 ABIN5541683 EIA FACS IHC (p) WB Mouse IgG1 1D5 0
1 ABIN191652 EIA IHC (fro) IHC (p) RIA Goat Polyclonal 0
1 ABIN264710 EIA IHC (fro) WB Rabbit Polyclonal 0
1 ABIN613279 EIA RIA Biotin Rabbit IgG Polyclonal 0
1 ABIN613753 EIA RIA Rabbit IgG Polyclonal 0

General

Antigen serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies
Epitope Internal Region
(19), (15), (13), (11), (8), (8), (8), (7), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(356), (127), (118), (42), (17), (11), (7), (6), (5), (4), (3), (2), (2), (1), (1)
Host Goat
(267), (129), (51), (28), (2), (1), (1)
Clonality
Polyclonal
Conjugate This SERPINE1 antibody is un-conjugated
(48), (31), (16), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1)
Application ELISA, Enzyme Immunoassay (EIA), Immunohistochemistry (IHC), Western Blotting (WB)
(333), (217), (141), (55), (43), (30), (26), (25), (23), (18), (17), (9), (9), (6), (6), (6), (4), (4), (4), (4), (3), (3), (1), (1), (1)
Pubmed 2 references available
Supplier

Product Details anti-SERPINE1 Antibody

Target Details SERPINE1 Application Details Handling References for anti-SERPINE1 antibody (ABIN185384) Images
Purpose PAI1 / SERPINE1
Blocking Peptide Blocking peptide for this product available: ABIN369114
Sequence GFKIDDKGMA PALRH
Predicted Reactivity Human, Dog
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-GFKIDDKGMAPALRH, from the internal region of the protein sequence according to NP_000593.1, NP_001158885.1.
Plasmids, Primers & others

Target Details SERPINE1

Product Details anti-SERPINE1 Antibody Application Details Handling References for anti-SERPINE1 antibody (ABIN185384) Images back to top
Antigen
Alternative Name SERPINE1 (SERPINE1 Antibody Abstract)
Background SERPINE1, PAI1, PAI, PAI-1, PLANH1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor, type I, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor-1
Molecular Weight 45.1kDa
Gene ID 5054
NCBI Accession NP_000593
Pathways p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration

Application Details

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Handling References for anti-SERPINE1 antibody (ABIN185384) Images back to top
Application Notes

DS WB Results: Approx 48 kDa band observed in Human Liver and HeLa lysates, while approx. 45 kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1 kDa according to NP_000593.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 μg/mL.

DS IHC Results: In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration: 4-6 μg/mL. A customer successfully used this product on Human Breast cancer.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:64000.

Comment

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAI1 captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml

Restrictions For Research Use only

Handling

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details References for anti-SERPINE1 antibody (ABIN185384) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-SERPINE1 antibody (ABIN185384)

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling Images back to top
Product cited in:

Kozaczuk, Selmi, Bednarek: "Bacterial expression, purification and angiogenesis-promoting activity of human thymosin ?4." in: Protein expression and purification, Vol. 90, Issue 2, pp. 142-52, 2013

Background publications

Baumruker, Csonga, Pursch, Pfeffer, Urtz, Sutton, Bofill-Cardona, Cooke, Prieschl: "Activation of mast cells by incorporation of cholesterol into rafts." in: International immunology, Vol. 15, Issue 10, pp. 1207-18, 2003

Images

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling References for anti-SERPINE1 antibody (ABIN185384) back to top
Supplier Images
 image for anti-serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) (Internal Region) antibody (ABIN185384) ABIN185384 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). P...